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HSRT100

Sigma-Aldrich

增强型禽类HS RT-PCR试剂盒

Flexible kit for one-step or two-step RT-PCR

NACRES:
NA.55

质量水平

200

用途

sufficient for 100 reactions

特点

dNTPs included
hotstart

technique(s)

RT-PCR: suitable

颜色

colorless

input

purified RNA

运输

wet ice

储存温度

−20°C

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应用

增强型禽类HS RT-PCR试剂盒用于合成反转录酶PCR (RT-PCR) 分析的第一条cDNA链。
随附一步法与两步法RT-PCR反应具体步骤。

一步法:在单个反应管中,eAMVTM RT与AccuTaq LA依次参与反应,先生成cDNA,之后立刻通过PCR进行扩增。可快速、灵敏地分析RNA。

两步法:当实验方案需要多步扩增或用户更偏重高得率而非便利性时,可对每一个反应单独优化,以提高得率,同时保持高保真性保真性。

特点和优势

  • 该反转录酶可制备更长的转录本,生成长达14.1kb的cDNA。
  • 低丰度mRNA检测灵敏度更高。eAMV RT能够成功转录其他反转录酶无法检测到的微量RNA。
  • 针对复杂的二级结构的RNA,拥有超强的转录能力。
  • JumpStart AccuTaq LA DNA聚合酶有利于提高灵敏度、特异性与得率。

其他说明

Reverse Transcriptase PCR (RT-PCR)是一种强大的基因表达研究工具。Enhanced Avian HS RT-PCR试剂盒应用了一款加强型禽成髓细胞瘤病毒逆转录酶(eAMV-RT),相比标准型AMV-RT和MMLV-RT具有更佳的反应性能。eAMV RT是一种效率极强的酶,在较高温度下(65 °C)仍能够打开复杂RNA的二级结构成功介导转录反应,是以总RNA或poly(A)+ RNA为模板制备高质量全长cDNA(长达14.1kb)的最佳酶蛋白。同时本品也附赠JumpStartTM AccuTaq LA DNA聚合酶混合物,可减少非特异性扩增,增加反应特异性与灵敏度。这两种酶的组合为用户提供了一个高品质的反应系统,能够同时兼容一步法或两步法的RT-PCR方案。

法律信息

No license is conveyed with the purchase of this product under any of US Patents Nos. 5,804,375, 5,994,056, 6,171,785, 6,214,979, 5,538,848, 5,723,591, 5,876,930, 6,030,787, and 6,258,569, and corresponding patents outside the United States, or any other patents or patent applications, relating to the 5′ Nuclease and dsDNA-Binding Dye Processes. For further information contact the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
AccuTaq is a trademark of Sigma-Aldrich Co. LLC
JumpStart is a trademark of Sigma-Aldrich Co. LLC
eAMV is a trademark of Sigma-Aldrich Co. LLC

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书

  • O4387Random nonamers 100 μL

  • O4387Anchored oligo(dT)23 primers 100 μL

  • B017410x reaction buffers 10x AccuTaq buffer

  • P219210 mM dNTP mix 10 x PCR buffer

  • W1754Nuclease-free water 4 x 1.5

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable

分析证书

原产地证书 (CofO)

Chelsea T Smartt et al.
The American journal of tropical medicine and hygiene, 81(2), 258-263 (2009-07-29)
Alterations in gene expression in the midgut of female Culex pipiens quinquefasciatus exposed to blood meals containing 6.8 logs plaque-forming units/mL of West Nile virus (WNV) were studied by fluorescent differential display. Twenty-six different cDNAs exhibited reproducible differences after feeding
Transgenic chickpea expressing a recombinant human $\alpha$ 1-proteinase inhibitor ($\alpha$ 1-PI) driven by a seed-specific promoters from the common bean Phaseolus vulgaris (L.)
Mishra S, et al.
Plant Cell, Tissue and Organ Culture, 115(1), 23-33 (2013)
Anita Abu-Daya et al.
Developmental biology, 349(2), 204-212 (2010-10-28)
While limb regeneration has been extensively studied in amphibians, little is known about the initial events in limb formation in metamorphosing anurans. The small secreted integrin ligand nephronectin (npnt) is necessary for development of the metanephros in mouse. Although expressed
Nicotiana tabacum osmotic stress-activated kinase is regulated by phosphorylation on Ser-154 and Ser-158 in the kinase activation loop
Burza AM, et al.
The Journal of Biological Chemistry, 281(45), 34299-34311 (2006)
17-estradiol attenuates LPS-induced interleukin-8 production by human peripheral blood monocytes through estrogen receptor-activation
Malisorn N, et al.
African Journal of Pharmacy and Pharmacology, 4(11), 806-810 (2010)

实验方案

The 3'/5' Assay for Analysis of RNA Integrity Protocol

The 3’/5’ integrity assay is a potential first step in the identification of RNA degradation. The assay is particularly useful when a large number of samples are to be analyzed or when the degradation is less than that detected by capillary systems but still sufficient to effect qPCR analyses.

Long and Accurate PCR Amplification of DNA with RedAccuTaq® (D4812)

Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures. Red dye allows direct loading of reaction on a gel. REDAccuTaq LA

相关内容

逆转录

分析基因表达的方法之一是测量基因的mRNA浓度。此类分析面临着若干挑战,例如不同转录本之间的半衰期不同、转录时间模式以及mRNA和蛋白质之间缺乏相关性。

Reverse Transcription

One approach to the analysis of gene expression is to measure the concentration of mRNA of a gene. There are several challenges to such analyses, such as the differences in half life between different transcripts, the temporal patterns of transcription and the lack of correlation between mRNA and protein.

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