所有图片(4)

A2929

Sigma-Aldrich

琼脂糖

For pulsed field electrophoresis running gel

CAS号:
EC 号:
MDL编号:
PubChem化学物质编号:

生物来源

algae (red)

质量水平

200

形式

powder

应用

electrophoresis: suitable

杂质

≤10% water

EEO

≤0.10

转变温度

gel point 38-43 °C (10% gel)

凝胶强度

≥2000 g/cm2

痕量阴离子

sulfate (SO42-): ≤0.20%

异质活性

DNase, RNase, NICKase, none detected

InChI

1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1

InChI key

MJQHZNBUODTQTK-WKGBVCLCSA-N

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相关类别

特异性

适用于分离高分子量的 DNA。与常规琼脂糖相比,倒转电场电泳使得凝胶更容易处理,分离更快速,高分子量 DNA 的分离度更好。

包装

25, 100, 250 g in poly bottle

应用

专为脉冲场凝胶电泳 (PFGE) 或传统电泳快速分离较大 DNA 片段 (10-2000kb) 而配制。脉冲场凝胶电泳法于 1984 年建立,用于处理大小超出分辨率界限且以相同速率迁移的较大 DNA 片段爬行。在凝胶周围施加了交替脉冲、正交定向的电场。每次电场方向改变,较大的 DNA 片段需要沿着新电场重新定向,否则无法继续迁移。重新定向所需时间取决于 DNA 的大小,因此 DNA 根据大小被分离。

特点和优势

  • 低电渗可以升高电压,使 DNA 迁移更快速。
  • 凝胶强度更高,使得低浓度凝胶稳定性更好,从而导致 DNA 迁移更快。
  • 使用了溴化乙锭染料,因此凝胶具有低背景荧光。

分析说明

以下是与我们的琼脂糖相关的属性列表:
硫酸盐含量 - 用作一种纯度的指标,因为硫酸盐是存在的主要离子基团。
凝胶强度 - 施加在凝胶上让其破裂所必需的力。
凝胶点 - 琼脂糖水溶液在冷却时形成凝胶的温度。 琼脂糖溶液在液体-凝胶转变的过程中会表现出滞后现象 - 也就是说,它们的凝胶点与它们的熔点温度不同。
电内渗(EEO)- 液体通过凝胶的一种运动。琼脂糖凝胶中的阴离子基团被固定在基质上不能移动,但可解离的抗衡阳离子可以向基质中的阴极迁移,从而产生EEO。 由于生物聚合物的电泳运动通常是朝向阳极的,因此EEO会因为内部的对流而破坏分离。

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

分析证书

原产地证书 (CofO)

Sifang Liao et al.
Frontiers in endocrinology, 11, 180-180 (2020-05-07)
The insulin/IGF-signaling pathway is central in control of nutrient-dependent growth during development, and in adult physiology and longevity. Eight insulin-like peptides (DILP1-8) have been identified in Drosophila, and several of these are known to regulate growth, metabolism, reproduction, stress responses...
Yu-Hsuan Cheng et al.
BMC biology, 18(1), 180-180 (2020-12-01)
Ciliates are an ancient and diverse eukaryotic group found in various environments. A unique feature of ciliates is their nuclear dimorphism, by which two types of nuclei, the diploid germline micronucleus (MIC) and polyploidy somatic macronucleus (MAC), are present in...
Michael J Lafferty et al.
The Journal of biological chemistry, 288(40), 28524-28534 (2013-08-21)
Elevated triglycerides are associated with an increased risk of cardiovascular disease, and lipoprotein lipase (LPL) is the rate-limiting enzyme for the hydrolysis of triglycerides from circulating lipoproteins. The N-terminal domain of angiopoietin-like protein 4 (ANGPTL4) inhibits LPL activity. ANGPTL4 was...
Julia Dahlmann et al.
Biomaterials, 34(10), 2463-2471 (2013-01-22)
In most pluripotent stem cell differentiation protocols the formation of embryoid bodies (EBs) is an important step. Here we describe a rapid, straightforward soft lithography approach for the preparation of hydrophilic silicon masters from different templates and the subsequent production...
Sujith V W Weerasinghe et al.
Journal of medicinal chemistry, 51(18), 5542-5551 (2008-08-30)
Histone deacetylase 1 (HDAC1) has been linked to cell growth and cell cycle regulation, which makes it a widely recognized target for anticancer drugs. Whereas variations of the metal-binding and capping groups of HDAC inhibitors have been studied extensively, the...

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