Merck

L2524

Sigma-Aldrich

溶壁酶 来源于藤黄节杆菌

lyophilized powder, ≥2,000 units/mg protein, Protein ≥20 % by biuret

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别名:
(1,3)-β-D-Glucan endohydrolase, 1,3-β-Glucan glucohydrolase, Bacterial lyticase, Lysing enzyme
CAS号:
MDL编号:
NACRES:
NA.54

生物来源

bacterial (Arthrobacter luteus)

质量水平

形式

lyophilized powder

比活

≥2,000 units/mg protein

组成

Protein, ≥20% biuret

技术

cell based assay: suitable

适用性

suitable for cell lysis

应用

diagnostic assay manufacturing

储存温度

−20°C

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此商品
L4025A6814A6380
溶壁酶 来源于藤黄节杆菌 lyophilized powder, ≥2,000 units/mg protein, Protein ≥20 % by biuret

L2524

溶壁酶 来源于藤黄节杆菌

溶壁酶 来源于藤黄节杆菌 lyophilized powder, ≥200 units/mg solid

L4025

溶壁酶 来源于藤黄节杆菌

α-淀粉酶 来源于芽孢杆菌 属 powder, ≥400 units/mg protein (Lowry)

A6814

α-淀粉酶 来源于芽孢杆菌

α-淀粉酶 来源于芽孢杆菌 属 Type II-A, lyophilized powder, ≥1,500 units/mg protein (biuret)

A6380

α-淀粉酶 来源于芽孢杆菌

specific activity

≥2,000 units/mg protein

specific activity

≥200 units/mg solid

specific activity

≥400 units/mg protein (Lowry)

specific activity

≥1,500 units/mg protein (biuret)

biological source

bacterial (Arthrobacter luteus)

biological source

bacterial (Arthrobacter luteus)

biological source

Bacillus sp.

biological source

bacterial (Bacillus amyloliquefaciens)

suitability

suitable for cell lysis

suitability

suitable for cell lysis

suitability

-

suitability

suitable for hydrolysis, synthesis of oligosaccharides and polysaccharides, and sugar modification

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

-

application(s)

life science and biopharma

form

lyophilized powder

form

lyophilized powder

form

powder

form

lyophilized powder

应用

藤黄节杆菌(Arthrobacter luteus )裂解酶已用于裂解真菌细胞壁,分离DNA。
Lyticase from Arthrobacter luteus has been used:
  • for spheroplasting the cells
  • as a component of digestion solution to incubate yeast cells for digestion of the cell wall
  • in the enzymatic hydrolysis of the mycelium precipitate to prepare protoplasts

生化/生理作用

酵母细胞很难被破坏,因为其细胞壁可能形成胶囊或耐药孢子。可通过裂解酶(如溶壁酶)诱导部分原生质球形成,从酵母中提取DNA。随后再裂解原生质球以释放DNA。溶壁酶更易消化酵母菌细胞壁,并从真菌中产生原生质球进行转化。它含有β-(1→3)-葡聚糖昆布五糖水解酶以及β-(1→3)-葡聚糖酶、蛋白酶和甘露聚糖酶活性。溶壁酶适用于多种酵母细胞,例如念珠菌(Candida)、德巴利酵母菌( Debaryomyces)、酿酒酵母(Saccharomyces)、覆膜酵母(Saccharomycopsis)、类酵母酵(Saccharomycodes)、出鞘菌属(Eremothecium)和许旺酵母(Schwanniomyces species)。
Lyticase enzyme is frequently used in fungal research, particularly for species identification using polymerase chain reaction (PCR)-based techniques. It can break down β (1→3) and β (1→4) bonds between glucose units. Lysozyme serves as an indicator of macrophage-mediated host response, correlates with white cell death, and exhibits a high turnover rate. Elevated levels of serum lysozyme have been observed in various chronic inflammatory conditions, inflammatory bowel diseases, hematological disorders, and renal disorders. The c-type lysozyme from hen egg white is commonly used as a model for studying protein structure and function. Muramidase primarily exhibits bactericidal activity against Gram-positive bacteria. The method described in the bulletin for determining molecular masses using gel filtration chromatography is a modified version of existing published techniques. The protein standards included in this kit may be compatible with other chromatographic systems like high-performance liquid chromatography (HPLC). However, certain buffer systems may affect the elution volumes of albumin and carbonic anhydrase. The proteins in this kit have a molecular mass range spanning from 29 kDa to 699 kDa.
溶细胞酶水解聚-β(1→3)-葡萄糖,如酵母细胞壁葡聚糖。

单位定义

一个单位在25°C和pH 7.5条件下,在3ml反应混合物中以酵母悬浮液为底物,每分钟产生0.001的ΔA800

外形

部分纯化的冻干粉末,含有磷酸钾缓冲盐和稳定剂

其他说明

For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.

象形图

Health hazard

警示用语:

Danger

危险声明

预防措施声明

危险分类

Resp. Sens. 1

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


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实验方案

This procedure may be used for the determination of Lyticase activity using Baker’s yeast as the substrate.

本操作程序可用于使用面包酵母作为底物测定溶壁酶的活性。

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