Merck
  • T7009
All Photos(1)

T7009

Sigma-Aldrich

Thrombin 来源于人类血浆

lyophilized powder, ≥1,000 NIH units/mg protein (E1%/280, 18.3)

Synonym(s):
IIa因子
CAS Number:
Enzyme Commission number:
EC 号:
MDL编号:
NACRES:
NA.54

形式

lyophilized powder

质量水平

specific activity

≥1,000 NIH units/mg protein (E1%/280, 18.3)

分子量

37.4 kDa

杂质

HIV and HBsAg, source material tested negative

UniProt登记号

application(s)

diagnostic assay manufacturing

储存温度

−20°C

Gene Information

human ... F2(2147)

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This Item
T4393T6884T1063
form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

specific activity

≥1,000 NIH units/mg protein (E1%/280, 18.3)

specific activity

1500-3500 NIH units/mg protein (E1%/280, 18.3)

specific activity

≥2,000 NIH units/mg protein (E1%/280, 18.3)

specific activity

≥2800 NIH units/mg protein (E1%/280, 18.3)

mol wt

37.4 kDa

mol wt

-

mol wt

37.4 kDa

mol wt

37.4 kDa

impurities

HIV and HBsAg, source material tested negative

impurities

HIV, hepatitis B and hepatitis C, tested negative

impurities

HIV and HBsAg, source material tested negative

impurities

HIV, hepatitis B and hepatitis C, tested negative

UniProt accession no.

P00734

UniProt accession no.

P00734

UniProt accession no.

P00734

UniProt accession no.

P00734

application(s)

diagnostic assay manufacturing

application(s)

-

application(s)

-

application(s)

diagnostic assay manufacturing

一般描述

F2基因编码凝血酶蛋白,对应于人类染色体11p11.2。

应用

人血浆凝血酶应用:
  • 溶解在CaCl2 中,并添加到聚乙二醇化的纤维蛋白原/细胞溶液中以生产纤维蛋白
  • 在孔板上聚合纤维蛋白
  • 用作蛋白酶激活受体的内源性激动剂蛋白酶:刺激细胞的PAR1、PAR3和PAR4

生化/生理作用

凝血酶是一种内源性丝氨酸蛋白酶,可刺激产生血管损伤诱导的白细胞、血小板、内皮细胞和间充质细胞。这一作用通过G蛋白偶联受体介导。它可催化纤维蛋白原在Arg−Gly键处断裂,形成纤维蛋白并释放纤维蛋白肽A和B。凝血酶在体内的主要形式是在肝脏中产生的酶原凝血酶原(II因子)。凝血酶原是凝血激活因子,高含量预示存在癌症的可能。凝血酶可用于切割融合蛋白。融合蛋白切割以凝血酶:融合蛋白1:500的比例进行。
选择性裂解纤维蛋白原中 Arg-Gly 键形成纤维蛋白和纤维蛋白肽 A 和 B 的丝氨酸蛋白酶。

单位定义

直接与NIH凝血酶参考标准比较而得到NIH单位表示的活性

外形

由生理盐水柠檬酸钠缓冲液,pH 6.5,制得冻干粉

分析说明

NIH 试验步骤基于 Biggs 方法的改进,使用 0.2mL 稀释血浆(与生理盐水 1:1 混合,作为底物)和 0.1mL Biggs 凝血酶样品(稳定于 1% 缓冲白蛋白溶液中)。只使用 15-25 秒之间的凝血时间分析凝血酶浓度。

其他说明

象形图

Health hazard

警示用语:

Danger

危险声明

预防措施声明

危险分类

Resp. Sens. 1

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable


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