Merck

C8546

Sigma-Aldrich

纤维素酶 来源于里氏木霉 ATCC 26921

greener alternative

lyophilized powder, ≥1 unit/mg solid

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别名:
1,4-(1,3:1,4) -β- D -葡聚糖 4-葡聚糖-水解酶
CAS号:
EC 号:
MDL编号:

形式

lyophilized powder

specific activity

≥1 unit/mg solid

环保替代产品特性

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

溶解性

deionized water: soluble 5.0 mg/mL (Sterile; In the presence of 0.15% polyhexamethylene biguanide (PHMB).)

环保替代产品分类

储存温度

2-8°C

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此商品
C2730C1794C0615
specific activity

≥1 unit/mg solid

specific activity

≥700 units/g

specific activity

3-10 units/mg solid

specific activity

≥5,000 units/g solid

solubility

deionized water: soluble 5.0 mg/mL (Sterile; In the presence of 0.15% polyhexamethylene biguanide (PHMB).)

solubility

-

solubility

-

solubility

deionized water: soluble 3.0 mg/mL (Sterile; In the presence of 0.15% polyhexamethylene biguanide (PHMB))

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

greener alternative product characteristics

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

一般描述

我们致力于为您带来更加绿色的替代产品,这些产品遵守一项或多项绿色化学12项原则。当用于纤维素乙醇研究时,该产品经优化可提高能效和防止浪费。更多信息请参阅BiofilesEnzymes for Alternative Energy Research上的文章。

应用

Sigma 公司的纤维素酶已用于将纤维寡糖降解为葡萄糖;研究生物可吸收细菌纤维素 (BBC) 材料的生物降解性。
来自里氏木霉的纤维素酶已被用于研究,以评估纤维素酶转录因子 XYR1、ACE2 和 ACE 的差异调节。木霉 (Trichoderma reesei) 产纤维素酶也被用于研究水热预处理麦秸在高固含量下的液化。

生化/生理作用

纤维素酶是将纤维素水解成葡萄糖的酶。纤维素酶用于研究洗涤剂、制药、烘焙和酶制剂生产行业中职业性哮喘的发展。在洗涤剂中加入纤维素酶以改善清洁属性。也可应用于纸制品的生物降解,如纸浆纸、过滤器纸、报纸、办公用纸、微晶纤维素等。

单位定义

一个单位将在 pH 5.0 的 37℃ 下在 1 小时内从纤维素中释放出 1.0μmole 的葡萄糖(孵育时间为2小时)。

制备说明

存在浓度为 5 mg/mL 的 0.15% 聚六亚甲基双胍 (PHMB) 时,溶于无菌去离子 (DI) 水中。

象形图

Health hazard

警示用语:

Danger

危险声明

危险分类

Resp. Sens. 1

储存分类代码

11 - Combustible Solids

WGK

WGK 1

闪点(F)

Not applicable

闪点(C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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Nóra Szijártó et al.
Bioresource technology, 102(2), 1968-1974 (2010-10-05)
Enzymatic liquefaction was studied by measuring continuously the flowability change of high-solids lignocellulose substrates using a real time viscometric method. Hydrolysis experiments of hydrothermally pretreated wheat straw were carried out with purified enzymes from Trichoderma reesei; Cel7A, Cel6A, Cel7B, Cel5A
J P H van Wyk et al.
Bioresource technology, 86(1), 21-23 (2002-11-08)
Environmental issues such as the depletion of non-renewable energy resources and pollution are topical. The extent of solid waste production is of global concern and development of its bioenergy potential can combine issues such as pollution control and bioproduct development
Thomas Portnoy et al.
Eukaryotic cell, 10(2), 262-271 (2010-12-21)
Due to its capacity to produce large amounts of cellulases, Trichoderma reesei is increasingly being investigated for second-generation biofuel production from lignocellulosic biomass. The induction mechanisms of T. reesei cellulases have been described recently, but the regulation of the genes
Ming-Li Yong et al.
Microscopy research and technique, 79(9), 838-844 (2016-07-01)
Rice false smut, caused by the fungal pathogen Villosiclava virens, is one of the most important rice diseases in the world. Previous studies reported that the pathogen has less number of cell wall-degraded genes and attacks dominantly rice stamen filaments
Marko Riedel et al.
The Journal of eukaryotic microbiology, 56(2), 130-135 (2009-05-22)
Transgenic Phytophthora ramorum strains that produce green fluorescent protein (GFP) constitutively were obtained after stable DNA integration using a polyethylene glycol and CaCl₂-based transformation protocol. Green fluorescent protein production was studied in developing colonies and in different propagules of the

实验方案

Enzymatic Assay of Cellulase

To standardize an enzymatic assay procedure of cellulase.

纤维素酶的酶学测定

该实验方案使用分光光度法测定纤维素酶活性。黑曲霉纤维素酶催化内-1,4-β-D-糖苷键的水解。

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