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WGA4

Sigma-Aldrich

GenomePlex® 单细胞全基因组扩增试剂盒

Amplify genome of a single cell

EC 号:
NACRES:
NA.55

质量水平

200

应用

whole genome amplification: suitable

运输

wet ice

储存温度

−20°C

一般描述

GenomePlex Single Cell Whole Genome Amplification Kit利用了我们的一项专利技术,该技术基于基因组DNA随机片段化的原理,所得的小片段转化为两侧含有通用引物位点的PCR扩增文库分子。WGA通过通用寡核苷酸引物进行的文库分子PCR扩增实现。

应用

GenomePlex®单细胞全基因组扩增试剂盒已用于:
  • 扩增分离后的DNA
  • 全基因组扩增(WGA)
  • 扩增显微切片DNA

特点和优势

  • 起始材料:1个细胞
  • 预期得率:5 - 10 μg
  • 所需时间:约4小时
  • 可在四小时内获得高丰度的DNA
  • 可使整个基因组得到完整扩增,且可最大限度降低等位基因偏倚
  • 包含进行细胞裂解及之后的全基因组扩增所需的所有必要试剂
  • 可用于扩增任何来源的单细胞基因组,包括淋巴细胞、癌细胞、上皮细胞、成纤维细胞、羊膜细胞、聚碳酸酯固定细胞以及植物细胞。

其他说明

该试剂盒随附的通用引物的序列组成具有专利权。

法律信息

本产品的使用受到如下一项或多项美国专利及其对应的境外专利声明保护:5,789,224, 5,618,711, 6,127,155以及与届满的美国专利号5,079,352对应的境外专利声明。购买本产品,即相当于依照境外的专利声明获得了一份受限制、不可转让的使用许可,即将此等数量的产品用于购买者内部的研究用途。我们未明确表示、暗示或以禁止反言的形式授予您任何其他专利声明下的权利、进行任何专利方法申请的权利、进行任何形式的商业服务的权利,包括但不限于出于收费或其他商业考虑而报告购买者的研究活动结果的权利。本产品仅适合于研究用途。如需用于Roche专利许可的诊断用途,需另外征得Roche许可。有关购买许可的更多信息,可联系Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA获取。
GenomePlex is a registered trademark of Takara Bio USA, Inc.

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书

  • L104310x Single Cell Lysis & Fragmentation Buffer

  • P4850Proteinase K from Tritirachium album, buffered aqueous glycerol solution, for molecular biology, ≥800 units/mL

  • W4502Water, Nuclease-Free Water, for Molecular Biology

象形图

Health hazard

警示用语:

Danger

危险声明

预防措施声明

危险分类

Resp. Sens. 1

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. Can the amplified Omniplex products resulting from the use of Product WGA4, GenomePlex® Single Cell Whole Genome Amplification Kit, be used for TA cloning?

    Yes.  Please ensure that a final extension of 7-30 minutes at 72°C is included after amplification if performing subsequent TA cloning reactions.

  4. Can Product WGA4, GenomePlex® Single Cell Whole Genome Amplification Kit, be used with purified DNA?

    Yes. The kit is designed to amplify the small amount of DNA that is present in a single cell (3-5 pg). It will also work with small amounts of purified DNA; we recommend less than 100 pg.

  5. What kinds of cells can be used as starting materials with Product WGA4, GenomePlex® Single Cell Whole Genome Amplification Kit?

    The cell lysis method involves treatment with proteinase K and heat. Most cells will be lysed under these conditions.

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Nicholas Navin et al.
Nature, 472(7341), 90-94 (2011-03-15)
Genomic analysis provides insights into the role of copy number variation in disease, but most methods are not designed to resolve mixed populations of cells. In tumours, where genetic heterogeneity is common, very important information may be lost that would
Timour Baslan et al.
eLife, 9 (2020-05-14)
Copy number alterations (CNAs) play an important role in molding the genomes of breast cancers and have been shown to be clinically useful for prognostic and therapeutic purposes. However, our knowledge of intra-tumoral genetic heterogeneity of this important class of
Jochen B Geigl et al.
Nature protocols, 2(12), 3173-3184 (2007-12-15)
A comprehensive genomic analysis of single cells is instrumental for numerous applications in tumor genetics, clinical diagnostics and forensic analyses. Here, we provide a protocol for single-cell isolation and whole genome amplification, which includes the following stages: preparation of single-cell
Nathan R Treff et al.
Fertility and sterility, 94(6), 2017-2021 (2010-03-02)
To develop and validate a whole genome amplification and single nucleotide polymorphism (SNP) microarray protocol for accurate single cell 24 chromosome aneuploidy screening. Prospective, randomized, and blinded study. Academic reproductive medicine center. Multiple euploid and aneuploid cell lines were obtained
Heike Fiegler et al.
Nucleic acids research, 35(3), e15-e15 (2006-12-21)
Heterogeneity in the genome copy number of tissues is of particular importance in solid tumor biology. Furthermore, many clinical applications such as pre-implantation and non-invasive prenatal diagnosis would benefit from the ability to characterize individual single cells. As the amount

实验方案

Whole Genome Amplification from Serum or Plasma Protocol

Whole genome amplification (WGA) of plasma and serum DNA presents a unique challenge due to the small amount of nucleic acid in such samples.

Buccal DNA Extraction & WGA Amplification Protocol

This protocol provides a simple and convenient method to isolate, amplify, and purify genomic DNA from buccal swabs. Buccal swabs are a convenient method of acquiring a DNA sample.

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