Merck
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R4752

Sigma-Aldrich

Ribonucleic acid, transfer from bovine liver

Type XI, lyophilized powder

Synonym(s):
Transfer RNA, tRNA
CAS Number:
MDL编号:

类型

Type XI

质量水平

200

形式

lyophilized powder

储存温度

−20°C

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一般描述

Transfer ribonucleic acid (tRNA) is a non-coding RNA. It is approximately 70-100 base pair long. It has a cloverleaf shaped secondary structure. It has three or four arms and a stem structure. It has minor bases like pseudouridine, dihydrouridine and methylated bases in its structure. It has a binding site for triplet nucleotide sequence and for amino acids.

应用

Ribonucleic acid, transfer from bovine liver has been used:
  • to analyze the efficiency of thioflavin T (ThT) as a fluorophore
  • to test its ability to inhibit the growth of endothelial cells
  • as a component in translation mixture
  • as a non-specific competitor RNA for protein purification studies using poly(A) tailed RNA affinity column

Ribonucleic acid, transfer from bovine liver may be used as a starting material for the purification of specific amino acyl-tRNA species by methods such as liquid column chromatography or high-performance liquid chromatography (HPLC).

包装

50, 100 units in serum bottle

生化/生理作用

Transfer ribonucleic acid (tRNA) plays a major role in translation, a key step in protein synthesis. It decodes mRNA into protein. There are about 40-60 different tRNAs in a eukaryotic cell and fewer in bacteria. This depends on the degeneracy of the amino acid code. Each amino acid has a specific tRNA. tRNAs are charged with specific amino acid and carries them to the site of protein synthesis. It binds to the ribosome and directs the amino acid to the growing polypeptide chain. It also functions as a regulator of various biological processes. Mutations associated with tRNA is implicated in human diseases.

单位定义

一单位在 1.0mL 水(1cm 光程)中的 A260 为 1.0。

分析说明

This product is evaluated for amino acceptor activity using aminoacyl-tRNA synthetase and L-arginine

储存分类代码

13 - Non Combustible Solids

WGK

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

Certificate of Analysis

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Certificate of Origin

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