Merck
All Photos(1)

M8787

Sigma-Aldrich

氯化镁 溶液

PCR Reagent, 25 mM MgCI2 solution for PCR

Synonym(s):
PCR optimization
Linear Formula:
MgCl2
CAS Number:
Molecular Weight:
95.21
MDL编号:
PubChem化学物质编号:
NACRES:
NA.52

等级

PCR Reagent

质量水平

300

形式

liquid

包装

vial of 1.5 mL

浓度

25 mM±1 mM

technique(s)

PCR: suitable

颜色

colorless

application(s)

agriculture
diagnostic assay manufacturing

异质活性

DNase, RNase, none detected

储存温度

2-8°C

SMILES string

Cl[Mg]Cl

InChI

1S/2ClH.Mg/h2*1H;/q;;+2/p-2

InChI key

TWRXJAOTZQYOKJ-UHFFFAOYSA-L

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一般描述

氯化镁溶液适用于聚合酶链式反应(PCR)优化。氯化镁溶液提供聚合酶链式反应(PCR)所需镁离子。影响引物-模板退火温度、保真度、特异性和产量。过量镁会导致非特异性产物增加,镁缺乏会导致产量降低。

应用

氯化镁溶液作为组分可用于配制:
  • 聚合酶链式反应(PCR)的扩增混合液
  • 简单重复序列区间(ISSR)-PCR的扩增混合液
  • 16S-23S rRNA基因内转录间隔区(ITS)PCR扩增和限制性分析(ITS-PCR-RFLP)的反应缓冲液

适用性

适用于聚合酶链反应的优化。

储存分类代码

12 - Non Combustible Liquids

WGK

nwg

闪点(F)

Not applicable

闪点(C)

Not applicable

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificate of Analysis

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Certificate of Origin

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