Merck
  • T1063
All Photos(1)

T1063

Sigma-Aldrich

Thrombin 来源于人类血浆

lyophilized powder, ≥2800 NIH units/mg protein (E1%/280, 18.3)

Synonym(s):
IIa因子
CAS Number:
Enzyme Commission number:
EC 号:
MDL编号:
NACRES:
NA.54

形式

lyophilized powder

质量水平

specific activity

≥2800 NIH units/mg protein (E1%/280, 18.3)

分子量

37.4 kDa

杂质

HIV, hepatitis B and hepatitis C, tested negative

UniProt登记号

application(s)

diagnostic assay manufacturing

储存温度

−20°C

Gene Information

human ... F2(2147)

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This Item
T6884T7513T6634
form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

specific activity

≥2800 NIH units/mg protein (E1%/280, 18.3)

specific activity

≥2,000 NIH units/mg protein (E1%/280, 18.3)

specific activity

≥2,000 NIH units/mg protein (E1%/280 = 19.5)

specific activity

600-2,000 NIH units/mg protein (biuret)

mol wt

37.4 kDa

mol wt

37.4 kDa

mol wt

heavy chain ~33 kDa, light chain ~5 kDa

mol wt

heavy chain ~33 kDa, light chain ~5 kDa

impurities

HIV, hepatitis B and hepatitis C, tested negative

impurities

HIV and HBsAg, source material tested negative

impurities

-

impurities

-

UniProt accession no.

P00734

UniProt accession no.

P00734

UniProt accession no.

P00735

UniProt accession no.

P00735

application(s)

diagnostic assay manufacturing

application(s)

-

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

一般描述

凝血酶是止血过程中的最终凝血蛋白酶,可同时促进促凝血和抗凝血作用。

应用

凝血酶被用于重组融合蛋白的位点特异性切割,这些重组融合蛋白具有可接近的凝血酶识别位点以除去亲和标签。凝血酶已可用于评估低分子量肝素在 体外 对肝硬化患者在肝病不同阶段的凝血酶生成的影响。

生化/生理作用

选择性裂解纤维蛋白原中 Arg-Gly 键形成纤维蛋白和纤维蛋白肽 A 和 B 的丝氨酸蛋白酶。

单位定义

活性以NIH单位表示,它是通过与NIH凝血酶参考标准品直接对比而获得的

外形

冻干品来自于0.02 M Bis/Tris缓冲液,pH 6.5,含0.15M氯化钠和0.1% PEG-8000

分析说明

NIH 试验步骤基于 Biggs 方法的改进,使用 0.2mL 稀释血浆(与生理盐水 1:1 混合,作为底物)和 0.1mL Biggs 凝血酶样品(稳定于 1% 缓冲白蛋白溶液中)。只使用 15-25 秒之间的凝血时间分析凝血酶浓度。

其他说明

象形图

Exclamation markHealth hazard

警示用语:

Danger

危险分类

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

储存分类代码

11 - Combustible Solids

WGK

WGK 2

闪点(F)

Not applicable

闪点(C)

Not applicable


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