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83264

Sigma-Aldrich

HEPES缓冲溶液

1 M in H2O

Synonym(s):
羟乙基哌嗪乙硫磺酸 溶液, N-(2-羟乙基)哌嗪-N′-(2-乙磺酸)
CAS Number:
MDL编号:
eCl@ss:
32129211
PubChem化学物质编号:
NACRES:
NA.25

形式

liquid

质量水平

100

浓度

1 M in H2O
238 g/L HEPES in H2O

杂质

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

pH值(酸碱度)

5.5±0.5

useful pH range

6.8-8.2

密度

1.07 g/mL at 20 °C

application(s)

diagnostic assay manufacturing

SMILES string

OCCN1CCN(CCS(=O)(O)=O)CC1

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

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一般描述

HEPES被认为是生物学研究中最好的通用缓冲液之一。在生物pH值下,该分子为两性离子,缓冲液pH在 6.8到8.2时有效。HEPES已被广泛应用于一系列应用中,包括组织培养。常用来缓冲空气中的细胞培养基。HEPES在Mg的 体外 实验中得到了应用。

应用

HEPES已可用于:
  • 作为SAPI培养基的组成部分,用于培养 斑疹伤寒菌 疫苗
  • 制备PC12细胞分化培养基。它还是RPMI培养基的一个组份
  • 作为培养大鼠皮层神经元分离缓冲液的组成部分
  • 与MgCl2、KCl、和低渗缓冲液中的精氨酸一起,应用mGRASP法检测哺乳动物突触
  • 作为琼脂糖凝胶制备中所用的Danieu′s aquarium water的组份

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(F)

Not applicable

闪点(C)

Not applicable

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificate of Analysis

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Certificate of Origin

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