Merck
All Photos(4)

F7425

Millipore

抗-FLAG® 兔抗

affinity isolated antibody, buffered aqueous solution

Synonym(s):
抗-ddddk, 抗-dykddddk
MDL编号:
NACRES:
NA.32

生物来源

rabbit

质量水平

200

偶联物

unconjugated

抗体形式

affinity isolated antibody

antibody product type

primary antibodies

克隆

polyclonal

形式

buffered aqueous solution

species reactivity

all

浓度

~0.8 mg/mL

technique(s)

dot blot: 1-2.5 μg/mL
immunoprecipitation (IP): 4-8 μg using amino terminal FLAG-BAP fusion protein from E. coli crude lysate
indirect immunofluorescence: 5-10 μg/mL using 293T cells transfected with a plasmid encoding FLAG-JNK
western blot (chemiluminescent): 1-2.5 μg/mL using an E. coli periplasmic extract expressing an N-terminal FLAG fusion protein

同位素/亚型

IgG

免疫原序列

DYKDDDDK

运输

dry ice

储存温度

−20°C

一般描述

FLAG表位是一种带有肠激酶切割位点的八氨基酸蛋白。
兔抗-FLAG多克隆亲核抗体抗-FLAG可识别位于FLAG融合蛋白上的FLAG表位。该抗体可与N-末端、N末端-Met及C末端FLAG融合蛋白反应。

免疫原

FLAG;肽序列DYKDDDDK

应用

该抗体可识别在N-末端或C-末端对FLAG标记的融合蛋白进行识别,应用于斑点印迹、免疫印迹、免疫沉淀和免疫细胞化学检测。
可通过我们的FLAG®文献端口浏览更多应用参考文献。“

生化/生理作用

FLAG表位在融合蛋白的免疫亲和纯化中具有重要作用。

外形

含有1%牛血清白蛋白和15mM 叠氮化钠的0.01M磷酸盐缓冲盐水(pH7.4)中的溶液

制备说明

在可承受免疫肽的色谱柱上通过亲和色谱进行纯化。

法律信息

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 2

闪点(F)

Not applicable

闪点(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

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SDS

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