Merck

M3807

Sigma-Aldrich

Anti-MAP Kinase, Non-Phosphorylated ERK antibody, Mouse monoclonal

clone ERK-NP2, purified from hybridoma cell culture

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别名:
Anti-ERK, Anti-ERK-2, Anti-ERK2, Anti-ERT1, Anti-MAPK2, Anti-NS13, Anti-P42MAPK, Anti-PRKM1, Anti-PRKM2, Anti-p38, Anti-p40, Anti-p41, Anti-p41mapk, Anti-p42-MAPK
MDL编号:
NACRES:
NA.44

生物来源

mouse

质量水平

偶联物

unconjugated

抗体形式

purified from hybridoma cell culture

抗体产品类型

primary antibodies

克隆

ERK-NP2, monoclonal

形式

buffered aqueous solution

分子量

antigen, ERK-1 44 kDa
antigen, ERK-2 42 kDa

种属反应性

human, rat

浓度

~2 mg/mL

技术

immunocytochemistry: suitable
indirect ELISA: suitable
microarray: suitable
western blot: 5-25 μg/mL using rat brain extract

同位素/亚型

IgG1

UniProt登记号

运输

dry ice

储存温度

−20°C

靶向翻译后修饰

unmodified

基因信息

一般描述

Mitogen-activated protein kinase (MAPK) superfamily of enzymes is involved in widespread signalling pathways. Members of this family include the ERK1/2 (extracellular signal-regulated protein kinase, also termed p42/p44 MAPK), JNK and p38 MAPK subfamilies. These are the terminal enzymes in a signalling cascade where each kinase phosphorylates and activates the next member in the sequence. Phosphorylation of both tyrosine and threonine is essential for the full activation of all MAPKs. Several kinases participate in activation of the ERK cascade. This cascade is initiated by the small G protein Ras, which upon stimulation causes activation Raf1 kinase. Raf1 continues the transmission by activating MEK. Activated MEK appears to be the only kinase capable of specifically phosphorylating and activating ERK. ERK appears to be an important regulatory molecule, which by can phosphorylate regulatory targets in the cytosol (phospholipase A2, PLA2), translocated into and phosphorylate substrates in the nucleus (ELK1). The activation of ERK cascade mediates and regulates the signal transduction pathways in response to stress, mitogenic signals and is important in development and differentiation, learning, memory and survival.

特异性

The antibody reacts specifically with the non-phosphorylated, non-activated form of MAP kinase (ERK-1 and ERK-2). Weak cross-reaction is observed with monophosphorylated (threonine or tyrosine), but not with diphosphorylated peptides of MAPK. The antibody does not recognize JNK or p38 MAPK. The epitope recognized by the antibody contains non-phosphorylated threonine 183 and tyrosine 185 which resides within the ERK-activation loop (e.g., amino acids 178-188 in ERK-2).

免疫原

synthetic peptide HTGFLTEYVAT, corresponding to the non-phosphorylated form of ERK-activation loop.

应用

A working concentration of 5-25 μg/mL may be used for detection by immunoblotting in rat brain extract. The antibody has also been used at working dilution of 1:50 for MAPK quantification by flow cytometry in monocyte-derived macrophages (MDM) derived from Holstein cattle.

外形

Solution in phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

制备说明

Prepared from a culture supernatant of bioreactor grown hybridoma

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存分类代码

10 - Combustible liquids

WGK

nwg

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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