Merck
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H9914

Millipore

HIS-Select® 镍磁性琼脂糖珠

Synonym(s):
nickel charged magnetic beaded agarose
NACRES:
NA.56

偶联物

magnetic beads

质量水平

200

形式

suspension

保质期

2 yr (Unopened product)

基质

6% beaded magnetic agarose

容量

≥15 mg/mL binding capacity

储存温度

2-8°C

一般描述

HIS-选择性磁性琼脂糖珠由顺磁性的固定化金属离子亲和色谱(IMAC)树脂组成,包含专有的四配位体螯合物,与镍结合,通过不带电荷的亲水性连接子共价附于磁性珠状琼脂糖上。磁珠的磁性有助于操作,例如可反复清洗和回收与蛋白结合的磁珠。这使得实验重现性更高,更准确地定量组氨酸标记的目标蛋白。

应用

HIS-选择性镍磁性琼脂糖珠设计用于组氨酸标记蛋白的自动化和小规模亲和捕获(分子pull-down)纯化,同时表现出与其他蛋白非特异性结合低。该珠可在天然和变性条件下纯化His标签蛋白。

外形

以30%乙醇溶液中的50%浆液悬浮液的形式提供。

法律信息

HIS-Select is a registered trademark of Sigma-Aldrich Co. LLC

警示用语:

Danger

危险分类

Aquatic Chronic 3 - Carc. 1A - Flam. Liq. 3 - Repr. 1B - Skin Sens. 1 - STOT RE 2

靶器官

Respiratory Tract

储存分类代码

3 - Flammable liquids

WGK

WGK 3

闪点(F)

86.0 °F

闪点(C)

30 °C

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

SDS

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