Unfortunately, the kit components are not sold separately.
DUO94004
Duolink® flowPLA 检测试剂盒- FarRed
Duolink® PLA kit for Flow Cytometry with FarRed Detection
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| 包装规格 | 货号 | 是否有货 | 价格 |
|---|---|---|---|
| 40 tests | 请联系客服了解存货情况 | $1,310.00 |
关于此项目
NACRES:
NA.32
UNSPSC Code:
41105331
Fluorescence:
λex 644 nm; λem 669 nm
Technique(s):
flow cytometry: suitable, immunofluorescence: suitable, proximity ligation assay: suitable
product line
Duolink®
technique(s)
flow cytometry: suitable, immunofluorescence: suitable, proximity ligation assay: suitable
fluorescence
λex 644 nm; λem 669 nm
suitability
suitable for fluorescence
shipped in
dry ice
storage temp.
−20°C
General description
Duolink® flowPLA检测试剂盒包含含有荧光团的检测寡核苷酸(lex = 644 nm/lem = 669 nm)。
Application
Duolink® flowPLA检测试剂盒–FarRed已用于原位邻位连接试验：
- 检测细胞维甲酸结合蛋白1 (Crabp1)与快速加速纤维肉瘤(Raf)激酶- MAPK-Erk激酶(Mek)信号通路组分之间的相互作用和复合物形成[1]
- 研究人类培养的MOLT-4细胞和HeLa细胞中的蛋白质相互作用[2]
- 以可视化Beclin-1蛋白与14-3-3t在神经元中的相互作用[3]
- 研究移植内皮细胞中蛋白质的相互作用[4]
基于邻位连接技术（PLA），Duolink® PLA可实现在单分子水平上内源性检测固定细胞中的蛋白质相互作用、翻译后修饰和蛋白表达水平。
Duolink® flowPLA检测试剂盒可通过流式细胞术灵敏地检测细胞群内蛋白质、蛋白质-蛋白质相互作用和蛋白质修饰。Duolink® flowPLA实验需要固定的悬浮细胞、两种可特异性识别目标蛋白的一抗、一对PLA探针（一个PLUS和一个MINUS）、洗涤缓冲液和Duolink® flowPLA检测试剂盒。flowPLA试剂盒具有5种不同荧光染料：紫色、红色、绿色、橙色或远红外。flowPLA试剂盒包含流式细胞术对结合的PLA探针进行扩增和检测所需的所有试剂。使用标准流式细胞术分析设备进行分析。使用者必须提供固定的细胞悬液、一抗和相应的OLA探针。
遵循Duolink® PLA流式细胞术方案使用本产品。
请访问我们的Duolink® PLA流式细胞术页面，了解如何运行Duolink®流式实验、应用程序、故障排除等。
应用说明
需要一抗。采用标准免疫荧光（IF）、免疫组化（IHC）或免疫细胞化学（ICC）测定法检测一抗（IgG类，单克隆或多克隆），确定最佳的固定、封闭和滴度条件。推荐经流式验证的抗体。
请让我们助您完成工作，了解有关我们的定制服务计划的更多信息，加速您的Duolink®项目
查看完整的Duolink®产品列表
Duolink® flowPLA检测试剂盒可通过流式细胞术灵敏地检测细胞群内蛋白质、蛋白质-蛋白质相互作用和蛋白质修饰。Duolink® flowPLA实验需要固定的悬浮细胞、两种可特异性识别目标蛋白的一抗、一对PLA探针（一个PLUS和一个MINUS）、洗涤缓冲液和Duolink® flowPLA检测试剂盒。flowPLA试剂盒具有5种不同荧光染料：紫色、红色、绿色、橙色或远红外。flowPLA试剂盒包含流式细胞术对结合的PLA探针进行扩增和检测所需的所有试剂。使用标准流式细胞术分析设备进行分析。使用者必须提供固定的细胞悬液、一抗和相应的OLA探针。
遵循Duolink® PLA流式细胞术方案使用本产品。
请访问我们的Duolink® PLA流式细胞术页面，了解如何运行Duolink®流式实验、应用程序、故障排除等。
应用说明
需要一抗。采用标准免疫荧光（IF）、免疫组化（IHC）或免疫细胞化学（ICC）测定法检测一抗（IgG类，单克隆或多克隆），确定最佳的固定、封闭和滴度条件。推荐经流式验证的抗体。
请让我们助您完成工作，了解有关我们的定制服务计划的更多信息，加速您的Duolink®项目
查看完整的Duolink®产品列表
Biochem/physiol Actions
远红荧光检测试剂
使用合适的激光器进行λex 644 nm激发
使用合适的滤光片进行λem 669 nm发射
使用合适的激光器进行λex 644 nm激发
使用合适的滤光片进行λem 669 nm发射
Features and Benefits
- 采用流式细胞仪分析蛋白质与蛋白质的相互作用
- 邻近连接法分析细胞群
- 通过滚环扩增提高对低丰度靶标的灵敏度
- 不需要过表达或基因操纵
- 可进行相对量化
- 可与任何流式细胞仪配合使用
- 易于遵循灵活方案
- 结果可直接发表
Other Notes
本品包括以下组分：
有关更多信息，参见数据表。
- 5x检测溶液-FarRed（DUO84004）
- 5x连接缓冲液（DUO82009）
- 5x扩增缓冲液（DUO82050）
- 连接酶（1U/μL）
- 聚合酶（10U/μL）
有关更多信息，参见数据表。
Legal Information
Duolink is a registered trademark of Merck KGaA, Darmstadt, Germany
PLA is a registered trademark of Merck KGaA, Darmstadt, Germany
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此商品 | |||
|---|---|---|---|
| suitability suitable for fluorescence | suitability suitable for fluorescence | suitability suitable for fluorescence | suitability suitable for fluorescence |
| fluorescence λex 644 nm; λem 669 nm | fluorescence λex 594 nm; λem 624 nm | fluorescence λex 554 nm; λem 576 nm | fluorescence λex 495 nm; λem 527 nm |
| technique(s) flow cytometry: suitable, proximity ligation assay: suitable, immunofluorescence: suitable | technique(s) flow cytometry: suitable, immunofluorescence: suitable, proximity ligation assay: suitable | technique(s) flow cytometry: suitable, immunofluorescence: suitable, proximity ligation assay: suitable | technique(s) flow cytometry: suitable, immunofluorescence: suitable, proximity ligation assay: suitable |
| product line Duolink® | product line Duolink® | product line Duolink® | product line Duolink® |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
signalword
Danger
存储类别
10 - Combustible liquids
wgk
WGK 3
hcodes
Hazard Classifications
Aquatic Chronic 2 - ED ENV 1 - Resp. Sens. 1
实验方案
本实验方案描述如何使用Duolink® PLA邻位连接试剂，通过流式细胞术检测细胞群内的单个蛋白质、蛋白修饰和蛋白相互。
商品
了解有关将邻位连接技术 (PLA) 与流式细胞术相结合的新试剂盒的更多信息，实现了蛋白互作流式分析。
Considerations for proper experimental design, preparation and execution of the Duolink® PLA for flow cytometry protocol.
General tips and tricks for proper experiment execution, aid in identifying potential problems, and provide solutions to ensure a successful Duolink® PLA experiment for flow cytometry.
Adriana Cassaro et al.
Hematological oncology, 39(3), 364-379 (2021-01-27)
Wnt/Fzd signaling has been implicated in hematopoietic stem cell maintenance and in acute leukemia establishment. In our previous work, we described a recurrent rearrangement involving the WNT10B locus (WNT10BR ), characterized by the expression of WNT10BIVS1 transcript variant, in acute
Tyler J Burns et al.
Cytometry. Part A : the journal of the International Society for Analytical Cytology, 91(2), 180-189 (2017-01-18)
To quantify visual and spatial information in single cells with a throughput of thousands of cells per second, we developed Subcellular Localization Assay (SLA). This adaptation of Proximity Ligation Assay expands the capabilities of flow cytometry to include data relating
Sofie Selmer Andersen et al.
Cytokine, 64(1), 54-57 (2013-06-04)
Many cytokine receptors are cell surface proteins that promiscuously combine to form active signalling homo- or heterodimers. Thus, receptor chain dimerization can be viewed as a direct measure of a high probability of intracellular signalling by specific cytokines. Proximity ligation
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| DUO94004-40TST | 04061835515844 |
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Is it possible to just buy the 5x Detection Solution separately?
1 answer-
Helpful?
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Can I use this kit in conjunction with staining for other markers? If so, would you recommend staining with the PLA kit first, followed by staining with other primary-conjugated antibodies?
1 answer-
It is possible to counterstain after the Duolink in situ amplification. It is recommended to apply the counterstaining protocol after the completion of the Amplification step in section 7.3, step 5 of the Duolink In Situ Fluorescence User Manual.
Please see the protocol below:
https://www.sigmaaldrich.com/technical-documents/protocol/protein-biology/protein-and-nucleic-acid-interactions/duolink-counterstainHelpful?
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Hello, can I also dilute the 5 x detection stock in something else than high purity water? Maybe PBS?
1 answer-
It is not recommended to use any other solvent to dilute the 5x detection stock due to the sensitivity of this reaction. The Duolink kits have been optimized for use under the conditions described. Deviation from the protocol may not offer the best results. Please see the link below to view the full product information for the Duolink PLAFlow kits:
https://www.sigmaaldrich.com/technical-documents/technical-article/protein-biology/protein-and-nucleic-acid-interactions/duolink-pla-flow-cytometry-kitsHelpful?
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