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CORET

Sigma-Aldrich

含有Taq DNA聚合酶的PCR核心试剂盒

PCR components in separate tubes to allow for optimization

eCl@ss:
32161000
NACRES:
NA.55

质量水平

200

形式

liquid

用途

sufficient for 250 reactions

特点

dNTPs included
hotstart: no

应用

PCR: suitable

颜色

colorless

input

purified DNA

运输

wet ice

储存温度

−20°C

应用

PCR Core kit with Taq DNA polymerase is suitable for the amplification of genomic DNA, cDNA, or cloned DNA templates. Each component has been tested in the polymerase chain reaction. Optimal concentrations of Taq polymerase, template DNA, primers, and MgCl2 will depend on the specifics of the system. It may be necessary to optimize each component separately, especially the Taq and Mg2+ concentrations and the cycling parameters.

数量

试剂盒中含有足够用于250单位Taq DNA聚合酶的试剂。

其他说明

以下试剂必需但并未提供在试剂盒内:
  • 矿物油,M 8662(可选)
  • 热循环仪
  • 引物
  • 待扩增的DNA
  • 氯仿,C 7559(可选)

法律信息

No license is conveyed with the purchase of this product under any of US Patents Nos. 5,804,375, 5,994,056, 6,171,785, 6,214,979, 5,538,848, 5,723,591, 5,876,930, 6,030,787, and 6,258,569, and corresponding patents outside the United States, or any other patents or patent applications, relating to the 5′ Nuclease and dsDNA-Binding Dye Processes. For further information contact the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书

  • P219210X PCR Buffer, Optimized for routine PCR with MgCl2 included 1 mL/vial

  • P231710X PCR Buffer without MgCl2, Optimized for routine PCR without MgCl2 1 mL/vial

  • M8787Magnesium chloride solution, PCR Reagent, 25 mM MgCI2 solution for PCR 1 mL/vial

  • W1754Water, PCR Reagent 3 vial(s)

  • D7295Deoxynucleotide Mix, 10 mM, Molecular Biology Reagent .25 mL

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable

分析证书

原产地证书 (CofO)

Genetic diversity and structure of the tree Manilkara zapota in a naturally fragmented tropical forest
Martiinez N, et al.
Journal of tropical medicine, 33(4), 285-294 (2017)
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 14-14 (1989)

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