Merck
所有图片(1)

D4545

Sigma-Aldrich

Taq DNA聚合酶 来源于水生栖热菌

with 10× PCR reaction buffer without MgCl2

所有图片(1)
别名:
Taq polymerase, Taq polymerase enzyme
CAS号:
9012-90-2
EC 号:
2.7.7.7 (BRENDA, IUBMB)
MDL编号:
MFCD00166026

重组

expressed in E. coli

形式

liquid

用途

sufficient for 1500 reactions
 sufficient for 250 reactions
 sufficient for 50 reactions
 sufficient for 5000 reactions

特点

dNTPs included: no
hotstart: no

浓度

5 units/μL

technique(s)

PCR: suitable

颜色

colorless

input

purified DNA

适用性

suitable for PCR and automated sequencing reactions

application(s)

agriculture

运输

wet ice

储存温度

−20°C

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一般描述

Taq聚合酶是一种热稳定的DNA聚合酶，以嗜热细菌水生栖热菌命名。 一种稳定的脱氧核糖核酸（DNA）聚合酶（最适温度为80°C）已从极端嗜热菌水生栖热菌中纯化出来。 该酶没有磷酸单酯酶，磷酸二酯酶和单链核酸外切酶活性。 该酶的最大活性需要所有四个脱氧核糖核苷酸和活化的小牛胸腺DNA。

应用

来自水生栖热菌的Taq DNA聚合酶已用于：
  • DNA提取过程（基因扩增和测序过程）
  • 基因分型
  • 聚合酶链式反应(PCR)，研究上皮中性粒细胞活化肽78 (ENA-78)和白细胞介素-8 (IL-8)的组成性产生
  • 通过常规PCR扩增原代内皮细胞的RNA

生化/生理作用

Taq聚合酶可催化寡核苷酸引物驱动的、DNA模板依赖性地将dNTP掺入互补DNA链中。 它具有5′至3′聚合酶和核酸外切酶活性。

特点和优势

  • 在单独的试管中提供MgCl 2，以优化MgCl2。
  • 可以耐受反复加热至95 °C而不出现明显活性损失。

包装

Taq DNA聚合酶具有两种形式，含MgCl2的优化型10倍反应缓冲液(D1806)，或不含MgCl2的10倍反应缓冲液加独立管装MgCl2以用于滴定（D4545）。 第二种形式可能是确定最佳扩增条件所必需的。
不含MgCl2的Taq DNA聚合酶10倍反应缓冲液 包含单独一管的25  mM MgCl2

其他说明

Taq DNA聚合酶是一种来自于嗜热菌水生栖热菌（ Thermus aquaticus）的特殊热稳定酶。 该酶的重组形式可在大肠杆菌（ E. coli）中表达。 经过SDS-PAGE分析显示，这种分子量为94kDa的蛋白质不含可检测水平的污染性核酸内切酶或核酸外切酶。 它具有5′→3′聚合酶和核酸外切酶活性。

单位定义

一个酶活性单位是指在74℃下在30分钟内将10 nmol总dNTP掺入酸性可沉淀DNA中所需的酶量。

法律信息

本产品的使用受到如下一项或多项美国专利及其对应的境外专利声明保护：US 8,404,464和US 7,972,828。 购买本产品，即相当于依照境外的专利声明获得了一份受限制、不可转让的使用许可。

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(F)

Not applicable

闪点(C)

Not applicable

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原产地证书 (CofO)

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Quotes and Ordering

Bulk Quote-Order Product
A Chien et al.
Journal of bacteriology, 127(3), 1550-1557 (1976-09-01)
A stable deoxyribonucleic acid (DNA) polymerase (EC 2.7.7.7) with a temperature optimum of 80 degrees C has been purified from the extreme thermophile Thermus aquaticus. The enzyme is free from phosphomonoesterase, phosphodiesterase and single-stranded exonuclease activities. Maximal activity of the
Nick A Bersinger et al.
Fertility and sterility, 89(5 Suppl), 1530-1536 (2007-09-01)
To analyze the constitutive production of epithelial neutrophil activating peptide 78 (ENA-78) and interleukin-8 (IL-8) by epithelial cells and the response of these cells to cytokine stimulation. In vitro study using eutopic endometrial tissue. University hospital. Cycling women undergoing laparoscopy
Slobodan Jergic et al.
The EMBO journal, 32(9), 1322-1333 (2013-02-26)
Processive DNA synthesis by the αεθ core of the Escherichia coli Pol III replicase requires it to be bound to the β2 clamp via a site in the α polymerase subunit. How the ε proofreading exonuclease subunit influences DNA synthesis
Ryan E Davey et al.
Stem cells (Dayton, Ohio), 24(11), 2538-2548 (2006-07-11)
Highly ordered aggregates of cells, or niches, regulate stem cell fate. Specific tissue location need not be an obligatory requirement for a stem cell niche, particularly during embryogenesis, where cells exist in a dynamic environment. We investigated autoregulatory fixed-location-independent processes
Claire Palles et al.
Nature genetics, 45(2), 136-144 (2012-12-25)
Many individuals with multiple or large colorectal adenomas or early-onset colorectal cancer (CRC) have no detectable germline mutations in the known cancer predisposition genes. Using whole-genome sequencing, supplemented by linkage and association analysis, we identified specific heterozygous POLE or POLD1
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