Merck
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35845

Sigma-Aldrich

2′,7′-二氯荧光素二乙酸酯

BioReagent, suitable for fluorescence, ≥95% (HPLC)

Synonym(s):
Dcfh-DA, DCFHDA, Diacetyldichlorofluorescein
Empirical Formula (Hill Notation):
C24H14Cl2O7
CAS Number:
Molecular Weight:
485.27
Beilstein:
68488
MDL编号:
PubChem化学物质编号:
NACRES:
NA.32

质量水平

100

产品线

BioReagent

测定

≥95% (HPLC)

mp

232-234 °C (lit.)

溶解性

DMSO: soluble

荧光

λex 504 nm; λem 524 nm in 0.1 M Tris pH 8.0 (esterase)

适用性

suitable for fluorescence

储存温度

2-8°C

SMILES string

CC(=O)Oc1cc2Oc3cc(OC(C)=O)c(Cl)cc3C4(OC(=O)c5ccccc45)c2cc1Cl

InChI

1S/C24H14Cl2O7/c1-11(27)30-21-9-19-15(7-17(21)25)24(14-6-4-3-5-13(14)23(29)33-24)16-8-18(26)22(31-12(2)28)10-20(16)32-19/h3-10H,1-2H3

InChI key

VQVUBYASAICPFU-UHFFFAOYSA-N

一般描述

2′,7′-Dichlorofluorescein diacetate, also known as DCFHDA or Dcfh-DA, is a sensitive cell-permeable redox probe. The lipophilic non-fluorescent Dcfh-DA readily crosses the cell membrane through passive diffusion followed by deacetylation. The deacylated product is an oxidant sensitive 2′,7′-dichlorofluorescein (DCHF). DCHF is oxidized later to form highly fluorescent DCF, which is measured.

应用

2′,7′-二氯荧光素二乙酸酯可用作未衍生化荧光素的优良气相替代物,并可用作2′,7′-二氯荧光素二乙酸酯测定的组分/底物,以定量活性氧物质 (ROS)。

储存分类代码

13 - Non Combustible Solids

WGK

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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