Merck

MAK057

Sigma-Aldrich

柠檬酸检测试剂盒

sufficient for 100 colorimetric or fluorometric tests

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NACRES:
NA.84

用途

sufficient for 100 colorimetric or fluorometric tests

application(s)

cosmetics
food and beverages

detection method

colorimetric
fluorometric

相关疾病

cancer

储存温度

−20°C

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Citrate Assay Kit sufficient for 100 colorimetric or fluorometric tests

Sigma-Aldrich

MAK057

柠檬酸检测试剂盒

Aconitase Assay Kit sufficient for 100 colorimetric tests

Sigma-Aldrich

MAK337

Aconitase Assay Kit

detection method

colorimetric, fluorometric

detection method

colorimetric, fluorometric

detection method

colorimetric

detection method

colorimetric

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

application(s)

cosmetics
food and beverages

application(s)

-

application(s)

-

application(s)

-

relevant disease(s)

cancer

relevant disease(s)

cancer

relevant disease(s)

cancer

relevant disease(s)

endocrinological disorders, diabetes; neurological disorders; rheumatological diseases

一般描述

柠檬酸是三羧酸(TCA)循环的关键中间体,通过向乙酰辅酶A的乙酰基中加入草酰乙酸而得。柠檬酸通过柠檬酸-苹果酸梭子从线粒体中转运出来,然后转化回乙酰辅酶A进行脂肪酸合成。柠檬酸是脂肪酸合成和糖酵解的别构调节剂,分别通过作用于乙酰辅酶A羧化酶和磷酸果糖激酶来实现。由于性别、年龄和各种其他因素(包括疾病状态),柠檬酸的代谢和分布差异可能很大。前列腺癌细胞中的细胞柠檬酸水平降低,柠檬酸水平可能是前列腺癌生长速率的标志物。柠檬酸检测试剂盒提供了一种可简单、灵敏、快速地对各种样品中的柠檬酸进行定量的方法。

应用

柠檬酸检测试剂盒用于检测样品中柠檬酸的浓度。

适用性

适用于对包括组织和细胞在内的多种样品中的柠檬酸盐进行测定

原理

柠檬酸的浓度通过酶偶联法检测,产生与存在的柠檬酸成比例的比色(570 nm)/荧光(λex = 535/λem = 587 nm)产物。柠檬酸检测试剂盒的灵敏度在各种样品中通常为0.1至10 nM(2 μmM-10 mM)柠檬酸。

相关产品

产品编号
说明
价格

危险声明

预防措施声明

危险分类

Aquatic Chronic 3

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(°F)

188.6 °F - closed cup

闪点(°C)

87 °C - closed cup


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Long Ding et al.
The EMBO journal, 37(17) (2018-07-28)
Seipin, the gene that causes Berardinelli-Seip congenital lipodystrophy type 2 (BSCL2), is important for adipocyte differentiation and lipid homeostasis. Previous studies in Drosophila revealed that Seipin promotes ER calcium homeostasis through the Ca2+-ATPase SERCA, but little is known about the
High-intensity sprint training inhibits mitochondrial respiration through aconitase inactivation.
Larsen F J, et al.
Faseb Journal, 30(1), 417-427 (2015)
Yan Huang et al.
Regenerative biomaterials, 7(2), 221-232 (2020-04-17)
The purpose of this article was to explore the effects of gold nanoparticles (GNPs) and silver nanoparticles (SNPs) with different cytotoxicities on human dermal fibroblasts (HDFs) at the metabolic level. First, ∼20 nm of GNPs and SNPs were prepared, and their
Jade D Bailey et al.
Cell reports, 28(1), 218-230 (2019-07-04)
Classical activation of macrophages (M(LPS+IFNγ)) elicits the expression of inducible nitric oxide synthase (iNOS), generating large amounts of NO and inhibiting mitochondrial respiration. Upregulation of glycolysis and a disrupted tricarboxylic acid (TCA) cycle underpin this switch to a pro-inflammatory phenotype.
Cheng Zhang et al.
Cell reports, 21(8), 2058-2065 (2017-11-23)
We discovered that induced pluripotent stem cell (iPSC) clones generated from aged tissue donors (A-iPSCs) fail to suppress oxidative phosphorylation. Compared to embryonic stem cells (ESCs) and iPSCs generated from young donors (Y-iPSCs), A-iPSCs show poor expression of the pluripotent

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