Merck
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P1860

Sigma-Aldrich

Protease Inhibitor Cocktail

for use in tissue culture media, DMSO solution

Synonym(s):
protease inhibitor
EC 号:
MDL编号:
NACRES:
NA.77

质量水平

形式

DMSO solution

运输

dry ice

储存温度

−20°C

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一般描述

The Protease Inhibitor Cocktail is a mixture of protease inhibitors designed to prevent proteolytic degradation of secreted proteins in tissue culture media.

特异性

抑制丝氨酸、半胱氨酸、和天冬氨酸蛋白酶和氨肽酶

应用

This product is specifically optimized for use in tissue culture media and can be added after 48 hours of exposure to fresh medium to ensure the continued inhibition of proteases.

包装

1 mL in glass bottle

特点和优势

Broad specificity: Inhibits serine, cysteine, aspartic proteases, and aminopeptidases

Non-toxic: After 48 hours exposure, the product is non-toxic to adherent cell lines A431, CHO, COS, HepG2 and HeLa; and to Jurkat and HL-60 cell lines grown in suspension.

Contains no metal chelators: Ensures compatibility with downstream applications

Convenient packaging: 1 mL in glass bottle

Easy to use: Use at a dilution of 1:200 or more in tissue culture media to prevent proteolytic degradation of secreted proteins.

组分

抑肽酶
抑氨肽酶
E-64
亮肽素
胃蛋白酶抑制剂A

其他说明

蛋白酶抑制剂的混合物,具有广泛的特异性,可抑制丝氨酸、半胱氨酸、天冬氨酸和氨肽酶。其含有抑肽酶、抑氨肽酶、亮肽素、E-64、以及胃蛋白酶抑制剂A。其不含金属螯合剂

注意

孵育48小时后,产物对贴壁细胞系A431、CHO、COS、HepG2和HeLa,以及悬浮培养的Jurkat和HL-60细胞系无毒。

数量

在组织培养基中以1:200或更高的稀释度使用,以防止分泌蛋白的蛋白水解降解。

外形

溶于DMSO中(D 2650,Hybri-Max)。

储存分类代码

10 - Combustible liquids

WGK

WGK 1

闪点(F)

188.6 °F

闪点(C)

87 °C

Certificate of Analysis

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Certificate of Origin

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