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A9539

Sigma-Aldrich

琼脂糖

BioReagent, for molecular biology, low EEO

CAS号:
EC 号:
MDL编号:
PubChem化学物质编号:
NACRES:
NA.25

质量水平

200

生物来源

algae (marine)

等级

for molecular biology

产品线

BioReagent

形式

powder

应用

electrophoresis: suitable

杂质

≤10% moisture content

EEO

0.09-0.13

转变温度

gel point 36 °C ±1.5 °C (1.5% gel)

凝胶强度

≥1200 g/cm2 (1% gel)

痕量阴离子

sulfate (SO42-): ≤0.15%

异质活性

DNase, RNase, none detected

InChI

1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1

InChI key

MJQHZNBUODTQTK-WKGBVCLCSA-N

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相关类别

包装

10, 25, 50, 100, 250, 500 g in poly bottle

应用

常规琼脂糖是通过凝胶电泳或印迹(Northern 或 Southern)进行日常核酸分析的理想选择,还适用于蛋白质应用,例如 Ouchterlony 免疫扩散和放射状免疫扩散 (RID)。含少量溴化乙锭和 SYBR Green 背景染料。

分析说明

以下是与我们的琼脂糖相关的性质列表:
  • 硫酸盐含量 - 用作纯度指标,因为硫酸盐是其中存在的主要离子基团。
  • 凝胶强度 - 破裂凝胶所需施加的力。
  • 胶凝点 - 琼脂糖水溶液在冷却时的胶凝温度。琼脂糖溶液在液体-凝胶转变的过程中会表现出滞后现象 - 也就是说,它们的凝胶点与它们的熔点温度不同。
  • 电内渗 (EEO) - 液体通过凝胶的一种运动形式。琼脂糖凝胶中的阴离子基团被固定在基质上不能移动,但可解离的对应阳离子可向基质中的阴极迁移,从而产生EEO。由于生物聚合物的电泳运动通常是朝向阳极的,因此EEO会因为内部的对流而破坏分离。
以下是与我们的琼脂糖相关的属性列表:
硫酸盐含量 - 用作一种纯度的指标,因为硫酸盐是存在的主要离子基团。
凝胶强度 - 施加在凝胶上让其破裂所必需的力。
凝胶点 - 琼脂糖水溶液在冷却时形成凝胶的温度。 琼脂糖溶液在液体-凝胶转变的过程中会表现出滞后现象 - 也就是说,它们的凝胶点与它们的熔点温度不同。
电内渗(EEO)- 液体通过凝胶的一种运动。琼脂糖凝胶中的阴离子基团被固定在基质上不能移动,但可解离的抗衡阳离子可以向基质中的阴极迁移,从而产生EEO。 由于生物聚合物的电泳运动通常是朝向阳极的,因此EEO会因为内部的对流而破坏分离。

Storage Class Code

11 - Combustible Solids

WGK Germany

WGK 1

闪点(F)

Not applicable

闪点(C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

分析证书

原产地证书 (CofO)

Stephen AM and Phillips GO
Food Sci. Technol., 160 (2006)
Lukas Kain et al.
Journal of the mechanical behavior of biomedical materials, 85, 225-236 (2018-06-23)
Mechanical assessment of biological materials and tissue-engineered scaffolds is increasingly focusing at lower length scale levels. Amongst other techniques, atomic force microscopy (AFM) has gained popularity as an instrument to interrogate material properties, such as the indentation modulus, at the...
Zita Olah et al.
Ecotoxicology and environmental safety, 144, 115-122 (2017-06-13)
Rotifers have been used in biological research as well-characterized models of aging. Their multi-organ characters and their sensitivity for chemicals and environmental changes make them useful as in vivo toxicological and lifespan models. Our aim was to create a bdelloid...
G Palazzolo et al.
Scientific reports, 7(1), 8499-8499 (2017-08-19)
The need for in vitro models that mimic the human brain to replace animal testing and allow high-throughput screening has driven scientists to develop new tools that reproduce tissue-like features on a chip. Three-dimensional (3D) in vitro cultures are emerging...
Lea I Mikkola et al.
PLoS genetics, 15(7), e1008197-e1008197 (2019-07-20)
Canine hip dysplasia is a common, non-congenital, complex and hereditary disorder. It can inflict severe pain via secondary osteoarthritis and lead to euthanasia. An analogous disorder exists in humans. The genetic background of hip dysplasia in both species has remained...

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