Reliable cell culture product that gets frequent use
T3924
胰蛋白酶-EDTA 溶液
1 ×, sterile; sterile-filtered, BioReagent, suitable for cell culture, 0.5 g porcine trypsin and 0.2 g EDTA, 4Na per liter of Hanks′ Balanced Salt Solution with phenol red
Trypsin-EDTA solution
1 of 2 reviewers received a sample product or took part in a promotion
别名:
Cell dissociation enzyme
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选择尺寸
变更视图
| 包装规格 | 货号 | 是否有货 | 价格 |
|---|---|---|---|
| 100 mL | 预计发货时间2026年4月06日发货地SIGMA ALDRICH Pte Ltd | $40.00 | |
| 500 mL | 预计发货时间2026年4月06日发货地SIGMA ALDRICH Pte Ltd | $134.00 |
关于此项目
Biological source:
Porcine
Concentration:
1 ×
biological source
Porcine
sterility
sterile; sterile-filtered
product line
BioReagent
form
solution
mol wt
23.4 kDa
concentration
1 ×
technique(s)
cell culture | mammalian: suitable, single cell analysis: suitable
impurities
Porcine parvovirus, none detected (9 CFR)
pH
7.0-8.0
shipped in
dry ice
storage temp.
−20°C
Application
该产品的典型用途是使黏附细胞从培养表面脱落。 使细胞从附着面脱落所需的胰蛋白酶浓度主要取决于细胞类型和培养阶段。
适合用于制备测序用单细胞悬液。
适合用于制备测序用单细胞悬液。
Biochem/physiol Actions
胰蛋白酶可切割赖氨酸和精氨酸残基的C末端肽段。 如果酸性残基位于切割位点的任意一侧，则该反应的水解速度减慢，并且如果脯氨酸残基位于切割位点的羧基侧，则水解停止。 胰蛋白酶活性的最佳pH条件为7-9。 胰蛋白酶还可以切割氨基酸合成衍生物的酯和酰胺键。 将EDTA添加到胰蛋白酶溶液中作为螯合剂，用于中和钙离子和镁离子以防止其抑制胰蛋白酶活性。 去除这些离子会增加酶活性。
丝氨酸蛋白酶抑制剂（包括DFP、TLCK、APMSF、AEBSEF和抑肽酶等）会抑制胰蛋白酶。
丝氨酸蛋白酶抑制剂（包括DFP、TLCK、APMSF、AEBSEF和抑肽酶等）会抑制胰蛋白酶。
Preparation Note
本产品含有酚红。 由于在干冰上运输，包装中可能累积大量二氧化碳。 这些CO2可能会进入溶液并略微降低pH值，使溶液呈橙色而不是粉红色。 橙色溶液仍然可以使用，或者可以用氢氧化钠调节pH。 使用高浓度胰蛋白酶长时间孵育细胞，会破坏细胞膜并杀死细胞。 溶解胰蛋白酶或稀释浓缩胰蛋白酶溶液时，应使用不含Ca2+ 或Mg2+的缓冲盐溶液。
Other Notes
胰蛋白酶是由含223个氨基酸残基的单链多肽组成的，它通过切割胰蛋白酶原的Lys-1le肽键以去除N-末端六肽而获得。 氨基酸序列通过6个二硫键交联。 这是天然形式的胰蛋白酶——β-胰蛋白酶。 β-胰蛋白酶可以自溶，在Lys-Ser残基处断裂，产生α-胰蛋白酶。 胰蛋白酶是丝氨酸蛋白酶家族的成员。
Disclaimer
本品应置于-10至-40°C环境冷冻储存。避免反复冻融。
1 of 1
此商品 | |||
|---|---|---|---|
| biological source Porcine | biological source Porcine | biological source Porcine | biological source Porcine pancreas |
| technique(s) cell culture | mammalian: suitable, single cell analysis: suitable | technique(s) cell culture | mammalian: suitable, single cell analysis: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| form solution | form solution | form solution | form solution |
| concentration 1 × | concentration 0.25% | concentration 10 × | concentration 1 × |
| pH 7.0-9.0 | pH 7.0-7.6 | pH 7.0-7.6 | pH 7.0-7.6 |
| mol wt 23.4 kDa | mol wt 23.4 kDa | mol wt 23.4 kDa | mol wt - |
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
实验方案
Technical information for working with porcine aortic endothelial cells including thawing, subculturing and cryopreservation
Technical information for working with human aortic smooth muscle cells including thawing, subculturing and cryopreservation.
Technical information for working with human aortic smooth muscle cells including thawing, subculturing and cryopreservation.
商品
Technical information for working with porcine coronary artery endothelial cells including thawing, subculturing and cryopreservation
Technical information for working with porcine pulmonary artery endothelial cells including thawing, subculturing and cryopreservation
Technical information for working with human coronary artery smooth muscle cells including thawing, subculturing and cryopreservation
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| T3924-100ML | 04061838260048 |
| T3924-500ML | 04061838260055 |