This product is not charcoal-stripped or hormone-depleted. As a human serum product derived from pooled male plasma from blood group AB, this product may contain naturally occurring levels of estradiol and progesterone. Concentrations of estradiol and progesterone are not listed as tested specifications and are therefore not provided.
H4522
人血清
from human male AB plasma, USA origin, sterile-filtered
别名:
人血浆, 人血清
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| 规格/货号 | 是否有货 | 价格 |
|---|---|---|
20 mL | 请联系客服了解存货情况 | $179.00 |
100 mL | 即日发货发货地SIGMA ALDRICH Pte Ltd | $401.00 |
关于此项目
Biological source:
human male plasma (Type AB)
Origin:
USA origin
Sterility:
sterile-filtered
Shipped in:
dry ice
$179.00
请联系客服了解存货情况
biological source
human male plasma (Type AB)
Quality Segment
sterility
sterile-filtered
composition
hemoglobin, ≤30 mg/dL
origin
USA origin
technique(s)
cell culture | mammalian: suitable, cell culture | stem cell: suitable
impurities
≤10 EU/mL endotoxin
shipped in
dry ice
storage temp.
−20°C
Application
人 AB 血清用于组织工程、移植和细胞治疗应用，可用于扩增来自脂肪组织的间充质干细胞 (MSC) 或来自人骨髓的间充质基质细胞； 用于标准化的角膜缘上皮干细胞移植物生成和移植； 用于在造血干细胞扩增培养基中以外周血体外扩增 NK 细胞； 以及移植前人胰岛培养技术的升级。
人血清已被用于：作为含吐温®去污剂的磷酸盐缓冲液 (PBST)的成分用于桥连酶联免疫吸附试验(ELISA); 作为模拟生物样品用于评估复杂生物样品在纸基ELISA试验中的性能; 作为稀释剂用于制备基线样品的稀释系列，从而为添加NISTmAb的血清和多发性骨髓瘤（MM）患者样品MM2232生成标准曲线
Biochem/physiol Actions
人AB血清通常是通过全血自然凝结而获取，称为Off-The-Clot (OTC) 血清。由凝结全血获取的人血清是首选的血清材料，因为它通常含有较高浓度的更加多样化的生长因子，可实现最优的细胞生长。来自AB 血型供体的人血清没有A型和B型血抗原的特异性抗体。这一特性令其成为通用的供体血清，适用于各种细胞培养，包括人干细胞系。
Packaging
由于产品包装瓶供应不足，不同批次产品的包装可能不同。瓶盖是否包裹热缩膜不会影响产品的完整性/无菌性。
Analysis Note
内毒素检测
Other Notes
我们的人血清产品呈浑浊状并不罕见。该产品来自混合血清，浑浊表明血清中存在脂质。暂无透明、无脂质的人血清产品。
Legal Information
TWEEN is a registered trademark of Croda International PLC
Disclaimer
ELISA 检测每位捐献者的乙型&和丙型肝炎无反应，对人类免疫缺陷病毒 (HIV) 抗体无反应。然而，应将人源产品视为潜在传染性产品并进行相应处理。
仅供研究使用。本品在法国的科研应用受到监管，包括用于进口和出口活动（法国《公共卫生法典》Public Health Code Article L 1211-1 paragraph 2）。如《公共卫生法典》所述，购买者（即最终用户）需要从法国研究部(France Ministry of Research)获得进口授权。订购此产品即代表您已确认获得合法的进口授权。
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此商品 | |||
|---|---|---|---|
| description from human male AB plasma, USA origin, sterile-filtered | description Heat Inactivated, from human male AB plasma, USA origin, sterile-filtered | description from platelet poor human plasma, sterile-filtered, (mycoplasma tested, virus tested) | description Donor herd, USA origin, sterile-filtered, suitable for cell culture, suitable for hybridoma |
| biological source human male plasma (Type AB) | biological source human male plasma (Type AB) | biological source human | biological source horse serum |
| technique(s) cell culture | stem cell: suitable, cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | hybridoma: suitable, cell culture | mammalian: suitable |
| origin USA origin | origin USA origin | origin USA origin | origin USA origin |
| sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered |
| composition hemoglobin, ≤30 mg/dL | composition hemoglobin, ≤20 mg/dL | composition - | composition Hemoglobin, ≤20 mg/dL |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
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存储类别
10 - Combustible liquids
怎么回事？
WGK 3
闪点 (F)
Not applicable
闪点 (°C)
Not applicable
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Does this product contain Estradiol or progesterone or is is already stripped
1 answer-
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Is this product obtained from healthy individuals? I noted that each donor unit used in the preparation was tested and found negative for HBsAG, HIV and etc. However, does each donor was tested for any underlying diseases like Diabetes and medically fit?
1 answer-
This serum product is pooled from multiple donors. Typical pooled batches range from 50 – 70 donors, but large batches may have up to 200. The general health and/or underlying condition information is not collected from individual donors. Based on electrophoresis studies, the pooled final product may be considered ‘Normal’ serum.
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안녕하세요, 혹시 해당 제품의 -20도에서 보관기간을 알 수 있을요?
1 answer-
This product should be stored at -20°C. The storage period at this temperature is valid up to the lot-specific expiration date printed on the product label or COA. The information requested is lot-specific and reported in the Certificate of Analysis. Please see the link below to access a Certificate of lot interest:
https://www.sigmaaldrich.com/product/sigma/h4522#product-documentationHelpful?
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Is this serum contain Uric acid? If yes how much, or how to check it?. If the serum is expired, is it okay to use?
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While uric acid is present in human serum, this product is not tested for uric acid levels. Should uric acid measurement is required, see product MAK483, Uric Acid Assay Kit, which is suitable for the determination of Uric acid in serum. It is not recommended to use products that are beyond the assigned expiration date.
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Once the product has been thawed, how should it be stored? How many times can be freezed and thawed?
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After the initial thaw, the serum can be aliquoted in to single use vials and stored at -20°C. For more information, please refer to the following page:
https://www.sigmaaldrich.com/technical-documents/protocol/cell-culture-and-cell-culture-analysis/cell-culture-media-preparation/storing-and-thawing-serumHelpful?
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Is this Human Serum stock in PBS or any specific buffer?
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This product does not contain phosphate-buffered saline or any specific buffer.
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What is the concentration of Mg2+ and Ca2+ in this serum (H4522)?
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The magnesium levels are not determined for this serum product. The calcium levels are not tested on a lot-to-lot basis. However, historical information indicates calcium concentrations tend to range from 6.5 - 10 mg/dL.
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What is the antibodies concentration in Product H4522, human serum?
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The immunoglobulin concentration of this product is not measured. This is a pooled product from adult AB negative males. Based on information published in the Geigy Scientific Tables, the average immunoglobulin levels for adult males, ages 20 – 70, are as follows:
IgG
10.69 – 12.04 g./L
IgA
1.32 – 2.34 g/L
IgM (not age dependent)
0.77 g/LHelpful?
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Il prodotto è stato testato per negatività a HSV?
1 answer-
Each donor is tested for and found non-reactive to Hepatitis B & C and non-reactive to Human Immunodeficiency Virus (HIV) antibody by ELISA. HSV is not on the list of agents that are tested. Nevertheless, products of human origin should be considered potentially infectious and handled accordingly. See the link below to review a sample or lot specific Certificate of Analysis for this product: https://www.sigmaaldrich.com/product/sigma/i4506#product-documentation
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If this is "serum" derived from "plasma" the plasma would have contained anti-coagulant. To turn the plasma into serum it has to be clotted. Has this product been clotted to remove the fibrin? Or is it still in fact plasma?
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The product has been clotted. Blood is collected and treated with citrate (anticoagulant) which inhibits clotting initially. The blood is then centrifuged. When the spin is complete, layers will appear which allow a differentiation between the cells and the plasma. The plasma is removed and calcium is added to activate the clotting cascade. The preparation is then centrifuged again and the result is a large clot which is pelleted to the bottom of the tube with a serum layer on top.
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