Merck
All Photos(5)

T1428

Sigma-Aldrich

脱铁-转铁蛋白 牛

BioReagent, suitable for cell culture, ≥98%

Synonym(s):
Human serotransferrin
CAS Number:
EC 号:
MDL编号:
NACRES:
NA.83

生物来源

bovine

质量水平

产品线

BioReagent

检测方案

≥98%

形式

powder

technique(s)

cell culture | mammalian: suitable

杂质

iron, essentially free

溶解性

H2O: 10 mg/mL

UniProt登记号

储存温度

2-8°C

Gene Information

bovine ... TF(280705)

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This Item
T5391T2252T4382
Sigma-Aldrich

Sigma-Aldrich

T5391

脱铁转铁蛋白 人

apo-Transferrin human ≥95% protein basis (biuret)

Sigma-Aldrich

T2252

脱铁转铁蛋白 人

apo-Transferrin human powder, ≥98% (agarose gel electrophoresis)

Sigma-Aldrich

T4382

脱铁转铁蛋白 人

biological source

bovine

biological source

-

biological source

human

biological source

human

assay

≥98%

assay

≥98%

assay

≥95% protein basis (biuret), ≥98% (agarose gel electrophoresis)

assay

≥98% (agarose gel electrophoresis)

form

powder

form

powder

form

powder

form

powder

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

impurities

iron, essentially free

impurities

HIV and HBsAg, source material tested negative, iron, essentially free

impurities

-

impurities

HIV and HBsAg, source material tested negative

solubility

H2O: 10 mg/mL

solubility

H2O: 50 mg/mL

solubility

H2O: 50 mg/mL

solubility

H2O: 50 mg/mL

一般描述

脱铁转铁蛋白是一种无铁形式的转铁蛋白,存在于循环系统中。

应用

所有细胞都需要铁才能从其环境中适当吸收氧气。 转铁蛋白是一种从血清中分离出来的蛋白质,可引起铁从培养基中摄取并转运到细胞中。 这允许适当的氧气吸收,并且还刺激与生长相关的酶活性。 铁饱和转铁蛋白(全转铁蛋白)在释放铁后不断循环,变成缺铁转铁蛋白(脱辅基转铁蛋白)。 选择使用哪种形式取决于培养条件。 在诸如含有高水平铁盐的Dulbecco′s改良的Eagle′s培养基/Ham′s营养混合物F12(DMEM/F12)的培养基中,优选使用脱铁转铁蛋白。
牛载脂蛋白转铁蛋白已用作以下材料的组分:
  • 牛乳腺上皮细胞培养的基础培养基
  • 精原干细胞培养的最低必需培养基α(MEM-α)
  • 间充质干细胞培养的诱导培养基

生化/生理作用

脱铁转铁蛋白(apoTf)有助于防止铁在组织中积聚,特别是在非输血依赖型β地中海贫血中。

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


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