Merck
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F4799

Sigma-Aldrich

3X FLAG®

lyophilized powder

Synonym(s):
dykddddk肽, ddddk肽, Met-Asp-Tyr-Lys-Asp-His-Asp-Gly-Asp-Tyr-Lys-Asp-His-Asp-Ile-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys
Empirical Formula (Hill Notation):
C120H169N31O49S
Molecular Weight:
2861.87
MDL编号:
NACRES:
NA.32

形式

lyophilized powder

质量水平

200

浓度

(Recommended working concentration is 100 μg/ml for elute 3X FLAG fusion proteins from the ANTI-FLAG® M2 affinity gel.)

运输

wet ice

储存温度

2-8°C

一般描述

3X FLAG肽是一种具有23个氨基酸残基的合成肽。在该肽中Asp-Tyr-Lys-Xaa-Xaa-Asp基序重复了三次。在C-末端的八个氨基酸组成了经典的FLAG序列(Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys)。

应用

用于从在溶液中的或结合至抗-FLAG® M2琼脂糖亲和凝胶(A2220)上琼脂糖上的抗-FLAG ®M2单克隆抗体(F1804)进行3X FLAG®融合蛋白的竞争性洗脱。通过亲和色谱法可实现这一目标。用于将3X FLAG融合蛋白从抗-FLAG® M2亲和凝胶上温和的洗脱。1X FLAG肽(F3290)将不会把3X FLAG融合蛋白从抗-FLAG®亲和凝胶上洗脱下来。


浏览我们有关其他应用的参考文献,请访问 FLAG® 文献页面。

制备说明

如需制备储备溶液,可溶解于TBS(含有150 mM NaCl的50mM Tris-HCl,pH7.4)至5 mg/mL的浓度。

法律信息

ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Sigma-Aldrich Co. LLC

储存分类代码

13 - Non Combustible Solids

WGK

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

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SDS

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