Merck
All Photos(9)

D8130

Sigma-Aldrich

5,5'-二硫代双(2-硝基苯甲酸)

greener alternative

≥98%, BioReagent, suitable for determination of sulfhydryl groups

Synonym(s):
6,6′-二硝基-3,3′-二硫代苯甲酸, DTNB, Ellman 试剂, 二硫代硝基苯甲酸, 双(3-羧基-4-硝基苯基)二硫
Linear Formula:
[-SC6H3(NO2)CO2H]2
CAS Number:
Molecular Weight:
396.35
Beilstein:
1896821
EC 号:
MDL编号:
PubChem化学物质编号:
NACRES:
NA.26

产品线

BioReagent

质量水平

200

测定

≥98%

形式

powder

环保替代产品特性

Atom Economy
Design for Energy Efficiency
Use of Renewable Feedstocks
Learn more about the Principles of Green Chemistry.

mp

240-245 °C (dec.) (lit.)

适用性

suitable for determination of sulfhydryl groups

环保替代产品分类

SMILES string

OC(=O)c1cc(SSc2ccc(c(c2)C(O)=O)[N+]([O-])=O)ccc1[N+]([O-])=O

InChI

1S/C14H8N2O8S2/c17-13(18)9-5-7(1-3-11(9)15(21)22)25-26-8-2-4-12(16(23)24)10(6-8)14(19)20/h1-6H,(H,17,18)(H,19,20)

InChI key

KIUMMUBSPKGMOY-UHFFFAOYSA-N

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一般描述

5,5′双二硫代(2-硝基苯甲酸)也称为DTNB或Ellman′s试剂。这是一种与巯基相互作用的水溶性试剂,在中性pH下方便地应用。硫代阴离子(RS-)与Ellman′s试剂(DTNB2-)反应生成2-硝基-5-硫代苯甲酸盐阴离子(TNB2-)和一种混合二硫化物(R-S-TNB-)。TNB2- 具有较高的摩尔吸收系数,在412nm处有吸收。这种吸收与pH无关,在pH>7.3。

应用

Ellman′s试剂可用于乙酰胆碱酯酶的检测,并可对半胱氨酸残基进行特异性标记。该试剂是测定蛋白质、多肽和组织中游离巯基含量的敏感试剂。

包装

500 mg in glass insert
1, 5, 10, 25, 100 g in poly bottle

储存分类代码

11 - Combustible Solids

WGK

WGK 3

个人防护装备

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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