Merck

M8177

Sigma-Aldrich

Anti-p38 MAP Kinase, Activated (Diphosphorylated p38) antibody, Mouse monoclonal

clone P38-TY, purified from hybridoma cell culture, buffered aqueous solution

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别名:
Anti-MAP Kinase, p38, Activated, Monoclonal Anti-p38 MAP Kinase, Activated (Diphosphorylated p38) antibody produced in mouse
MDL编号:

生物来源

mouse

质量水平

偶联物

unconjugated

抗体形式

purified from hybridoma cell culture

抗体产品类型

primary antibodies

克隆

P38-TY, monoclonal

形式

buffered aqueous solution

种属反应性

rat, human, mouse

浓度

~2 mg/mL

技术

immunocytochemistry: suitable
indirect ELISA: suitable
microarray: suitable
western blot: 1 μg/mL using whole cell extract from a rat fibroblast cell line, Rat1, activated with sorbitol

同位素/亚型

IgG2a

UniProt登记号

运输

dry ice

储存温度

−20°C

靶向翻译后修饰

unmodified

基因信息

human ... MAPK14(1432)
mouse ... Mapk14(26416)
rat ... Mapk14(81649)

相关类别

一般描述

Monoclonal Anti-p38 MAP Kinase, Activated (Diphosphorylated p38) (mouse IgG2a isotype) is derived from the P38-TY hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. The p38 mitogen-activated protein kinases (MAPK) comprises the stress-activated protein kinase (SAPK) 2b, SAPK3 and SAPK4 (also termed p38β-δ). All these kinases contain a glycine residue in their TXY(Thr−Xaa−Tyr motif) activation motif.

特异性

Reacts specifically with the active diphosphorylated form of p38 MAP kinase and its closely related isoforms. Does not recognize the non-phosphorylated and mono­phosphory­lated forms of the p38 MAP kinase molecule, or the non-phosphorylated, monophosphorylated and diphosphorylated forms of JNK and ERK-MAP kinases. The epitope recognized by the antibody contains the phosphorylated threonine and tyrosine residues within the regulatory site of active p38 MAP kinase (Thr180-Gly181-Tyr182).

免疫原

Synthetic peptide HTDDEMpTGpYVATR corresponding to the phosphorylated form of p38 MAP kinase activation loop, coupled to KLH.

应用

Monoclonal Anti-p38 MAP Kinase, Activated (Diphosphorylated p38) antibody produced in mouse may be used in immunoblotting, enzyme linked immunosorbent assay (ELISA), immunostaining, western blotting, immunohistochemistry and in immunocytochemistry.

生化/生理作用

The p38 mitogen-activated protein kinases (MAPK) are activated by phosphorylation of both tyrosine (Y) and threonine (T) residues organized in a TXY motif. The residue in between the two phosphorylated residues determines the specificity of activation of the MAPKs, and is glutamic acid for extracellular-signal regulated kinase (ERK) (TEY), proline for c-Jun N-terminal kinases (JNK) and glycine for p38 MAPK. Phosphorylation of both tyrosine and threonine is essential for the full activation of all MAPKs. ERK generally transmits signals leading to cell proliferation, p38 MAPK and JNK are both mostly stress-responsive kinases3 and have been implicated in cell death in several cellular systems. Antibodies reacting specifically with activated p38 MAPK are useful tools in the study of the intracellular location of p38 MAPK enzymes, and in sorting out the signal transduction pathways of the MAPK superfamily.

外形

0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。

制备说明

From a culture supernatant of bioreactor grown hybridoma.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存分类代码

10 - Combustible liquids

WGK

nwg

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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Gian F De Nicola et al.
JCI insight, 3(16) (2018-08-24)
Inhibiting MAPK14 (p38α) diminishes cardiac damage in myocardial ischemia. During myocardial ischemia, p38α interacts with TAB1, a scaffold protein, which promotes p38α autoactivation; active p38α (pp38α) then transphosphorylates TAB1. Previously, we solved the X-ray structure of the p38α-TAB1 (residues 384-412)
The p38 MAPK stress pathway as a tumor suppressor or more
Mathew L andGuan C
Frontiers in Bioscience, 13, 3581-3581 (2008)
Matrix metalloproteinase-1 is regulated in tuberculosis by a p38 MAPK-dependent, p-aminosalicylic acid-sensitive signaling cascade
Rand L, et al.
Journal of Immunology, 182(9), 5865-5872 (2009)
Effect of IL-11 on glomerular expression of TGF-beta and extracellular matrix in nephrotoxic nephritis in Wistar Kyoto rats
Stangou M, et al.
Journal of Nephrology, 24(1), 106-106 (2011)
Simultaneous measurement of ERK, p38, and JNK MAP kinase cascades in vascular smooth muscle cells
Chevalier D, et al.
Journal of Pharmacological and Toxicological Methods, 44(2), 429-439 (2000)

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