所有图片(1)

93443

Sigma-Aldrich

Triton X-100 溶液

BioUltra, for molecular biology, ~10% in H2O

别名:
4-(1,1,3,3-四甲基丁基)苯基-聚乙二醇, Triton X-100, 4-(1,1,3,3-四甲基丁基)苯基-聚乙二醇溶液, 叔辛基苯氧基聚乙烯乙氧基乙醇, 聚乙二醇叔辛基苯基醚
线性分子式:
t-Oct-C6H4-(OCH2CH2)xOH, x= 9-10
CAS号:
Beilstein:
2315025
MDL编号:
PubChem化学物质编号:

质量水平

200

等级

for molecular biology

描述

non-ionic

产品线

BioUltra

分子量

micellar avg mol wt 80,000
average mol wt 625

浓度

~10% in H2O

聚集数

100-155

杂质

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

折射率

n20/D 1.349±0.002

pH

6.5-8.5 (25 °C)

CMC

0.2-0.9 mM (20-25°C)
0.23 mM

转变温度

cloud point 65 °C
pour point ~7 °C

密度

1.01 g/mL at 20 °C

痕量阳离子

Al: ≤1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤100 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
Na: ≤100 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg

吸光度

cut-off at 310 nm

HLB

13.5

SMILES string

CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1

InChI

1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3

InChI key

HNLXNOZHXNSSPN-UHFFFAOYSA-N

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相关类别

一般描述

Triton X-100是一种常用的非离子型的表面活性剂和乳化剂,经常用于生化应用以溶解蛋白质。它被视为一种不变性的相对温和的去污剂,并且在许多参考文献中作为一种常规被添加的试剂而被报道。它用于裂解细胞以提取蛋白和细胞器。还可通透活细胞膜用于转染。

应用

Trion-X可以用于:
  • 非直接免疫荧光和流式细胞仪前细胞渗透
  • 作为细胞同质化裂解缓冲液组分
  • 作为免疫荧光染色遮蔽缓冲液组分
  • 在双荧光染色中制备细胞

生化/生理作用

广泛使用的非离子表面活性剂,用于在温和的非变性条件下进行膜恢复。

法律信息

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

象形图

CorrosionEnvironment

警示用语:

Danger

危险声明

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK Germany

WGK 2

闪点(F)

483.8 °F - closed cup

闪点(C)

251 °C - closed cup

个人防护装备

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

分析证书

原产地证书 (CofO)

Melittin exerts an antitumor effect on non small cell lung cancer cells.
Zhang S F and Chen Z
Molecular Medicine Reports, 16(3), 3581-3586 (2017)
Oxysterols in adipose tissue-derived mesenchymal stem cell proliferation and death.
Silva S F, et al.
The Journal of Steroid Biochemistry and Molecular Biology, 169, 164-175 (2017)
The effect of artificial shrinkage and assisted hatching on the development of mouse blastocysts and cell number after vitrification.
Kim H J, et al.
Clinical and Experimental Reproductive Medicine, 42(3), 94-100 (2015)
Creation of an injectable in situ gelling native extracellular matrix for nucleus pulposus tissue engineering.
Wachs R A, et al.
The Spine Journal : Official Journal of the North American Spine Society, 17(3), 435-444 (2017)
Alena Balashova et al.
Frontiers in neuroscience, 13, 1033-1033 (2019-10-22)
It is well-known that hyaluronic acid (HA) as a component of brain extracellular matrix (ECM) plays a pivotal role in the nervous system and is involved in synaptic plasticity changes in vascular cognitive impairment and dementia. HA breakdown is a...

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