Merck

F1804

Sigma-Aldrich

单克隆抗-FLAG® M2 小鼠抗

1 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

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别名:
flag抗体, 抗 ddddk, 抗 dykddddk
NACRES:
NA.32

生物来源

mouse

偶联物

unconjugated

抗体形式

affinity isolated antibody

antibody product type

primary antibodies

克隆

M2, monoclonal

形式

buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

纯化方式

affinity chromatography (affinity tag purification)

species reactivity

all

浓度

1 mg/mL

同位素/亚型

IgG1

免疫原序列

DYKDDDDK

运输

wet ice

储存温度

−20°C

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此商品
F2426F3165F9291
conjugate

unconjugated

conjugate

-

conjugate

unconjugated

conjugate

biotin conjugate

antibody form

affinity isolated antibody

antibody form

-

antibody form

purified immunoglobulin (Purified IgG1 subclass)

antibody form

purified immunoglobulin

clone

M2, monoclonal

clone

M2, monoclonal

clone

M2, monoclonal

clone

M2, monoclonal

form

buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

form

-

form

buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)

form

buffered aqueous glycerol solution

species reactivity

all

species reactivity

-

species reactivity

all

species reactivity

all

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一般描述

小鼠抗-FLAG M2抗体,可与含有FLAG肽序列结合的亲和纯化单克隆抗体。该抗体可识别FLAG肽序列的N-末端、Met-N-末端、C-末端和融合蛋白的内部位点。

纯化方法 - 亲和标签纯化

特异性

结合位点:N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C

免疫原

FLAG;肽序列DYKDDDDK

应用

用于通过免疫印迹、免疫沉淀(IP)、免疫组化、免疫荧光及免疫细胞化学进行FLAG融合蛋白的高灵敏度及特异性检测。 优化用于在哺乳动物、植物和细菌表达系统中进行FLAG融合蛋白的单条带检测。

Western Blotting和EIA

通过我们的FLAG®文献端口浏览更多应用参考文献。

外形

在磷酸盐缓冲液中含有50%甘油。

制备说明

在立即使用前用3%脱脂牛奶稀释于Tris缓冲盐溶液(TBS),pH 8.0

法律信息

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

储存分类代码

10 - Combustible liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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商品

Immunoblotting (Western blot transfer) is a common technique in modern proteomics research.

免疫印迹(蛋白质印迹转印)是现代蛋白质组学研究中的常用技术。

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蛋白质与核酸相互作用试剂,以及用于研究蛋白质-RNA、蛋白质-DNA、蛋白质-蛋白质相互作用和相关应用的资源。

Protein and nucleic acid interaction reagents and resources for investing protein-RNA, protein-DNA, and protein-protein interactions and associated applications.

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