whole genome amplification: suitable
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
The dNTP Mix (D6193) is supplied as 10 mM each.
Using the kit as is, Ribosomal RNA does appear to amplify. However, we have not quantitated how well it is amplified.
The starting material for WTA is RNA, whereas for WGA it is genomic DNA. After the RNA is reverse-transcribed into the DNA complement by WTA, the OmniPlex library is formed. This library is in turn amplified by PCR.
Yes, the WTA kit effectively amplifies degraded RNA, including formalin-fixed and paraffin-embedded samples.
Upon creation of a cDNA library, quantitative qPCR or microarray analysis may be performed
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Amplification products generated by the TransPlex® WTA and Complete WTA2 kits are suitable for microarray target for expression analyses, and can be readily integrated into existing NimbleGen workflows.
Transplex Whole Transcriptome Amplification FAQs on topics including whole transcriptome steps, RNA source, including archival fixed tissue, library purification, quantitation of the product and downstream applications
TransPlex WTA amplification product is suitable as microarray target for expression analysis on the Affymetrix platform, and can be incorporated into existing Affymetrix workflows
Amplification products generated by the TransPlex® WTA and Complete WTA2 kits are suitable for microarray target for expression analyses, and can be incorporated into existing Illumina workflows.