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CHP2NC

Sigma-Aldrich

Imprint® Ultra染色质免疫沉淀试剂盒,不含对照品

ChIP kit for maximum sensitivity, compatible with Next-Gen sequencing

质量水平

200

相关类别

一般描述

The Imprint Ultra Chromatin IP Kit is Sigma′s second generation chromatin immunoprecipitation (ChIP) kit developed for maximum sensitivity and optimum next-generation sequencing results. It provides a complete solution for Chromatin Immunoprecipitation, including columns and reagents for DNA purification. The kit allows researchers to explore the genome-wide binding sites of low abundance transcription factors (TFs), as well as novel histone modifications. It is optimized for ChIP reactions with chromatin from 106 cells (up to ~50 μg DNA), and can also be scaled up (or several preparations pooled) to accommodate 108 cells for genome-wide binding studies in ChIP-chip and ChIP-Seq applications.

应用

  • Suitable for downstream applications
  • Individual target characterization to genome-wide profiling techniques
  • Characterization of signal transduction pathways
  • Verification of ChIp-chIP and ChIP-seq data

特点和优势

  • Use successfully CHiP′ed DNA associated with low abundance, medium, and highly expressed transcription factors, as well as histone modifications
  • Greater capacity - can be used over a wide range of cell numbers ranging from 2-10 × 106
  • Maximum sensitivity - capable of detecting low abundance transcription factors with as little as 2 × 106 cells
  • Employs DNA-Blocked "Staph-Seq" for IP (immunoprecipitation), minimizing contaminating Staph A DNA in downstream ChIP-Seq applications.

法律信息

Imprint is a registered trademark of Sigma-Aldrich Co. LLC

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书

  • S6576ChIP Next Gen Seq Sepharose®

  • I8896IGEPAL® CA-630, for molecular biology

  • Sodium dodecyl sulfate solution, for molecular biology, 10% in 18 megohm water

  • S5150Sodium chloride solution, 5 M in H2O, BioReagent, for molecular biology, suitable for cell culture

  • W4502Water, Nuclease-Free Water, for Molecular Biology

  • I5006IgG from rabbit serum, reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powder

  • I5381IgG from mouse serum, reagent grade, ≥95% (SDS-PAGE), lyophilized powder

  • A7638Bovine Serum Albumin, lyophilized powder, essentially globulin free, ≥99% (agarose gel electrophoresis)

  • A84564-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride, ≥97.0% (HPLC)

  • D9156Deoxyribonucleic acid, single stranded from salmon testes, For hybridization

  • M7023Anti-Mouse IgG (whole molecule) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution

  • Monoclonal Anti-POLR2A antibody produced in mouse, clone 1F17, purified immunoglobulin, buffered aqueous solution

  • P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution

  • R4642Ribonuclease A from bovine pancreas, (Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)

查看所有结果 (14)

警示用语:

Danger

危险分类

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Ox. Liq. 1 - Resp. Sens. 1 - Skin Corr. 1B - STOT SE 3

靶器官

Central nervous system

储存分类代码

5.1A - Strongly oxidizing hazardous materials

WGK

WGK 3

分析证书

原产地证书 (CofO)

Comparison of sample preparation methods for ChIP-chip assays.
O'Geen, H., et al.
Biotechniques, 41, 557-557 (2006)
Philippe Collas
Molecular biotechnology, 45(1), 87-100 (2010-01-16)
The biological significance of interactions of nuclear proteins with DNA in the context of gene expression, cell differentiation, or disease has immensely been enhanced by the advent of chromatin immunoprecipitation (ChIP). ChIP is a technique whereby a protein of interest
Genomic maps of long noncoding RNA occupancy reveal principles of RNA-chromatin interactions.
Chu, C., et al.
Molecular Cell (2011)
Chromatin isolation by RNA purification (ChIRP).
Chu, C., et al.
Journal of Visualized Experiments (2012)
M H Kuo et al.
Methods (San Diego, Calif.), 19(3), 425-433 (1999-12-02)
Chromatin structure plays important roles in regulating many DNA-templated processes, such as transcription, replication, and recombination. Considerable progress has recently been made in the identification of large, multisubunit complexes dedicated to these nuclear processes, all of which occur on nucleosomal

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