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CHP2NC

Sigma-Aldrich

Imprint® Ultra染色质免疫沉淀试剂盒,不含对照品

ChIP kit for maximum sensitivity, compatible with Next-Gen sequencing

质量水平

200

一般描述

Imprint Ultra Chromatin IP 试剂盒是 Sigma′第二代染色质免疫沉淀(ChIP) 试剂盒,旨在实现最高灵敏度和最佳下一代测序结果。它提供了一套完整的染色质免疫沉淀解决方案,包括用于 DNA 纯化的色谱柱和试剂。该试剂盒让研究人员可以探索低丰度转录因子(TF) 的全基因组结合位点,以及新的组蛋白修饰。它针对来自 106 个细胞(最多约 50 μg DNA)的染色质的 ChIP 反应进行了优化,也可以扩大规模(或合并多个制剂)以适用于108 个细胞,用于 ChIP-chip 和 ChIP-Seq 应用中的全基因组结合研究。

应用

  • 适用于下游应用
  • 从单个靶标表征到全基因组分析的技术
  • 信号转导途径的表征
  • ChIp-chIP和ChIP-seq数据的验证

特点和优势

  • 成功采用与低丰度、中等和高表达转录因子以及组蛋白修饰相关的 CHiP′ed DNA。
  • 更大的容量 - 可用于 2-10 × 106 的各种细胞数目范围
  • 最高的灵敏度 - 能够检测低至 2 × 106个细胞的低丰度转录因子
  • 采用 DNA 封闭的 "Staph-Seq" 进行IP(免疫沉淀),最大限度减少下游 ChIP-Seq 应用中的葡萄球菌A DNA 污染。

法律信息

Imprint is a registered trademark of Sigma-Aldrich Co. LLC

Kit Components Only

Product No.
Description

  • Sodium dodecyl sulfate solution, for molecular biology, 10% in 18 megohm water

  • Monoclonal Anti-POLR2A antibody produced in mouse, clone 1F17, purified immunoglobulin, buffered aqueous solution

Kit Components Also Available Separately

Product No.
Description
SDS

  • S6576ChIP Next Gen Seq Sepharose®

  • I8896IGEPAL® CA-630, for molecular biology

  • S5150Sodium chloride solution, 5 M in H2O, BioReagent, for molecular biology, suitable for cell culture

  • W4502Water, Nuclease-Free Water, for Molecular Biology

  • I5006IgG from rabbit serum, reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powder

  • I5381IgG from mouse serum, reagent grade, ≥95% (SDS-PAGE), lyophilized powder

  • A7638Bovine Serum Albumin, lyophilized powder, essentially globulin free, ≥99% (agarose gel electrophoresis)

  • A84564-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride, ≥97.0% (HPLC)

  • D9156Deoxyribonucleic acid, single stranded from salmon testes, For hybridization

  • M7023Anti-Mouse IgG (whole molecule) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution

  • P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution

  • R4642Ribonuclease A from bovine pancreas, (Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)

See All (12)

警示用语:

Danger

危险分类

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Ox. Liq. 1 - Resp. Sens. 1 - Skin Corr. 1B - STOT SE 3

靶器官

Respiratory system

储存分类代码

5.1A - Strongly oxidizing hazardous materials

WGK

WGK 3

Certificate of Analysis

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Certificate of Origin

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