P8849

Protease Inhibitor Cocktail

for use in purification of Histidine-tagged proteins, DMSO solution

• MDL编号: MFCD00677817
• NACRES: NA.77

Properties

• 生物来源: synthetic
• 质量水平: 300
• 形式: DMSO solution
• 储存温度: −20°C

Description

一般描述

DMSO solution designed for use in the purification of histidine-tagged proteins.

The cocktail contains a mixture of inhibitors that specifically target serine, cysteine, aspartic, and thermolysin-like proteases, and aminopeptidases.

特异性

抑制丝氨酸，半胱氨酸，天冬氨酸，和嗜热菌蛋白酶样蛋白酶和氨基肽酶。

应用

The cocktail has been optimized and tested for isolation of histidine-tagged proteins, with chelating agents omitted for compatibility with IMAC applications.

It is recommended for inhibition of protease activity in 100 mL of cell lysate from 20 g of Escherichia coli or 10 g of baculovirus-infected Spodoptera frugiperda pupal ovary cells.

包装

1 mL in poly bottle

5 mL in glass bottle

特点和优势

Specifically formulated for histidine-tagged protein purification.

Targets multiple types of proteases, ensuring comprehensive inhibition of protease activity.

Compatibility with IMAC applications due to omission of chelating agents.

Supplied in convenient packaging options.

组分

AEBSF
乌苯美司
E-64
Pepstatin A
磷阿米酮

数量

推荐采用1mL抑制从20gEscherichia coli或10g杆状病毒感染 Spodoptera frugiperda蛹卵巢细胞提取的总体积100ml的蛋白酶。

该蛋白酶抑制剂混合物已针对组蛋白标记蛋白进行了优化和测试

制备说明

该产品在DMSO中是澄清溶液。 推荐1mL溶液可以抑制20gE. Coli细胞或10g细胞或10g 杆状病毒感染细胞得到的100ml细胞裂解液中的蛋白酶活性。

Safety Information

• 储存分类代码: 10 - Combustible liquids
• WGK: WGK 1
• 闪点(F): 185.0 °F - closed cup
• 闪点(C): 85 °C - closed cup