所有图片(1)

T8787

Sigma-Aldrich

Triton X-100

for molecular biology

别名:
4-(1,1,3,3-四甲基丁基)苯基-聚乙二醇, 叔辛基苯氧基聚乙烯乙氧基乙醇, 聚乙二醇叔辛基苯基醚
线性分子式:
t-Oct-C6H4-(OCH2CH2)xOH, x= 9-10
CAS号:
Beilstein:
2315025
MDL编号:
PubChem化学物质编号:
NACRES:
NA.21

质量水平

200

等级

for molecular biology

描述

non-ionic

形式

liquid

分子量

micellar avg mol wt 80,000
average mol wt 625

聚集数

100-155

应用

hematology: suitable
histology: suitable
protein purification: suitable
protein quantification: suitable
western blot: suitable

pH

9.7

CMC

0.2-0.9 mM (20-25°C)

mp

6 °C

转变温度

cloud point 65 °C
pour point ~7 °C

溶解性

water: 0.1 mL/mL, clear to slightly hazy, colorless to faintly yellow

密度

1.06 g/mL at 25 °C (lit.)

HLB

13.5

特色行业

Diagnostic Assay Manufacturing

异质活性

DNase and RNase, none detected

储存温度

room temp

SMILES string

CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1

InChI

1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3

InChI key

HNLXNOZHXNSSPN-UHFFFAOYSA-N

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相关类别

一般描述

Triton X-100是一种常用的非离子型的表面活性剂和乳化剂,经常用于生化应用以溶解蛋白质。它被视为一种不变性的相对温和的去污剂,并且在许多参考文献中作为一种常规被添加的试剂而被报道。它用于裂解细胞以提取蛋白和细胞器。它还可通透活细胞膜用于转染。

应用

Triton X-100已用于:
  • 免疫组化用于平面视网膜的染色
  • 与冰冷的PBS(磷酸盐缓冲液)一起悬浮细胞用于细胞DNA分析和Annexin V检测
  • 在免疫荧光显微研究中对细胞进行通透化处理
  • 在LDH检测中作为阳性对照用于测定细胞膜的完整性
  • 采用改良的Belfrage和Vaughan放射性酶法过程测定肝素后血浆中的脂肪酶活性
  • 外层膜蛋白提取物的制备
  • 作为提取缓冲液中一种成分与tris-HCl、NaCl、CaCl2、ZnCl2、Brij 35一起用于小鼠肺细胞的匀浆
  • 免疫荧光标记的组织切片处理

包装

50, 100, 250 mL in poly bottle

生化/生理作用

广泛使用的非离子表面活性剂,用于在温和的非变性条件下进行膜恢复。

法律信息

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

分析证书

原产地证书 (CofO)

M A Ferguson et al.
Journal of applied physiology (Bethesda, Md. : 1985), 85(3), 1169-1174 (1998-09-08)
The purpose of this study was to determine the threshold of exercise energy expenditure necessary to change blood lipid and lipoprotein concentrations and lipoprotein lipase activity (LPLA) in healthy, trained men. On different days, 11 men (age, 26.7 +/- 6.1...
Jean-Sébastien Joyal et al.
Blood, 117(22), 6024-6035 (2011-03-01)
The failure of blood vessels to revascularize ischemic neural tissue represents a significant challenge for vascular biology. Examples include proliferative retinopathies (PRs) such as retinopathy of prematurity and proliferative diabetic retinopathy, which are the leading causes of blindness in children...
Effects of four different single exercise sessions on lipids, lipoproteins, and lipoprotein lipase.
Ferguson M A, et al.
Journal of Applied Physics, 85(3), 1169-1174 (1998)
Eric Soupene et al.
Experimental biology and medicine (Maywood, N.J.), 233(5), 507-521 (2008-04-01)
Acyl-CoA synthetase enzymes are essential for de novo lipid synthesis, fatty acid catabolism, and remodeling of membranes. Activation of fatty acids requires a two-step reaction catalyzed by these enzymes. In the first step, an acyl-AMP intermediate is formed from ATP....
Acrolein-activated matrix metalloproteinase 9 contributes to persistent mucin production.
Deshmukh H S, et al.
American Journal of Respiratory Cell and Molecular Biology, 38(4), 446-454 (2008)

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裂解和蛋白提取

常用的细胞裂解和蛋白质提取方法包括表面活性剂溶解法、冻融裂解法、渗透压休克法、超声破碎法、酶解细胞裂解法以及机械破碎技术(如Dounce、Polytron、研钵和研棒均质化)。

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