This product has not been tested for use in PCR. The formulation for this product is proprietary however, this is a nondenaturing preparation that does contain zwitterionic detergents. Detergents may interfere with PCR at high concentrations.
B7435
CelLytic ™ B 细胞裂解试剂
For bacterial cell lysis, standard strength
别名:
细胞裂解试剂
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| 规格/货号 | 是否有货 | 价格 |
|---|---|---|
50 mL | 请联系客服了解存货情况 | $167.00 |
500 mL | 请联系客服了解存货情况 | $847.00 |
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$167.00
请联系客服了解存货情况
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Quality Segment
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solution
Application
CelLytic ™ B 细胞裂解试剂已用于细胞裂解，用于提取辅酶 Q10、 重组 Src 激酶相关磷蛋白 1-谷胱甘肽 S -转移酶 (GST) 融合蛋白 和人 α-突触核蛋白。
两性离子洗涤剂的专利、非变性配方，用于细菌细胞裂解和重组蛋白提取。
Features and Benefits
- 比传统方法如超声法和溶菌酶法提取蛋白质效率高
- 可放大 1-25 克细菌细胞浆
- 不干扰下游应用，如亲和层析、IP 和免疫印迹
- 与蛋白酶抑制剂、抑制剂混合物、离液剂、盐、螯合剂、还原剂相容
Other Notes
在 40 mM Trizma ® HCl (pH 8.0) 中配制的洗涤剂混合物。
Legal Information
此产品受美国专利号7,282,475 B2管辖，仅针对研究用途出售。如需商业化使用，需另行征得许可。
CelLytic is a trademark of Sigma-Aldrich Co. LLC
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
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signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2
存储类别
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
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Can i use this product for PCR?
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Can I use CelLytic B with the maltose binding protein system (MBP System)?
1 answer-
CelLytic B cannot be used with the MBP system. We have used this in the maltose binding protein system, and know that it will NOT work in this system, since any and all detergents will destroy binding of protein to the resin.
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What bacterial cells can be used with CelLytic B?
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CelLytic B Reagent by itself (no lysozyme or benzonase) was optimized for E. coli strain BL21. It also works with other common E. coli strains such as DH5?, JM109, and HB 101. The CelLytic B Reagent may be used on other similar bacterial cells. The CelLytic B Plus Kit is compatible with many more difficult to lyse Gram positive bacteria such as Bacillus and Streptococcus.
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When using the CelLytic™ B lysis reagent, do I need to make a dilution prior to measuring protein by Bradford assay?
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When using the CelLytic™ B lysis reagent (B7435, B7310, C8740, and contained in kits CB0050 and CB0500), it is best to use a 1:10 dilution in order to reduce the risk of interference with the Bradford Reagent. A 1:20 dilution would be even better, as long as the protein level remains within the standard curve.
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What is the difference between CelLytic B (Product Nos. B7435, B7310, C8740) and CelLytic B Plus (Product Nos. CB0050 and CB0500)?
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CelLytic B Plus contains CelLytic B, Product No. B7435 as well as lysozyme, benzonase, and a protease inhibitor cocktail for a complete reagent for cell lysates for protein isolation.
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Can I concentrate my sample that was made using CelLytic B?
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If concentrating a sample, be aware that concentration using filter concentrators may increase the detergent concentration significantly and cause issues in downstream applications. heoretically, ways to get around this are TCA precipitation, or diluting the extract below the CMC prior to concentration. This would be at least a 1/5 dilution, based on the following reference: Saito, S., and Tsuchiya, T., Characteristics of n-octyl beta-D-thioglucopyranoside, a new non-ionic detergent useful for membrane biochemistry. Biochem. J., 222(3), 829-832, 1984.
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What is the difference between CelLytic B (Product No. B7435), CelLytic B 2x (Product No. B7310) and CelLytic B 10x (Product No. C8740)?
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CelLytic B 2X has double the strength of CelLytic B for small volume extractions. This allows CelLytic B 2X to be used when a higher protein concentration or lower volume is required. The original formula, CelLytic B, requires 10-20 ml of the reagent for 1 gram of wet cell paste. Only 5 ml of the CelLytic B 2X reagent is required to lyse and extract protein from 1 gram of wet cell paste. CelLytic B 10X is provided as a concentrated mixture of the proprietary detergents found in CelLytic B, without any buffering component. This allows the user to customize the extraction reagent by choosing the detergent concentration and buffering components ideal for the extraction and purification of the protein(s) of interest. CelLytic B 10X is also suitable for the lysis of yeast and mammalian cells.
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Can I obtain protein from inclusion bodies with CelLytic B?
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If the protein is found in inclusion bodies, you need to use an additional reagent, Product No. C5236, CelLytic IB to solubilize the proteins from the inclusion bodies. CelLytic B is used to isolate the inclusion bodies from the bacteria. CelLytic IB, Product No. C5236 can then be used to solubilize the proteins from the inclusion bodies.
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Can I use the CelLytic™ B cell lysate in an activity assay?
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CelLytic B contains a zwitterionic detergent so the protein is active and can be used in protein activity assays.
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Can I use CelLytic B for eukaryotic cells?
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CelLytic B was optimized for bacterial cells. It may lyse some mammalian cells. CelLytic B 10X is a better choice for the lysis of yeast and mammalian cells due to the increased detergent concentration.
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