Merck

F3165

Sigma-Aldrich

单克隆抗-FLAG® M2 小鼠抗

clone M2, purified immunoglobulin (Purified IgG1 subclass), buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)

登录to View Organizational & Contract Pricing

别名:
抗 ddddk, 抗 dykddddk
NACRES:
NA.32

生物来源

mouse

质量水平

偶联物

unconjugated

抗体形式

purified immunoglobulin (Purified IgG1 subclass)

antibody product type

primary antibodies

克隆

M2, monoclonal

形式

buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)

纯化方式

using Protein A

species reactivity

all

浓度

3.8-4.2 mg/mL

technique(s)

western blot: 10 μg/mL (Protein A)

同位素/亚型

IgG1

免疫原序列

DYKDDDDK

运输

dry ice

储存温度

−20°C

正在寻找类似产品? Visit 产品对比指南

相关类别

比较类似商品

查看完整比较结果

显示差异

1 of 4

此商品
F1804A9469F2426
conjugate

unconjugated

conjugate

unconjugated

conjugate

alkaline phosphatase conjugate

conjugate

-

antibody form

purified immunoglobulin (Purified IgG1 subclass)

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

-

clone

M2, monoclonal

clone

M2, monoclonal

clone

M2, monoclonal

clone

M2, monoclonal

form

buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)

form

buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

form

buffered aqueous glycerol solution

form

-

species reactivity

all

species reactivity

all

species reactivity

all

species reactivity

-

Still not finding the right product?  

Give our Product Selector Tool a try.

一般描述

抗-Flag M2抗体用于检测Flag融合蛋白质。该单克隆产生于小鼠中并可识别FLAG序列的N-末端、Met N-末端和C-末端。该抗体还可在内部位点识别FLAG。不同于M1,M2不是钙依赖性的。
F3165使用蛋白质A树脂进行亲和纯化,因此不仅含有抗-FLAG M2抗体,还有少量的原生小鼠IgG,这会增加它在大多数应用中的敏感性。
纯化方法——蛋白质A

免疫原

FLAG;肽序列DYKDDDDK

应用

小鼠产生的 ANTI-FLAG® M2 单克隆抗体已用于:
  • 免疫印迹
  • 免疫沉淀
  • 免疫细胞化学
  • 免疫荧光
  • ELISA
  • EIA
  • 染色质免疫沉淀
  • 电子显微分析
  • 流式细胞分析
  • 高速迁移测定

浏览我们有关其他应用的参考文献,请访问 FLAG® 文献页面。

制备说明

用3%脱脂牛奶在Tris缓冲盐溶液(TBS),pH 8.0中将抗体从0.5-10 ug/mL进行稀释

储存及稳定性

将稀释后的抗体分装并-20°C储存。不建议反复冻融。
注意:随着时间流逝,由于分子间的疏水相互作用,少量纯化抗体会从溶液中沉淀出来。如果在该产品中观察到沉淀,请短暂离心小瓶以复溶沉淀。从澄清上清液中取出所需体积的抗体溶液备用。这不会改变纯化抗体在蛋白质印迹或免疫沉淀应用中的性能。

法律信息

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

储存分类代码

12 - Non Combustible Liquids

WGK

nwg

闪点(°F)

Not applicable

闪点(°C)

Not applicable


分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

在网站页面上,产品编号会附带包装尺寸/数量一起显示(例如:T1503-25G)。请确保 在“产品编号”字段中仅输入产品编号 (示例: T1503).

示例

T1503
货号
-
25G
包装规格/数量

其它示例:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

输入内容 1.000309185)

遇到问题?欢迎随时联系我们技术服务 寻求帮助

批号可以在产品标签上"批“ (Lot或Batch)字后面找到。

Aldrich 产品

  • 如果您查询到的批号为 TO09019TO 等,请输入去除前两位字母的批号:09019TO。

  • 如果您查询到的批号含有填充代码(例如05427ES-021),请输入去除填充代码-021的批号:05427ES。

  • 如果您查询到的批号含有填充代码(例如 STBB0728K9),请输入去除填充代码K9的批号:STBB0728。

未找到您寻找的产品?

部分情况下,可能未在线提供COA。如果搜索不到COA,可在线索取。

索取COA

其他客户在看

Casey C Fowler et al.
Nature communications, 10(1), 3684-3684 (2019-08-17)
Bacterial toxins with an AB5 architecture consist of an active (A) subunit inserted into a ring-like platform comprised of five delivery (B) subunits. Salmonella Typhi, the cause of typhoid fever, produces an unusual A2B5 toxin known as typhoid toxin. Here
Fangzhi Tan et al.
Nature communications, 10(1), 3733-3733 (2019-08-21)
Hearing loss is the most common sensory disorder. While gene therapy has emerged as a promising treatment of inherited diseases like hearing loss, it is dependent on the identification of gene delivery vectors. Adeno-associated virus (AAV) vector-mediated gene therapy has
Aleksandra Pajak et al.
PLoS genetics, 15(7), e1008240-e1008240 (2019-08-01)
The RNA helicase SUV3 and the polynucleotide phosphorylase PNPase are involved in the degradation of mitochondrial mRNAs but their roles in vivo are not fully understood. Additionally, upstream processes, such as transcript maturation, have been linked to some of these
Jeong Gu Kang et al.
Scientific reports, 9(1), 11960-11960 (2019-08-21)
Despite the increased interest in epigenetic research, its progress has been hampered by a lack of satisfactory tools to control epigenetic factors in specific genomic regions. Until now, many attempts to manipulate DNA methylation have been made using drugs but
Hong Zhu et al.
Molecular biology of the cell, 24(11), 1619-1637 (2013-04-12)
Charcot-Marie-Tooth (CMT) disease is an inherited neurological disorder. Mutations in the small integral membrane protein of the lysosome/late endosome (SIMPLE) account for the rare autosomal-dominant demyelination in CMT1C patients. Understanding the molecular basis of CMT1C pathogenesis is impeded, in part

商品

比较anti-FLAG® M2磁珠的小规模FLAG®标签蛋白纯化的不同洗脱方法。

相关内容

Protein and nucleic acid interaction reagents and resources for investing protein-RNA, protein-DNA, and protein-protein interactions and associated applications.

蛋白质与核酸相互作用试剂,以及用于研究蛋白质-RNA、蛋白质-DNA、蛋白质-蛋白质相互作用和相关应用的资源。

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门