推荐产品
用途
sufficient for 100 colorimetric tests
品質等級
檢測方法
colorimetric
儲存溫度
−20°C
基因資訊
human ... PDHA1(5160) , PDHA2(5161 ) , PDHB(5162) , PDHX(8050)
mouse ... PDHA1(18597) , PDHA2(18598) , PDHB(68263) , PDHX(27402)
rat ... PDHA1(29554) , PDHA2(117098) , PDHB(289950) , PDHX(311254)
一般說明
Pyruvate Dehydrogenase (PDH) is a mitochondrial enzyme which has an important role in carbohydrate metabolism. Combining with dihydrolipoyl transacetylase (E2) and dihydrolipoyl dehydrogenase (E3), PDH (E1) forms a well-characterized enzyme complex (PDC). In the presence of NAD+ and CoA, PDH catalyzes the conversion of pyruvate into Acetyl-CoA and CO2. Thus, PDH links the glycolysis metabolic pathway (cytoplasm) to the citric acid cycle (mitochondria). The Pyruvate Dehydrogenase Activity Assay Kit provides a sensitive, simple, fast, direct and convenient way for the detection of PDH activity in various samples, based on a colorimetric method. In the assay, PDH activity changes a colorless developer to a colored product. The amount of the colorimetric (450 nm) product is proportional to the enzymatic activity present in the sample. The kit can detect PDH activity that is lower than 0.1 mU, in a variety of samples.
應用
Pyruvate Dehydrogenase (PDH) Activity Assay Kit has been used in the colorimetric measurement of PDH Activity in biological samples.
特點和優勢
Sensitive, simple, fast, direct and convenient way for the detection of PDH activity in various biological samples.
Supportive calculator will calculate the reagents needed according to your experimental needs, and analyze your results based on your experimental data.
Click here to download a calculator excel file
Supportive calculator will calculate the reagents needed according to your experimental needs, and analyze your results based on your experimental data.
Click here to download a calculator excel file
適合性
Suitable for measuring PDH Activity in adherent or suspension cells, from an animal or plant source.
單位定義
One unit of pyruvate dehydrogenase is the amount of enzyme that will generate 1.0 µmole of NADH per minute at 37 °C at pH=7.5.
訊號詞
Danger
危險分類
Carc. 2 - Eye Irrit. 2 - Muta. 2 - Resp. Sens. 1 - Skin Irrit. 2
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 3
Structural basis for inactivation of the human pyruvate dehydrogenase complex by phosphorylation: role of disordered phosphorylation loops
Kato M, et al.
Structure (Oxford, United Kingdom), 16(12), 1849-1859 (2008)
An improved spectrophotometric assay of pyruvate dehydrogenase in lactate dehydrogenase contaminated mitochondrial preparations from human skeletal muscle
Chretien D, et al.
Clinica Chimica Acta; International Journal of Clinical Chemistry, 240(2), 129-136 (1995)
A key role for mitochondrial gatekeeper pyruvate dehydrogenase in oncogene-induced senescence
Kaplon J, et al.
Nature, 498(7452), 109-112 (2013)
Rapid intramolecular coupling of active sites in the pyruvate dehydrogenase complex of Escherichia coli: mechanism for rate enhancement in a multimeric structure
Danson M J, et al.
Proceedings of the National Academy of Sciences of the USA, 75(11), 5386-5390 (1978)
Self-assembly and catalytic activity of the pyruvate dehydrogenase multienzyme complex from Bacillus stearothermophilus
Domingo G J, et al.
European Journal of Biochemistry, 266(3), 1136-1146 (1999)
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门