Merck

P0056

Sigma-Aldrich

Anti-POU5F1 (Oct4) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

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别名:
Anti-OCT3, Anti-OCT4, Anti-OTF3, Anti-OTF4, Anti-Octamer-binding transcription factor 3, Anti-Pou domain, Class 5, Transcription factor 1

生物来源

rabbit

质量水平

偶联物

unconjugated

抗体形式

affinity isolated antibody

antibody product type

primary antibodies

克隆

polyclonal

形式

buffered aqueous solution

分子量

antigen ~43 kDa

species reactivity

human

浓度

~1.0 mg/mL

technique(s)

immunoprecipitation (IP): 5-10 μg using NT2 cell lysate
indirect immunofluorescence: 2-5 μg/mL using paraformaldhyde-fixed F9 cells
western blot: 1-2 μg/mL using NT2 cell lysate

UniProt登记号

运输

dry ice

储存温度

−20°C

target post-translational modification

unmodified

Gene Information

human ... POU5F1(5460)
mouse ... Pou5f1(18999)
rat ... Pou5f1(294562)

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O8389P0082SAB1404228
conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

clone

polyclonal

clone

polyclonal

clone

PF-29, monoclonal

clone

4F8, monoclonal

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

mol wt

antigen ~43 kDa

mol wt

-

mol wt

antigen ~43 kDa

mol wt

antigen ~35.24 kDa

一般描述

POU class 5 homeobox 1 (POU5F1) recognizes defined octamer DNA element by a shared, conserved DNA-binding domain, namely the POU domain.
POU class 5 homeobox 1 (POU5F1), also known as octamer-binding transcription factor-4 (OCT-4), is expressed in embryonic stem cells and germ cells. It belongs to the POU family of transcription factors. Three splice variants of the protein (OCT4A, OCT4B and OCT4B1) have been identified. The gene encoding this transcription factor is localized on human chromosome 6p21.33.

免疫原

synthetic peptide corresponding to amino acids 19-34 of human POU5F1, conjugated to KLH. The corresponding sequence in rat is 81% homologous.

应用

Anti-POU5F1 (Oct4) antibody produced in rabbit has been used in Western Blotting.

生化/生理作用

POU class 5 homeobox 1 (POU5F1) may be a tumor initiating stem-like cell (TSC)-related marker. It has a role in stem cell pluripotency and self-renewal, and also modulates differentiation. The protein has been shown to be expressed in several cancers. POU5F1 is also vital for the induced pluripotent stem cell (iPSC) process.
POU5F1 or Oct4/Oct3 (Octamer-binding transcription factor), functions as an early developmental control gene. It has been recognized as fundamental in the maintenance of pluripotency in embryonic stem cells and in primordial germ cells. It is down regulated in all differentiated somatic cell types both in vivo and in vitro. This is being accomplished through increased DNA methylation and structural changes involving the immediate upstream regulatory region. POU5F1 serves as a marker for germ cell tumors (GCTs) eliciting pluripotentiality, especially including embryonal, seminoma/ dysgerminoma/ germinoma.

外形

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存分类代码

10 - Combustible liquids

闪点(F)

Not applicable

闪点(C)

Not applicable

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Characterization of POU5F1 (OCT4) gene and its promoter in buffalo ESC-like cells identifies multiple transcription start sites and expression of four pseudogenes
Natwar Singh
A Comprehensive Guide to the Hazardous Properties of Chemical Substances (2012)
Hepatitis C virus core protein regulates OCT4 expression and promotes cell cycle progression in hepatocellular carcinoma
Jia-Jia Zhou
Oncology Reports (2016)
Differentiation of human embryonic stem cells induces condensation of chromosome territories and formation of heterochromatin protein 1 foci
Eva B
Phosphorus-Nitrogen Compounds: Cyclic, Linear, and High Polymeric Systems (2008)
STAT3 inhibitor enhances chemotherapy drug efficacy by
modulating mucin 1 expression in non-small cell lung
carcinoma
Yunguo Han
Tropical Journal of Pharmaceutical Research (2017)
Huiping Tan et al.
Medical science monitor : international medical journal of experimental and clinical research, 24, 3644-3652 (2018-06-01)
BACKGROUND Recent studies have shown that increased mobilization of Long Interspersed Nuclear Elements-1 (L1) can promote the pathophysiology of multiple neurological diseases. However, its role in Huntington's disease (HD) remains unknown. MATERIAL AND METHODS R6/2 mice - a common mouse

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