Human transferrin has an approximate molecular weight range of 76-81 kDa. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/418/013/t8158pis.pdf
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此商品 | T3309 | T3915 | 616397-M |
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | stem cell: suitable | technique(s) cell culture | mammalian: suitable | technique(s) - |
| form powder | form powder | form powder | form lyophilized solid |
| shipped in ambient | shipped in - | shipped in - | shipped in - |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C |
| solubility H2O: 50 mg/mL | solubility - | solubility - | solubility water: 10 mg/mL |
| impurities endotoxin, tested, hepatitis and HIV antibody, none detected | impurities ≤1.0 EU/mg endotoxin | impurities - | impurities - |
一般說明
转铁蛋白是具有分支糖链的糖蛋白，多肽链中具有两个铁离子结合位点。[1]
生化／生理作用
準備報告
60℃热处理10小时。
分析報告
通过琼脂糖电泳纯化。
其他說明
部分铁饱和
免責聲明
供研究使用。在法国，在用于科研目的时（包括进口和出口活动（《公共健康法》第L 1211-1条，第2节））该产品为管制品。购买者（即最终用户）需要获得《公共健康法》法第L 1211-1条规定的法国研究部的进口授权。订购本产品即表明您确认已经获得合适的进口授权。
相關產品
产品编号
说明
价格
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Non-transferrin bound iron: a key role in iron overload and iron toxicity
Brissot P, et al.
Biochim. Biophys. Acta Gen. Subj., 1820(3), 403-410 (2012)
Cells: Molecules and Mechanisms, 174-174 (2009)
Martijn F Hoes et al.
European journal of heart failure, 20(5), 910-919 (2018-02-28)
Iron deficiency is common in patients with heart failure and associated with a poor cardiac function and higher mortality. How iron deficiency impairs cardiac function on a cellular level in the human setting is unknown. This study aims to determine
Detection of N-glycans on small amounts of glycoproteins in tissue samples and sodium dodecyl sulfate-polyacrylamide gels
Yoshimura T, et al.
Analytical Biochemistry, 423(2), 253-260 (2012)
Eun-Min Kim et al.
PLoS neglected tropical diseases, 13(4), e0006843-e0006843 (2019-04-04)
Clonorchis sinensis is a group I bio-carcinogen responsible for cholangiocarcinoma (CHCA) in humans. However, the mechanism by which C. sinensis promotes carcinogenesis is unclear. Using the human cholangiocyte line H69, we investigated cell proliferation and gap junction protein expression after
商品
转铁蛋白和其他细胞培养成分如何影响无血清、无蛋白细胞培养系统（用于生物制造包括单克隆抗体在内的异源蛋白）的性能。本页介绍转铁蛋白的体外化学和生物化学。
Understand how transferrin affects serum-free cell culture systems, crucial for biomanufacturing heterologous proteins like monoclonal antibodies.
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I want to know the Molecular Weight of this product.
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What is the correct method for storing item T8158, Human Transferrin, after reconstitution?
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After reconstitution, it is advisable to aliquot and freeze the product T8158, Human Transferrin at -20°C for long-term storage or add NaN₃ and refrigerate it at 4°C for short-term storage. Stock solutions can remain stable for up to 1 year at -20°C or for up to 2 weeks at 4°C.
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We were wondering how to store the dissolved transferrin. We have frozen it at -80. If we thaw it can we can freeze it again,
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Transferrin is fairly stable in solution. Non-sterile aqueous solutions may be stored at 4 °C for 5-10 days. Solutions may be filter-sterilized to extend solution stability. Various media supplements containing transferrin, such as ITS (Insulin/Transferrin/Selenium), are stable for up to 30 days at 4 °C and may be stored in frozen aliquots at -20 °C. for up to one year. Avoid repeated freeze/thaw cycles as this may contribute to precipitation occurring.
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How is this protein produced and what is the rest 2% impurity? Thanks!
1 answer-
The preparation method is considered proprietary. The residual impurities have not been identified. This product is tested for endotoxin and iron. Please see the Sample Certificates of Analysis:
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/T8158-BULKBCCK4250.pdfHelpful?
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What is the carbohydrate content of human transferrin?
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We have found the information on transferrin in a reference book:The Plasma Proteins, F.W. Putnam, ed, volume 2, chapter 4, Table II.According to the table, Transferrin is 6 percent carbohydrate, citing G.A. Jamieson, J. Biol. Chem. 240(7), 2914-2920 (1965).The table also gives information on the percentage of each type of monosaccharide that is present.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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