所有图片(5)

SEQXE

Sigma-Aldrich

SeqPlex DNA扩增试剂盒

For use with high throughput sequencing technologies, Whole Genome Amplification kit designed to facilitate Next Gen Sequencing.

别名:
SeqPlex增强型DNA扩增试剂盒
NACRES:
NA.55

质量水平

200

technique(s)

whole genome amplification: suitable

运输

wet ice

储存温度

−20°C

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一般描述

用于全基因组扩增(WGA)的SeqPlex增强型DNA扩增试剂盒,专门针对极小量或降解/高度片段化的DNA进行二代测序(NGS)。染色质免疫沉淀(ChIP)或福尔马林固定的石蜡包埋组织样品(FFPE)的DNA含量,通常不能满足成功制备二代测序文库的需求。SeqPlex试剂盒可对这类DNA以及其他用于NGS的小量/高度片段化DNA样品进行预扩增。该试剂盒是WGA产品线的延伸产品,已被整合到Illumina®、SOLiD454测序工作流程中。

应用

SeqPlex DNA Amplification Kit has been used for whole genome amplification.

特点和优势

  • 采用随机引物技术扩增片段化DNA,如ChIP或FFPE
  • 有助于对低至100 pg的ChIP DNA进行测序
  • 采用增强型引物,可获得完整的基因组覆盖、最低的序列偏差、引物清除以及适用于二代测序的扩增子大小
  • 可兼容用于二代测序的Illumina®、SOLiD或454文库制备

其他说明

SEQXE-500RXN 是按需生产。获取更多信息,请发邮件至techserv@sial.com 联系技术服务。

法律信息

Illumina is a registered trademark of Illumina, Inc.
SOLiD is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
iCAT is a registered trademark of University of Washington

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书

  • W4502Water, Nuclease-Free Water, for Molecular Biology

  • Library Preparation Buffer

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable

分析证书

原产地证书 (CofO)

Syuzo Kaneko et al.
Cancers, 13(9) (2021-05-01)
Although chromatin immunoprecipitation and next-generation sequencing (ChIP-seq) using formalin-fixed paraffin-embedded tissue (FFPE) has been reported, it remained elusive whether they retained accurate transcription factor binding. Here, we developed a method to identify the binding sites of the insulator transcription factor
Isolation and sequencing of active origins of DNA replication by nascent strand capture and release (NSCR).
Kunnev D, et al.
Journal of biological methods, 2(4) (2015)
Xiang Yu et al.
Genome research, 27(7), 1238-1249 (2017-04-08)
Type II topoisomerases orchestrate proper DNA topology, and they are the targets of anti-cancer drugs that cause treatment-related leukemias with balanced translocations. Here, we develop a high-throughput sequencing technology to define TOP2 cleavage sites at single-base precision, and use the
Dimiter Kunnev et al.
Journal of biological methods, 2(4) (2015-01-01)
Nascent strand capture and release (NSCR) is a method for isolation of short nascent strands to identify origins of DNA replication. The protocol provided involves isolation of total DNA, denaturation, size fractionation on a sucrose gradient, 5'-biotinylation of the appropriate
Takuya Hayakawa et al.
Cytogenetic and genome research, 161(8-9), 437-444 (2021-11-25)
E/L Repli-seq is a powerful tool for detecting cell type-specific replication landscapes in mammalian cells, but its potential to monitor DNA replication under replication stress awaits better understanding. Here, we used E/L Repli-seq to examine the temporal order of DNA

相关内容

SeqPlex 增强型DNA扩增试剂盒 (SEQXE)实验方案

面向全基因组扩增(WGA)的SeqPlexDNA扩增试剂盒(SEQXE)专用于对极小量或降解/高度片段化的DNA进行下一代测序(NGS)。

SeqPlex Enhanced DNA Amplification Kit (SEQXE) Protocol

The SeqPlex DNA Amplification Kit for whole genome amplification (WGA) is designed to facilitate next-generation sequencing (NGS) from extremely small quantities or from degraded/highly fragmented DNA

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Imprint®染色质免疫沉淀试剂盒(CHP1)实验方案

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