71376

氯化钠

BioUltra, for molecular biology, ≥99.5% (AT)

• 别名: NaCl, 岩盐
• 线性分子式: NaCl
• CAS号: 7647-14-5
• 分子量: 58.44
• Beilstein: 3534976
• EC 号: 231-598-3
• MDL编号: MFCD00003477
• PubChem化学物质编号: 57652294
• NACRES: NA.25

属性

• 等级: for molecular biology
• 质量水平: 200
• 蒸汽压: 1.33 (1.00 mmHg) at 865 °C hPa
• 产品线: BioUltra
• 检测方案: ≥99.5% (AT)
• 形式: solid
• availability: not available in (Sales restrictions may apply)
• 杂质: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected; ≤0.001% total nitrogen (N)
• pH值（酸碱度）: 5.0-8.0 (25 °C, 1 M in H₂O)
• mp: 801 °C (lit.)
• 溶解性: H₂O: 1 M at 20 °C, clear, colorless
• 痕量阴离子: bromide (Br^-): ≤50 mg/kg; hexacyanoferrate(II) ([Fe(CN)₆]^4-): ≤1 mg/kg; iodide (I^-): ≤10 mg/kg; phosphate (PO₄^3-): ≤5 mg/kg; sulfate (SO₄^2-): ≤200 mg/kg
• 痕量阳离子: Al: ≤5 mg/kg; As: ≤0.1 mg/kg; Ba: ≤5 mg/kg; Bi: ≤5 mg/kg; Ca: ≤10 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤1 mg/kg; K: ≤50 mg/kg; Li: ≤5 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Mo: ≤5 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Sr: ≤5 mg/kg; Zn: ≤5 mg/kg
• SMILES string: [Na+].[Cl-]
• λ: 1 M in H₂O
• 紫外吸收: λ: 260 nm A_max: ≤0.01; λ: 280 nm A_max: ≤0.01
• 适用性: suitable for molecular biology
• InChI: 1S/ClH.Na/h1H;/q;+1/p-1
• InChI key: FAPWRFPIFSIZLT-UHFFFAOYSA-M

说明

一般描述

Sodium chloride, an essential nutrient, plays a critical role in biochemistry and molecular biology, making significant contributions to various biochemical applications. Within biological systems, it serves as a fundamental electrolyte, regulating osmotic pressure, pH balance, and electrical conductivity, all of which are essential for proper cellular function and vital physiological processes. Furthermore, sodium chloride plays a pivotal part in maintaining protein structure and promoting enzymatic activity, essential for stabilizing protein conformations and catalyzing biochemical reactions. Its role extends to the regulation of cellular homeostasis and the facilitation of ion and nutrient transport across cell membranes.It is an integral component of buffers like PBS and SSC, where it aids in various biochemical procedures, including the creation of density gradients and the fine-tuning of biological functions. Notably, sodium chloride is used to increase the ionic strength in buffers and culture media, impacting the ionic environment crucial for specific experiments and cell culture. Moreover, it is applied in DNA precipitation from samples containing SDS and the removal of small nucleic acid fragments during plasmid DNA preparations. Its utility extends to cell culture applications, where it plays a role in bacterial, yeast, and cell culture growth media.In molecular biology and cell culture, sodium chloride is an indispensable salt that finds its place in various aspects of research. It is a common constituent in cell culture media, lysis buffers, and protein storage solutions, contributing to the maintenance of optimal conditions for cell growth and experimentation. The sodium chloride used in these applications is of high purity, rigorously tested for impurities such as DNases, RNases, phosphatases, proteases, and trace metals, making it the ideal choice for molecular biology and biochemical research

应用

氯化钠已用于：

• 作为制备pH溶液的缓冲液组分，用于开路电位和循环伏安法
• 制备Bold基础培养基(BBM)
• 作为转录和退火缓冲液

生化/生理作用

氯化钠有助于激活辅助性T细胞17(TH17)细胞的稳定诱导。

特点和优势

• Suitable for molecular biology and biochecmial research
• Free from DNase, RNase, NICKase and protease
• Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications.
• Tested for the presence of Anionic and Cationic traces

其他说明

This product is intended for research purposes only, and it is not meant for human consumption.

可能会涉及到销售限制

大规模苯酚提取RNA的裂解缓冲液和提取缓冲液的组分；从人类有核细胞中提取DNA的简单盐析程序

安全信息

• 储存分类代码: 13 - Non Combustible Solids
• WGK: WGK 1
• 闪点(F): Not applicable
• 闪点(C): Not applicable
• 个人防护装备: Eyeshields, Gloves, type N95 (US)