W4502

Sigma-Aldrich

Nuclease-Free Water, for Molecular Biology

别名:
无核酸酶水, 未DEPC处理, 无RNase水, 无DNase水, 无蛋白酶水
线性分子式:
H2O
CAS号:
分子量:
18.02
Beilstein/REAXYS Number:
2050024
EC 号:
MDL编号:
PubChem化学物质编号:
NACRES:
NA.31

质量水平

200

蒸汽密度

<1 (vs air)

蒸汽压

3 mmHg

无菌性

0.1 μm filtered

形式

liquid

应用

PCR: suitable
electrophoresis: suitable

折射率

n20/D 1.34 (lit.)

pH值(酸碱度)

5-7

bp

100 °C (lit.)

mp

0 °C (lit.)

密度

1.000 g/mL at 3.98 °C (lit.)

异质活性

DNase, RNase, Protease, free

SMILES string

O

InChI

1S/H2O/h1H2

InChI key

XLYOFNOQVPJJNP-UHFFFAOYSA-N

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应用

水已用于-
  • cDNA合成和PCR扩增
  • 在qPCR检测前对瘤胃DNA样品进行稀释
  • PCR(聚合酶链反应)和琼脂糖凝胶电泳
  • RNA分离过程中RNA的洗脱

特点和优势

  • 无核酸酶水
  • 无DNase、RNase、蛋白酶
  • 未DEPC处理
  • 0.1 um过滤
  • 采用双蒸去离子水配制
  • 适于分子生物学应用

本品经过0.1um过滤。本品为分子生物学级试剂,适用于分子生物学应用。本产品针对核酸酶和蛋白酶的有无及微生物含量进行了分析,但未经焦碳酸二乙酯(DEPC)处理。

制备说明

该产品采用双蒸水和去离子水制备

其他说明

Easily compare specifications for Water products with the Water specification table.

storage_class_code

12 - Non Combustible Liquids

WGK Germany

nwg

闪点(F)

No data available

闪点(C)

No data available

个人防护装备

Eyeshields, Gloves

Comparison of coliforms and coliphages as tools for assessment of viral contamination in river water.
Skraber S et al
Applied and Environmental Microbiology, 70(6), 3644-3649 (2004)
Effect of sward dry matter digestibility on methane production, ruminal fermentation, and microbial populations of zero-grazed beef cattle.
Hart KJ et al
Journal of Animal Science, 87(10), 3342-3350 (2009)
Presence of viral genomes in mineral water: a sufficient condition to assume infectious risk?
Gassilloud B et al
Applied and Environmental Microbiology, 69(7), 3965-3969 (2003)
Polymorphic exact tandem repeat A (PETRA): a newly defined lineage of mycobacterium tuberculosis in israel originating predominantly in Sub-Saharan Africa.
Freidlin PJ et al
Journal of Clinical Microbiology, 47(12), 4006-4020 (2009)
Gene expression signals involved in ischemic injury, extracellular matrix composition and fibrosis defined by global mRNA profiling of the human left ventricular myocardium.
Gabrielsen A et al
Journal of Molecular and Cellular Cardiology, 42(4), 870-883 (2007)
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