跳转至内容
Merck

W4502

Sigma-Aldrich

suitable for electrophoresis, PCR

Water

别名:

无DNase水, 无RNase水, 无核酸酶水, 无蛋白酶水, 未DEPC处理

Slide 1 of 2
登录查看公司和协议定价

选择尺寸

1 L
$188.00
10 X 50 ML
$234.00
6 X 1 L
$842.00
10 L
$880.00

$188.00


即日发货详情


获取大包装/定制包装报价

关于此项目

线性分子式:
H2O
化学文摘社编号:
分子量:
18.02
Beilstein:
2050024
EC號碼:
MDL號碼:
分類程式碼代碼:
12191602
PubChem物質ID:
NACRES:
NA.31
Bp:
100 °C (lit.)

跳至


技术服务
需要帮助?我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助

产品名称

水, Nuclease-Free Water, for Molecular Biology

蒸汽密度

<1 (vs air)

品質等級

蒸汽壓力

3 mmHg

無菌

0.1 μm filtered

形狀

liquid

技術

PCR: suitable
electrophoresis: suitable

折射率

n20/D 1.34 (lit.)

pH值

5-7

bp

100 °C (lit.)

mp

0 °C (lit.)

密度

1.000 g/mL at 3.98 °C (lit.)

異物活動

DNase, RNase, Protease, free

SMILES 字串

O

InChI

1S/H2O/h1H2

InChI 密鑰

XLYOFNOQVPJJNP-UHFFFAOYSA-N

正在寻找类似产品? 访问 产品对比指南

比较类似商品

查看完整比较结果

显示差异

1 of 4

此商品
9801-OP9528995284
technique(s)

PCR: suitable, electrophoresis: suitable

technique(s)

-

technique(s)

-

technique(s)

FISH: suitable

Quality Level

200

Quality Level

100

Quality Level

100

Quality Level

100

form

liquid

form

liquid

form

liquid

form

liquid

mp

0 °C (lit.)

mp

0 °C (lit.)

mp

0 °C (lit.)

mp

0 °C (lit.)

refractive index

n20/D 1.34 (lit.)

refractive index

n20/D 1.34 (lit.)

refractive index

n20/D 1.34 (lit.)

refractive index

n20/D 1.34 (lit.)

density

1.000 g/mL at 3.98 °C (lit.)

density

1.000 g/mL at 3.98 °C (lit.)

density

1.000 g/mL at 3.98 °C (lit.)

density

1.000 g/mL at 3.98 °C (lit.)

應用

水已用于-
  • cDNA合成和PCR扩增[1][2]
  • 在qPCR检测前对瘤胃DNA样品进行稀释[3]
  • PCR(聚合酶链反应)和琼脂糖凝胶电泳[4]
  • RNA分离过程中RNA的洗脱[5]

特點和優勢

  • 无核酸酶水
  • 无DNase、RNase、蛋白酶
  • 未DEPC处理
  • 0.1 um过滤
  • 采用双蒸去离子水配制
  • 适于分子生物学应用

本品经过0.1um过滤。本品为分子生物学级试剂,适用于分子生物学应用。本产品针对核酸酶和蛋白酶的有无及微生物含量进行了分析,但未经焦碳酸二乙酯(DEPC)处理。

準備報告

该产品采用双蒸水和去离子水制备

其他說明

可根据水质参数表轻松比较产水参数。

相關產品

产品编号
说明
价格

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

nwg

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

没有发现合适的版本?

如果您需要特殊版本,可通过批号或批次号查找具体证书。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库

Polymorphic exact tandem repeat A (PETRA): a newly defined lineage of mycobacterium tuberculosis in israel originating predominantly in Sub-Saharan Africa.
Freidlin PJ et al
Journal of Clinical Microbiology, 47(12), 4006-4020 (2009)
Effect of sward dry matter digestibility on methane production, ruminal fermentation, and microbial populations of zero-grazed beef cattle.
Hart KJ et al
Journal of Animal Science, 87(10), 3342-3350 (2009)
Presence of viral genomes in mineral water: a sufficient condition to assume infectious risk?
Gassilloud B et al
Applied and Environmental Microbiology, 69(7), 3965-3969 (2003)
Gene expression signals involved in ischemic injury, extracellular matrix composition and fibrosis defined by global mRNA profiling of the human left ventricular myocardium.
Gabrielsen A et al
Journal of Molecular and Cellular Cardiology, 42(4), 870-883 (2007)
Comparison of coliforms and coliphages as tools for assessment of viral contamination in river water.
Skraber S et al
Applied and Environmental Microbiology, 70(6), 3644-3649 (2004)

商品

了解 mRNA 疫苗如何诱导免疫,以及如何为疫苗免疫原和其他生物制药制备合成 mRNA。寻找合成 mRNA 所需的试剂。

实验方案

Reverse transcription analyzes mRNA gene expression, facing challenges like transcript half-life differences and temporal patterns.

WTA2, a Whole Transcriptome Amplification (WTA) method, allows for representative amplification of nanogram quantities of total RNA in less than 4 hours without 3-bias

The 3’/5’ integrity assay identifies RNA degradation, crucial for large sample sets or less detectable degradation.

Standard qPCR protocol with up to four detection probes at specified concentrations, simplifying reaction setup with LuminoCt ReadyMix.

查看所有结果

相关内容

GenomePlex is a Whole Genome Amplification (WGA) method that allows the researcher to generate a representative, 500-fold amplification of genomic DNA

Questions

1–5 of 5 Questions  
  1. W4502 Millipore Water: what is the ohms of this water?

    1 answer
    1. The conductivity of this water is not determined. Currently, there is no Molecular Biology grade water product available that is tested for conductivity.

      Helpful?

  2. Do you have stability data for W4502 for evaluating if Shelf Life can be extended?

    1 answer
    1. This water product is not assigned an expiration or retest date. In the original sealed container the material is stable nearly indefinitely. Items that are not assigned an expiration date are warranteed for 1 year from the date of shipment.

      Helpful?

  3. Is this water sterile? Can this be used for cell culture?

    1 answer
    1. Product W4502 is not sterile, this product is prepared from Double Distilled and Deionized Water. This product is best suitable to use in molecular biology applications. See product W3500 for Sterile, Tissue Culture grade Water:
      https://www.sigmaaldrich.com/product/sigma/w3500

      Helpful?

  4. In what vessel does this product come? A bottle, a bag with a spigot, etc.?

    1 answer
    1. This product is packaged in square PET square bottles. To request a quote for larger volumes or specialized packaging, please submit a request at the link below:
      https://www.sigmaaldrich.com/services/support/bulk-quotation-request

      Helpful?

  5. This product does not list an expiration date on the label. Could someone provide an expiration for lot number BCCH7145?

    1 answer
    1. This product does not contain an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services

      Helpful?

Reviews

Active Filters

  1. USA
    • Review 1
    • Votes 3
    1 out of 5 stars.

    This water has microbial contamination!

    We used this water (Lot #0000387784) to dilute DNA for cell culture, but the cells kept getting contaminated. Eventually we traced it back to the water. Even a freshly opened bottle had microbial growth after mixing with culture medium. This ended up ruining thousands of dollars of experiments.

    Helpful?

    1. Response from Merck KGaA:

      Thank you for your review. We are sorry to hear that your experience did not match your product expectations. We encourage customers who experience problems with our products to contact us for additional technical support. Please visit https://www.sigmaaldrich.com/US/en/support/customer-support to submit a Product Technical Inquiry.

  2. 1 Ratings-only review

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系客户支持