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S8830
SIGMAFAST ™蛋白酶抑制剂混合物片剂，无EDTA
for use in purification of Histidine-tagged proteins
All Photos(2)
Synonym(s):
Protease Inhibitor Cocktail
MDL编号:
NACRES:
NA.77
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一般描述
SIGMAFAST™蛋白酶抑制剂混合物片剂不含EDTA，是一种片剂制剂，其中含有水溶性蛋白酶抑制剂，可广泛特异性抑制丝氨酸、半胱氨酸、天冬氨酸和金属蛋白酶。该片剂兼容固定化金属离子亲和层析(IMAC)处理样品，如组氨酸标记蛋白纯化样品。该片剂不含可能干扰IMAC处理的强金属螯合剂，如EDTA。 最后，该片剂含有独特的专有配方（包括胃抑素A盐），可提供片剂形式通常无法提供的额外保护作用。
特异性
抑制丝氨酸、半胱氨酸、天冬氨酸和金属蛋白酶。
应用
其提供的广泛保护功能使SIGMAFAST™ 蛋白酶抑制剂混合物片剂可用于纯化组氨酸标记蛋白质，用作通用蛋白酶抑制剂混合物和细胞裂解缓冲液成分。该片剂还经过优化和测试，可与HIS-Select介质配合使用。
包装
2, 20 tablets
特点和优势
EDTA-Free: Contains no strong metal chelators, such as EDTA, which might interfere with IMAC processing.
Pepstatin A: Contains a unique, proprietary formulation that includes a pepstatin A salt for additional protection not normally obtained in a tablet format.
Broad Specificity: Inhibits serine, cysteine, aspartic, and metalloproteases, providing broad protection for protein samples.
High Yield: One tablet makes 100 mL of cocktail, and is recommended for the inhibition of the proteases present in a maximum of 20 g of cell mass in 100 mL of extract.
Long Stability: The tablets are stable as supplied for at least 2 years at 2–8 °C, and the reconstituted protease inhibitor solution (1´ or 10´) is stable for at least 2 weeks at 2–8 °C.
Pepstatin A: Contains a unique, proprietary formulation that includes a pepstatin A salt for additional protection not normally obtained in a tablet format.
Broad Specificity: Inhibits serine, cysteine, aspartic, and metalloproteases, providing broad protection for protein samples.
High Yield: One tablet makes 100 mL of cocktail, and is recommended for the inhibition of the proteases present in a maximum of 20 g of cell mass in 100 mL of extract.
Long Stability: The tablets are stable as supplied for at least 2 years at 2–8 °C, and the reconstituted protease inhibitor solution (1´ or 10´) is stable for at least 2 weeks at 2–8 °C.
组分
AEBSF
乌苯美司
E-64
抑肽素A
磷阿米酮
亮抑酶肽
抑酶肽
乌苯美司
E-64
抑肽素A
磷阿米酮
亮抑酶肽
抑酶肽
数量
一片可制备100 mL混合液。 推荐将一片用于抑制100 mL提取物中最多20 g细胞质量中存在的蛋白酶。
外形
片剂是可溶于水的
储存及稳定性
该片剂在2-8°C温度下至少可稳定保存2年。重悬的蛋白酶抑制剂溶液（1´或10´）在2–8°C下至少可稳定保存2周。
法律信息
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC
警示用语：
Warning
危险声明
危险分类
Eye Irrit. 2 - Skin Irrit. 2
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(F)
Not applicable
闪点(C)
Not applicable
个人防护装备
Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges
Certificate of Analysis
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Certificate of Origin
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Data Sheets
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