Merck

F7879

Sigma-Aldrich

阳离子铁蛋白 来源于马脾脏

10 mg/mL in 0.15 M NaCl

CAS号:
MDL编号:
NACRES:
NA.26

生物来源

horse spleen

质量水平

无菌性

sterile-filtered

形式

liquid

浓度

10 mg/mL in 0.15 M NaCl

technique(s)

cell culture | mammalian: suitable
electron microscopy: suitable

UniProt登记号

储存温度

2-8°C

Gene Information

horse ... FTL(100051593)

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此商品
F6754F6879F4503
form

liquid

form

liquid

form

liquid

form

saline solution

concentration

10 mg/mL in 0.15 M NaCl

concentration

10 μg/mL

concentration

10 μg/mL in 0.15 M NaCl, 10 mM Tris, pH 8.0, containing 0.1% sodium azide

concentration

-

technique(s)

cell culture | mammalian: suitable, electron microscopy: suitable

technique(s)

-

technique(s)

-

technique(s)

-

UniProt accession no.

P02791

UniProt accession no.

P02792, P02794, Q8N4E7

UniProt accession no.

P02792, P02794

UniProt accession no.

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

一般描述

铁蛋白是一种铁贮藏蛋白,通常存在于哺乳动物的肝脏和脾脏中。铁和铁蛋白在眼内的分布基本相同。铁蛋白由重链和轻链组成。

应用

一种阳离子衍生物,用于标记带负电荷的细胞膜,以便电镜观察。
从马脾阳离子化的铁蛋白已用于:
  • 在研究中孵育阿米巴 (106),确定滋养体是否能够吸收铁蛋白并内化该蛋白,使其在无菌培养中生长
  • 通过电子显微镜检查确定吞噬体-溶酶体融合
  • 在无血清孵育介质中标记新鲜切除的鸡视神经盖

铁蛋白是一种铁储存蛋白,在铁代谢中发挥核心角色,在炎症或恶性疾病中可能升高。它在研究中被用作机体铁供应的指标,但在慢性炎症存在时可能被证明是不准确的。

生化/生理作用

铁蛋白将铁保持在其三价铁状态 (Fe 3 + )。铁蛋白的核心具有容纳 4500 个铁分子的能力。在脊椎动物中储存铁是必不可少的。铁蛋白在无脊椎动物中也是储存和运输铁的必需物质,并在膳食铁吸收中起关键作用。

制备说明

将马脾铁蛋白与 N,N-二甲基-1,3-丙二胺 (DMPA) 偶联制备。

储存分类代码

10 - Combustible liquids

WGK

WGK 2

闪点(F)

Not applicable

闪点(C)

Not applicable


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