The Imprint Ultra Chromatin IP Kit is Sigma′s second generation chromatin immunoprecipitation (ChIP) kit developed for maximum sensitivity and optimum next-generation sequencing results. It provides a complete solution for Chromatin Immunoprecipitation, including columns and reagents for DNA purification. The kit allows researchers to explore the genome-wide binding sites of low abundance transcription factors (TFs), as well as novel histone modifications. It is optimized for ChIP reactions with chromatin from 106 cells (up to ~50 μg DNA), and can also be scaled up (or several preparations pooled) to accommodate 108 cells for genome-wide binding studies in ChIP-chip and ChIP-Seq applications.
Suitable for downstream applications
Individual target characterization to genome-wide profiling techniques
Characterization of signal transduction pathways
Verification of ChIp-chIP and ChIP-seq data
Use successfully CHiP′ed DNA associated with low abundance, medium, and highly expressed transcription factors, as well as histone modifications
Greater capacity - can be used over a wide range of cell numbers ranging from 2-10 × 106
Maximum sensitivity - capable of detecting low abundance transcription factors with as little as 2 × 106 cells
Employs DNA-Blocked "Staph-Seq" for IP (immunoprecipitation), minimizing contaminating Staph A DNA in downstream ChIP-Seq applications.
Imprint is a registered trademark of Sigma-Aldrich Co. LLC
Kit Components Only
Sodium dodecyl sulfate solution, for molecular biology, 10% in 18 megohm water
Monoclonal Anti-POLR2A antibody produced in mouse, clone 1F17, purified immunoglobulin, buffered aqueous solution