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Merck

71376

氯化钠

BioUltra, Molecular Biology, ≥99.5% (AT)

别名:

盐, NaCl, 岩盐

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$283.00

5 KG

$745.00

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关于此项目

线性分子式:
NaCl
化学文摘社编号:
分子量:
58.44
UNSPSC Code:
12352302
NACRES:
NA.25
PubChem Substance ID:
EC Number:
231-598-3
Beilstein/REAXYS Number:
3534976
MDL number:
Assay:
≥99.5% (AT)
Grade:
Molecular Biology
Form:
solid
Solubility:
H2O: 1 M at 20 °C, clear, colorless

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InChI key

FAPWRFPIFSIZLT-UHFFFAOYSA-M

InChI

1S/ClH.Na/h1H;/q;+1/p-1

SMILES string

[Na+].[Cl-]

grade

Molecular Biology

vapor pressure

1.33 (1.00 mmHg) at 865 °C hPa

product line

BioUltra

assay

≥99.5% (AT)

form

solid

availability

not available in (Sales restrictions may apply)

technique(s)

mass spectrometry (MS): suitable, western blot: suitable

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected, ≤0.001% total nitrogen (N)

pH

5.0-8.0 (25 °C, 1 M in H2O)

mp

801 °C (lit.)

solubility

H2O: 1 M at 20 °C, clear, colorless

density

2.16 g/cm3 at 25 °C (77 °F)

anion traces

bromide (Br-): ≤50 mg/kg, hexacyanoferrate(II) ([Fe(CN)6]4-): ≤1 mg/kg, iodide (I-): ≤10 mg/kg, phosphate (PO43-): ≤5 mg/kg, sulfate (SO42-): ≤200 mg/kg

cation traces

Al: ≤5 mg/kg, As: ≤0.1 mg/kg, Ba: ≤5 mg/kg, Bi: ≤5 mg/kg, Ca: ≤10 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤1 mg/kg, K: ≤50 mg/kg, Li: ≤5 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Mo: ≤5 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Sr: ≤5 mg/kg, Zn: ≤5 mg/kg

λ

1 M in H2O

UV absorption

λ: 260 nm Amax: ≤0.01, λ: 280 nm Amax: ≤0.01

suitability

suitable for component for culture media

Quality Level

application(s)

genomic analysis

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此商品
S765371386PHR1321
form

solid

form

powder

form

liquid

form

-

assay

≥99.5% (AT)

assay

≥99.5% (AT)

assay

-

assay

-

solubility

H2O: 1 M at 20 °C, clear, colorless

solubility

H2O: 1 M, clear, colorless

solubility

water: soluble

solubility

-

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

300

grade

for molecular biology

grade

-

grade

for molecular biology

grade

certified reference material, pharmaceutical secondary standard

cation traces

Al: ≤5 mg/kg, Ba: ≤5 mg/kg, Ca: ≤10 mg/kg, Co: ≤5 mg/kg, Cu: ≤5 mg/kg, K: ≤50 mg/kg, Mg: ≤5 mg/kg, Mo: ≤5 mg/kg, Pb: ≤5 mg/kg, Zn: ≤5 mg/kg, As: ≤0.1 mg/kg, Cd: ≤5 mg/kg, Fe: ≤1 mg/kg, Mn: ≤5 mg/kg, Sr: ≤5 mg/kg, Bi: ≤5 mg/kg, Li: ≤5 mg/kg, Cr: ≤5 mg/kg, Ni: ≤5 mg/kg

cation traces

Al: ≤0.0005%, As: ≤0.0001%, Ba: ≤0.0005%, Bi: ≤0.0005%, Ca: ≤0.002%, Cd: ≤0.0005%, Co: ≤0.0005%, Cr: ≤0.0005%, Cu: ≤0.0005%, Fe: ≤0.0001%, K: ≤0.005%, Li: ≤0.0005%, Mg: ≤0.0005%, Mn: ≤0.0005%, Mo: ≤0.0005%, Ni: ≤0.0005%, Pb: ≤0.0005%, Sr: ≤0.0005%, Zn: ≤0.0005%

cation traces

Al: ≤1 mg/kg, Ba: ≤1 mg/kg, Bi: ≤1 mg/kg, Ca: ≤5 mg/kg, Cd: ≤1 mg/kg, Co: ≤1 mg/kg, Cr: ≤1 mg/kg, Cu: ≤1 mg/kg, Fe: ≤1 mg/kg, K: ≤20 mg/kg, Li: ≤1 mg/kg, Mg: ≤1 mg/kg, Mn: ≤1 mg/kg, Mo: ≤1 mg/kg, Ni: ≤1 mg/kg, Pb: ≤1 mg/kg, Sr: ≤1 mg/kg, Zn: ≤1 mg/kg

cation traces

-

General description

氯化钠是一种重要的营养物质,在生物化学和分子生物学中起关键作用,为各种生物化学应用做出了重要贡献。在生物系统中,它作为一种基本的电解质,可调节渗透压、pH平衡和电导率,所有这些都是正常细胞功能和重要生理过程所必需的。此外,氯化钠在维持蛋白质结构和促进酶活性方面起关键作用,对稳定蛋白质构象和催化生化反应至关重要。它还可以调节细胞稳态和促进离子和营养物质跨细胞膜运输。它是PBS和SSC等缓冲液的组成部分,协助各种生化过程,包括密度梯度的产生和生物功能的微调。值得注意的是,氯化钠用于增加缓冲液和培养基中的离子强度,影响特定实验和细胞培养的重要离子环境。此外,它适用于从含有SDS的样品中提取DNA,以及在质粒DNA制备过程中去除小核酸片段。它还可以应用于细胞培养,在细菌、酵母和细胞培养培养基中起重要作用。在分子生物学和细胞培养中,氯化钠是一种不可缺少的盐,在研究的各个方面都举足轻重。它是细胞培养基、裂解缓冲液和蛋白质储存溶液中的常见成分,有助于维持细胞生长和实验的最佳条件。在这些应用中使用的氯化钠具有高纯度,经过严格的杂质测试,如DNases、RNase、磷酸酶、蛋白酶和微量金属,因此是分子生物学和生化研究的理想选择

Application

氯化钠已用于:

  • 作为缓冲溶液组分,制备开路电位法和循环伏安法的pH溶液[1]
  • 制备Bolds基础培养基(BBM)[2]
  • 作为转录和退火缓冲的组分[3]
  • 维持渗透平衡,确保分离的细胞外囊泡(EV)的纯度和稳定性[4]
  • 在制造横向流动装置时,作为横向流动条层合的缓冲层[5]

Biochem/physiol Actions

氯化钠有助于激活辅助性T细胞17(TH17)细胞的稳定诱导。[6]

Features and Benefits

  • 适用于分子生物学和生物化学研究
  • 不含DNase、RNase、NICKase和蛋白酶
  • 经过测试,确认低水平的重金属污染,确保各种应用的适用性
  • 检测痕量阴离子和阳离子

Other Notes

可能会涉及到销售限制
大规模苯酚提取RNA的裂解缓冲液和提取缓冲液的组分[7];从人类有核细胞中提取DNA的简单盐析程序[8]
如需了解生化试剂系列的更多信息,请填写此表
该产品仅用于研究目的,不适合人类食用。

存储类别

13 - Non Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Axenic Cultivation of Mycelium of the Lichenized Fungus, Lobaria pulmonaria (Peltigerales, Ascomycota)
Cornejo C, et al.
Bio-protocol (2015)
D.M. Wallace
Methods in Enzymology, 152, 39-39 (1987)
Sodium chloride drives autoimmune disease by the induction of pathogenic T H 17 cells
Kleinewietfeld M, et al.
Nature, 496(7446), 518-518 (2013)
A simple salting out procedure for extracting DNA from human nucleated cells.
S A Miller et al.
Nucleic acids research, 16(3), 1215-1215 (1988-02-11)
A solid-state thin-film Ag/AgCl reference electrode coated with graphene oxide and its use in a pH sensor
Kim T, et al.
Sensors, 15(3), 6469-6482 (2015)

相关内容

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  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

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    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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