W1754

Sigma-Aldrich

PCR Reagent

线性分子式:
H2O
CAS号:
分子量:
18.02
Beilstein/REAXYS Number:
2050024
EC 号:
MDL编号:
PubChem化学物质编号:
NACRES:
NA.25

等级

PCR Reagent

质量水平

200

蒸汽密度

<1 (vs air)

蒸汽压

3 mmHg

无菌性

sterile-filtered

形式

liquid

包装

vial of 1.5 mL

应用

PCR: suitable

折射率

n20/D 1.34 (lit.)

pH值(酸碱度)

5-7

bp

100 °C (lit.)

mp

0 °C (lit.)

密度

1.000 g/mL at 3.98 °C (lit.)

异质活性

DNase, none detected
RNase, none detected

SMILES string

O

InChI

1S/H2O/h1H2

InChI key

XLYOFNOQVPJJNP-UHFFFAOYSA-N

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相关类别

一般描述

对 PCR 级水进行无菌过滤。它不含核酸外切酶(dna 酶、rna 酶)和核酸内切酶(切口酶),也不含核酸污染。

应用

聚合酶链反应 (PCR) 中用水补足样品的最终体积。

适用性

适用于聚合酶链式反应 (PCR)

其他说明

便于将水产品规格与 水规格表进行比较

storage_class_code

10 - Combustible liquids

WGK Germany

nwg

闪点(F)

No data available

闪点(C)

No data available

个人防护装备

Eyeshields, Gloves

J Loeffler et al.
Journal of clinical microbiology, 37(4), 1200-1202 (1999-03-13)
Successful in vitro amplification of fungal DNA in clinical specimens has been reported recently. In a collaboration among five European centers, the frequency and risk of contamination due to airborne spore inoculation or carryover contamination in fungal PCR were analyzed....
If you?ve seen one worm, have you seen them all? Spatial, community, and genetic variability of tubificid communities in Montana
Lodh N, et al.
Freshwater science, 34(3), 909-917 (2015)
Incidence and survival of non-O157 verocytotoxigenic Escherichia coli in soil
Bolton DJ, et al.
Journal of Applied Microbiology, 111(2), 484-490 (2011)
Multiplex PCR for rapid detection of genes encoding acquired metallo-beta-lactamases.
Matthew J Ellington et al.
The Journal of antimicrobial chemotherapy, 59(2), 321-322 (2006-12-23)
N Wellinghausen et al.
Applied and environmental microbiology, 67(9), 3985-3993 (2001-08-30)
Contamination of hospital water systems with legionellae is a well-known cause of nosocomial legionellosis. We describe a new real-time LightCycler PCR assay for quantitative determination of legionellae in potable water samples. Primers that amplify both a 386-bp fragment of the...
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Protocol using hot start dNTPs. Method includes modified nucleoside triphosphates that block DNA polymerase nucleotide incorporation during hot start PCR to increase specificity. Compatible with a variety of PCR reagents.
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Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures. AccuTaq LA.
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Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.
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