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WGA2

Sigma-Aldrich

GenomePlex® 全基因组扩增 (WGA) Kit

Optimized kit with enzyme for amplifying a variety of DNA including FFPE tissue

NACRES:
NA.55

质量水平

200

technique(s)

whole genome amplification: suitable

运输

wet ice

储存温度

−20°C

一般描述

GenomePlex Complete Whole Genome Amplification Kit利用了我们的一项专利技术,该技术基于基因组DNA随机片段化的原理,所得的小片段转化为两侧含有通用引物位点的PCR扩增文库分子。WGA通过通用寡核苷酸引物进行的文库分子PCR扩增实现。

应用

GenomePlex®完整全基因组扩增(WGA)试剂盒用于DNA扩增.这一试剂盒还适用于下游应用,包括:
  • 芯片分析
  • SNP分析
  • STR分析
  • DNA存档

特点和优势

  • 起始材料:10 ng gDNA 或 受损DNA 或 1 μl 血液或颊拭子。
  • 预期产量:5 - 10 μg
  • 所需时间约3小时(热循环仪进行14次循环)
  • WGA DNA聚合酶提供更高的扩增准确性,从而使下游应用更加准确。
  • 完整表达整个基因组,等位基因偏差最小
  • 阴性对照反应中无扩增子
  • DNA来源:全血、口腔拭子、血卡、植物、土壤、&福尔马林固定石蜡包埋的组织(FFPE)

其他说明

此试剂盒中提供的通用引物序列被认为是专有的。

法律信息

本产品的使用受到如下一项或多项美国专利及其对应的境外专利声明保护:5,789,224, 5,618,711, 6,127,155以及与届满的美国专利号5,079,352对应的境外专利声明。购买本产品,即相当于依照境外的专利声明获得了一份受限制、不可转让的使用许可,即将此等数量的产品用于购买者内部的研究用途。我们未明确表示、暗示或以禁止反言的形式授予您任何其他专利声明下的权利、进行任何专利方法申请的权利、进行任何形式的商业服务的权利,包括但不限于出于收费或其他商业考虑而报告购买者的研究活动结果的权利。本产品仅适合于研究用途。如需用于Roche专利许可的诊断用途,需另外征得Roche许可。有关购买许可的更多信息,可联系Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA获取。
GenomePlex is a registered trademark of Takara Bio USA, Inc.

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书

  • W4502Water, Nuclease-Free Water, for Molecular Biology

储存分类代码

10 - Combustible liquids

WGK

WGK 3

  1. What is the major difference between WGA1 and WGA2 kits?

    Functionally, WGA1 and WGA2 kits are identical. The only difference between the two kits is that WGA2 is supplied with the WGA polymerase.

  2. If starting with fragmented DNA, what is the smallest size fragment which can be successfully amplified with Product WGA2, GenomePlex® Amplification Kit, and do I still need to do the fragmentation step?

    The kit works best for fragments 400 bp and larger. When starting with fragmented DNA, we recommend: (1) skipping the fragmentation heat step, although the buffer should be added, and (2) increasing the PCR cycles from 14 to 20.

  3. Can I use Product WGA2, GenomePlex® Amplification Kit, to amplify DNA from a single cell?

    Using this kit to amplify DNA from a single cell is not recommended.  We recommend using the GenomePlex Single Cell WGA Kit (WGA4) for such application.  WGA4 was developed for use with single cells and includes an optimized cell lysis protocol which has been incorporated into the fragmentation step.

  4. Can I amplify single stranded DNA with WGA2, GenomePlex® Amplification Kit?

    When starting with single stranded starting materials, we recommend (1) skipping the fragmentation heat step, although the buffer should be added and (2) increasing the PCR cycles from 14 to 20.  Note that if the ssDNA is actually cDNA from polyadenylated RNA, the kit will likely not give good representation of the input material, as the poly(T) ends constitute a large, non-random fraction.

  5. Is the WGA2 GenomePlex® Amplification Kit polymerase compatible with TA cloning?

    WGA polymerase is compatible with TA cloning with the following alteration to the PCR step: Be sure to include a 7 to 30 minute extension at 72°C after the last cycle to ensure that all PCR products are full length and 3´ adenylated.

  6. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  7. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Levi Yant et al.
The Plant cell, 22(7), 2156-2170 (2010-08-03)
The Arabidopsis thaliana transcription factor APETALA2 (AP2) has numerous functions, including roles in seed development, stem cell maintenance, and specification of floral organ identity. To understand the relationship between these different roles, we mapped direct targets of AP2 on a
Zhong Zhao et al.
Nature, 465(7301), 1089-1092 (2010-06-26)
The classic phytohormones cytokinin and auxin play essential roles in the maintenance of stem-cell systems embedded in shoot and root meristems, and exhibit complex functional interactions. Here we show that the activity of both hormones directly converges on the promoters
Comparative metagenomics of viral assemblages inhabiting four phyla of marine invertebrates
Gudenkauf BM and Hewson I
Frontiers in Marine Science, 3, 23-23 (2016)
Identification of key genes and pathways associated with different immune statuses of hepatitis B virus infection
Jin J, et al
Journal of Cellular and Molecular Medicine, 23, 7474-7489 (2019)
Jinglan Jin et al.
Journal of cellular and molecular medicine, 23(11), 7474-7489 (2019-10-01)
We aimed to identify key genes and pathways associated with different immune statuses of hepatitis B virus (HBV) infection. The gene expression and DNA methylation profiles were analysed in different immune statuses of HBV infection. Differentially expressed genes (DEGs) and

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动物组织DNA提取和WGA扩增实验方案

GenomePlex®全基因组扩增是从动物样品中提取DNA的方法。GenomePlex®产品已用于扩增鸡、猪、牛、鱼和虾来源的基因组DNA。

查看所有结果

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