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STR1

Sigma-Aldrich

增强型禽类第一链合成试剂盒

Components for cDNA synthesis with enhanced AMV reverse transcriptase

NACRES:
NA.55

质量水平

200

用途

sufficient for 50 reactions
1 kit sufficient for 50 reactions

特点

dNTPs included
hotstart: no

应用

RT-PCR: suitable

颜色

colorless

input

purified RNA

运输

dry ice

储存温度

−20°C

应用

增强的 Avian第一链合成试剂盒,应用了一款高度纯化的禽成髓细胞瘤病毒逆转录酶(eAMV-RT),后者的反应性能远超标准型AMV-RT。这一高效eAMV-RT在高温(高达65 °C)能够下可打开RNA的复杂二级结构,是以总RNA或poly(A)+ RNA为模板 制备高质量全长cDNA的理想选择。

单位定义

一单位酶可以多聚腺苷酸为模板,以12-18长度的oligo(dT)为引物,在10分钟内将1纳摩TMP整合进TCA-沉淀材料中。

法律信息

Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.

仅试剂盒组分

产品编号
说明

  • Enhanced Avian Reverse Transcriptase 1000 U

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书

  • Deoxynucleotide mix 50 μL

  • O4387Anchored oligo (dT)23 100 μL

  • R7647Random nonamers 100 μL

  • O4387Ribonuclease inhibitor 50 μL

  • W1754PCR grade water 1.5 mL

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable

分析证书

原产地证书 (CofO)

Julio Carrion et al.
Journal of immunology (Baltimore, Md. : 1950), 189(6), 3178-3187 (2012-08-15)
The low-grade oral infection chronic periodontitis (CP) has been implicated in coronary artery disease risk, but the mechanisms are unclear. In this study, a pathophysiological role for blood dendritic cells (DCs) in systemic dissemination of oral mucosal pathogens to atherosclerotic
María José Martín et al.
The Journal of biological chemistry, 282(47), 34510-34524 (2007-09-21)
Heme oxygenase-1 (HO-1), an inducible enzyme that metabolizes the heme group, is highly expressed in human Kaposi sarcoma lesions. Its expression is up-regulated by the G protein-coupled receptor from the Kaposi sarcoma-associated herpes virus (vGPCR). Although recent evidence shows that
Aerosol-administered α-tocopherol attenuates lung inflammation in rats given lipopolysaccharide intratracheally.
Experimental Lung Research, 31, 34510-34524 (2007)
Sigma-Aldrich Corporation's Life Science Quarterly. Hot Start RT-PCR results in improved performance of the enhanced Avian RT-PCR
Easlund, E., and Mueller, E.
Sigma data, 2-5 (2001)
E M Brooks et al.
BioTechniques, 19(5), 806-812 (1995-11-01)
The secondary structure in mRNA is essential for many processes, but it can present a technical problem in making full-length cDNA with reverse transcriptases. Furthermore, different reverse transcriptases have differing abilities to transcribe through regions with secondary structure, which can

实验方案

The 3'/5' Assay for Analysis of RNA Integrity Protocol

The 3’/5’ integrity assay is a potential first step in the identification of RNA degradation. The assay is particularly useful when a large number of samples are to be analyzed or when the degradation is less than that detected by capillary systems but still sufficient to effect qPCR analyses.

相关内容

PCR的应用

聚合酶链式反应(PCR)是一种扩增核酸分子的技术,广泛应用于逆转录PCR、热启动PCR、终点PCR等实验中。

PCR Applications

Polymerase chain reaction (PCR) is a technique for amplifying nucleic acid molecules and is commonly used in many applications, including RT-PCR, hot start PCR, end point PCR and more.

逆转录

分析基因表达的方法之一是测量基因的mRNA浓度。此类分析面临着若干挑战,例如不同转录本之间的半衰期不同、转录时间模式以及mRNA和蛋白质之间缺乏相关性。

Reverse Transcription

One approach to the analysis of gene expression is to measure the concentration of mRNA of a gene. There are several challenges to such analyses, such as the differences in half life between different transcripts, the temporal patterns of transcription and the lack of correlation between mRNA and protein.

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