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RIP

Sigma-Aldrich

Imprint® RNA免疫沉淀试剂盒

High-capacity Protein A magnetic beads for successful RNA Immunoprecipitation,suitable for use with mRNA and microRNA

Synonym(s):
Magnetic Bead RNA Immunoprecipitation, RNA Immunopreciptation, mRNA Immunopreciptation, microRNA Immunoprecipitation
NACRES:
NA.54

质量水平

200

Related Categories

一般描述

RNA免疫沉淀(RIP)是研究核糖核蛋白(RNP)复合物中RNA结合蛋白(RBP)及其RNA靶点的强大方法。抗特定RBP的抗体被用于共沉淀RNP,即RBP及其RNA伴侣。然后,可以通过下一代测序鉴定RNA或通过RT-PCR检测特定RNA。
当与合适的抗体联用时,Imprint RNA免疫沉淀试剂盒可提供RIP实验成功所需的所有试剂。该试剂盒包括两种裂解缓冲液:一种较为温和,与RNP结合不紧密;另一种则很苛刻,可与RNP密切结合。其中随附蛋白A磁珠,用于收集免疫沉淀物,方便洗涤。该试剂盒还包括用于阴性对照RIP的兔和小鼠IgG,以及一种兔抗鼠的桥接抗体,用于将小鼠单克隆一抗有效结合至磁珠上的蛋白A;其中还含有蛋白酶和核糖核酸酶抑制剂,可保护RIP实验过程中RNP不被降解。

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应用

Imprint® RNA免疫沉淀试剂盒已可用于:
  • 适用于下游应用
  • 从个体靶标表征到全基因组图谱分析技术
  • 信号转导途径的表征
  • 验证ChIp-chIP和ChIP-seq数据

特点和优势

  • 更高结合能力的Xtra蛋白A磁珠
  • 细胞裂解和免疫沉淀的分步实验方案
  • 包含RNA酶抑制剂和无RNA酶试剂,以保持RNA的完整性
  • 内附阴性对照,且包含抗体

法律信息

Imprint is a registered trademark of Sigma-Aldrich Co. LLC

Kit Components Also Available Separately

Product No.
Description
SDS

  • I5381IgG from mouse serum, reagent grade, ≥95% (SDS-PAGE), lyophilized powder

  • I5006IgG from rabbit serum, reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powder

  • M7023Anti-Mouse IgG (whole molecule) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution

  • P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution

  • R1158Ribonuclease Inhibitor, recombinant, expressed in E. coli, 20-40 units/μL

象形图

FlameExclamation mark

警示用语:

Warning

预防措施声明

危险分类

Eye Irrit. 2 - Flam. Liq. 3 - Skin Irrit. 2 - STOT SE 3

储存分类代码

3 - Flammable liquids

闪点(F)

100.4 °F

闪点(C)

38 °C

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

SDS

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