Merck
All Photos(2)

L2524

Sigma-Aldrich

溶壁酶 来源于藤黄节杆菌

lyophilized powder, ≥2,000 units/mg protein, Protein ≥20 % by biuret

Synonym(s):
(1,3)-β-D-Glucan endohydrolase, 1,3-β-Glucan glucohydrolase
CAS Number:
MDL编号:
NACRES:
NA.54

形式

lyophilized powder

质量水平

300

specific activity

≥2,000 units/mg protein

组成

Protein, ≥20% biuret

application(s)

diagnostic assay manufacturing

储存温度

−20°C

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应用

Lyticase from Arthrobacter luteus has been used to lyse the fungal cell wall for DNA isolation.

包装

10000, 25000 units in glass bottle
50000, 200000 units in poly bottle

生化/生理作用

Yeast cells are difficult to disrupt because the cell walls may form capsules or resistant spores. DNA can be extracted from yeast by using lysing enzymes such as lyticase to induce partial spheroplast formation. Spheroplasts are subsequently lysed to release DNA. Lyticase is preferred to digest the cell walls of yeast and generate spheroplasts from fungi for transformation. It contains β-(1→3)-glucan laminaripentaohydrolase along with β-(1→3)-glucanase, protease, and mannanase activities. Lyticase is used for yeast cells like Candida, Debaryomyces, Saccharomyces, Saccharomycopsis, Saccharomycodes, Eremothecium, and Schwanniomyces species.
溶细胞酶水解聚-β(1→3)-葡萄糖,如酵母细胞壁葡聚糖。

单位定义

一个单位在25°C和pH 7.5条件下,在3ml反应混合物中以酵母悬浮液为底物,每分钟产生0.001的ΔA800

外形

部分纯化的冻干粉末,含有磷酸钾缓冲盐和稳定剂

象形图

Health hazard

警示用语:

Danger

危险声明

危险分类

Resp. Sens. 1

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

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