转移 RNA (tRNA) 是从面包酵母中分离得到的。
Transfer RNA (tRNA) is a short nucleotide RNA chain, which has an L-shaped structure.
Ribonucleic acid, transfer from baker′s yeast (S. cerevisiae) has been used to precipitate mRNA of denuded oocytes and for reverse transcription. It has also been used to seal magnetic beads in RNA pull-down assay of nasopharyngeal carcinoma (NPC) cells.
Transfer RNA (tRNA) acts as an ‘adaptor′ molecule that translates the three-nucleotide codon sequence in the mRNA to the corresponding amino acid of that codon. It determines the genetic code by linking amino acids and nucleic acids. tRNA is involved in viral replication, bacterial cell wall biosynthesis, cell stress response, and animal behavior control. Aberrations in tRNA molecules are associated with several human diseases.
tRNA 溶于 1 mM EDTA 的 10 mM Tris HCl (pH 7.4) 溶液中。