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Merck

W4502

Sigma-Aldrich

Water

suitable for electrophoresis, PCR

别名:

无DNase水, 无RNase水, 无核酸酶水, 无蛋白酶水, 未DEPC处理

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1 L
$181.00
10 X 50 ML
$226.00
6 X 1 L
$812.00
10 L
$842.00

$181.00


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选择尺寸

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1 L
$181.00
10 X 50 ML
$226.00
6 X 1 L
$812.00
10 L
$842.00

About This Item

线性分子式:
H2O
CAS号:
分子量:
18.02
Beilstein:
2050024
EC號碼:
MDL號碼:
分類程式碼代碼:
12191602
PubChem物質ID:
NACRES:
NA.31
技術:
PCR: suitable
electrophoresis: suitable
bp:
100 °C (lit.)
蒸汽壓力:
3 mmHg

$181.00


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获取大包装报价

产品名称

水, Nuclease-Free Water, for Molecular Biology

蒸汽密度

<1 (vs air)

品質等級

蒸汽壓力

3 mmHg

無菌

0.1 μm filtered

形狀

liquid

技術

PCR: suitable
electrophoresis: suitable

折射率

n20/D 1.34 (lit.)

pH值

5-7

bp

100 °C (lit.)

mp

0 °C (lit.)

密度

1.000 g/mL at 3.98 °C (lit.)

異物活動

DNase, RNase, Protease, free

SMILES 字串

O

InChI

1S/H2O/h1H2

InChI 密鑰

XLYOFNOQVPJJNP-UHFFFAOYSA-N

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應用

水已用于-
  • cDNA合成和PCR扩增[1][2]
  • 在qPCR检测前对瘤胃DNA样品进行稀释[3]
  • PCR(聚合酶链反应)和琼脂糖凝胶电泳[4]
  • RNA分离过程中RNA的洗脱[5]

特點和優勢

  • 无核酸酶水
  • 无DNase、RNase、蛋白酶
  • 未DEPC处理
  • 0.1 um过滤
  • 采用双蒸去离子水配制
  • 适于分子生物学应用

本品经过0.1um过滤。本品为分子生物学级试剂,适用于分子生物学应用。本产品针对核酸酶和蛋白酶的有无及微生物含量进行了分析,但未经焦碳酸二乙酯(DEPC)处理。

準備報告

该产品采用双蒸水和去离子水制备

其他說明

可根据水质参数表轻松比较产水参数。

相關產品

产品编号
说明
价格

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

nwg

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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其他客户在看

Presence of viral genomes in mineral water: a sufficient condition to assume infectious risk?
Gassilloud B et al
Applied and Environmental Microbiology, 69(7), 3965-3969 (2003)
Polymorphic exact tandem repeat A (PETRA): a newly defined lineage of mycobacterium tuberculosis in israel originating predominantly in Sub-Saharan Africa.
Freidlin PJ et al
Journal of Clinical Microbiology, 47(12), 4006-4020 (2009)
Effect of sward dry matter digestibility on methane production, ruminal fermentation, and microbial populations of zero-grazed beef cattle.
Hart KJ et al
Journal of Animal Science, 87(10), 3342-3350 (2009)
Gene expression signals involved in ischemic injury, extracellular matrix composition and fibrosis defined by global mRNA profiling of the human left ventricular myocardium.
Gabrielsen A et al
Journal of Molecular and Cellular Cardiology, 42(4), 870-883 (2007)
Comparison of coliforms and coliphages as tools for assessment of viral contamination in river water.
Skraber S et al
Applied and Environmental Microbiology, 70(6), 3644-3649 (2004)

商品

Viral RNA Extraction Buffer (VRE100) and related reagents for qRT-PCR and additional viral RNA analyses.

Quality DNA crucial for meaningful data; intact DNA key for successful chromosomal aberration analysis from FFPE tissues.

实验方案

The Imprint® Methylated DNA Quantification Kit provides a high-throughput, non-radioactive means of measuring global DNA methylation shifts.

Imprint®甲基化DNA定量试剂盒提供了一种高通量、非放射性的整体DNA甲基化变化测定方法。

GenomePlex® is a Whole Genome Amplification (WGA) method that allows the researcher to generate a representative amplification of genomic DNA

WTA2, a Whole Transcriptome Amplification (WTA) method, allows for representative amplification of nanogram quantities of total RNA in less than 4 hours without 3-bias

查看所有结果

相关内容

GenomePlex is a Whole Genome Amplification (WGA) method that allows the researcher to generate a representative, 500-fold amplification of genomic DNA

GenomePlex®全基因组扩增是从动物样品中提取DNA的方法。GenomePlex®产品已用于扩增鸡、猪、牛、鱼和虾来源的基因组DNA。

Questions

1–4 of 4 Questions  
  1. Do you have stability data for W4502 for evaluating if Shelf Life can be extended?

    1 answer
    1. This water product is not assigned an expiration or retest date. In the original sealed container the material is stable nearly indefinitely. Items that are not assigned an expiration date are warranteed for 1 year from the date of shipment.

      Helpful?

  2. Is this water sterile? Can this be used for cell culture?

    1 answer
    1. Product W4502 is not sterile, this product is prepared from Double Distilled and Deionized Water. This product is best suitable to use in molecular biology applications. See product W3500 for Sterile, Tissue Culture grade Water:
      https://www.sigmaaldrich.com/product/sigma/w3500

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  3. In what vessel does this product come? A bottle, a bag with a spigot, etc.?

    1 answer
    1. This product is packaged in square PET square bottles. To request a quote for larger volumes or specialized packaging, please submit a request at the link below:
      https://www.sigmaaldrich.com/services/support/bulk-quotation-request

      Helpful?

  4. This product does not list an expiration date on the label. Could someone provide an expiration for lot number BCCH7145?

    1 answer
    1. This product does not contain an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services

      Helpful?

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