Merck

M5909

Sigma-Aldrich

IgM Isotype Control from murine myeloma

clone MOPC 104E, 200 μg/mL, buffered aqueous solution, purified immunoglobulin

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别名:
Mouse IgM Isotype Control from myeloma
MDL编号:
NACRES:
NA.46

生物来源

mouse

质量水平

偶联物

unconjugated

抗体形式

purified immunoglobulin

克隆

MOPC 104E, monoclonal

形式

buffered aqueous solution

浓度

200 μg/mL

技术

flow cytometry: suitable

同位素/亚型

IgMλ

运输

wet ice

储存温度

2-8°C

靶向翻译后修饰

unmodified

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此商品
M5409M5284F6397
IgM Isotype Control from murine myeloma clone MOPC 104E, 200 μg/mL, buffered aqueous solution, purified immunoglobulin

M5909

IgM Isotype Control from murine myeloma

clone

MOPC 104E, monoclonal

clone

UPC-10, monoclonal

clone

MOPC 21, monoclonal

clone

MOPC 21, monoclonal

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

isotype

IgMλ

isotype

-

isotype

-

isotype

-

一般描述

IgM is a glycoprotein antibody that regulates humoral immune responses. Mouse IgM is involved in modulating B cell memory . This antibody isotype has also been implicated in the development of autoimmune responses associated with the pathogenesis of type 1 diabetes in mice . IgM isotype control antibody is specific for anti-mouse whole serum and anti-mouse IgM. The product does not react with antisera to mouse IgA, IgG1, IgG2a, IgG2b, and IgG3.
Isotype controls are non-reactive immunoglobulins of the same isotype as the primary antibody being used in an application. It is recommended that a non-reactive immunoglobulin of the same isotype and concentration be included as a negative control for each monoclonal antibody reagent used in flow cytometry or other immunoassays.
The purified IgM Isotype Control clone MOPC 104E preparation is non-reactive with antisera to mouse IgA, IgG1, IgG2a, IgG2b, and IgG3. When evaluated in flow cytometry, the IgM Isotype Control did not stain human peripheral blood lymphocytes (PBLs). A FITC Goat anti-Mouse IgM (μ-chain specific), Affinity Isolated Antibody (Product No. F 9259) along with 1 μg of the product was incubated with human PBLs and then evaluated by flow cytometry.

应用

IgM Isotype Control clone MOPC 104E is recommended as a negative control in flow cytometry. Working Concentration: equal concentrations of isotype control and primary antibody are recommended for use in flow cytometry. Equal concentrations of isotype control and primary antibody are recommended for use in flow cytometry.
IgM Isotype Control from murine myeloma has been used:
  • in chromatin immunoprecipitation (ChIP) assay
  • LABScreen assays
  • immunofluorescent staining

IgM isotype control antibodies are suitable for use in immunohistochemistry .

原理

The specificity of staining by monoclonal antibodies to mouse CD antigens should be verified by establishing the non-specific reagent binding to the target cell population. It is recommended that a concentration and isotype matched mouse myeloma immunoglobulin be included as a negative control for each monoclonal antibody reagent used in the immunoassay procedure.
Sigma Mouse Isotype Controls are supplied to match the concentration of Sigma purified and/or conjugated monoclonal antibodies to CD antigens. DUAL-TAG controls contain 100 μg of each conjugate.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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