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WTA1

Sigma-Aldrich

TransPlex® Whole Transcriptome Amplification Kit

DNA polymerase separate.

别名:
TransPlex®全转录组扩增试剂盒
NACRES:
NA.55

质量水平

200

应用

whole genome amplification: suitable

运输

wet ice

储存温度

−20°C

一般描述

TransPlex是一种全转录组扩增(WTA)方法,可在4小时内对ng级的总RNA进行无3′ 偏差的代表性扩增。以组织、培养的细胞、福尔马林固定样品或血清为原料生成µg级扩增产物,适用于qPCR和微阵列分析等下游应用。

应用

TransPlex® 全转录组扩增试剂盒已用于合成双链 cDNA。
适于各种下游应用,包括:
  • qPCR
  • 微阵列分析
  • 克隆

特点和优势

  • 在4小时内对总RNA进行扩增,手动操作时间低于30分钟
  • 仅需5 ng起始材料,即可从总RNA扩增为极具代表性的文库
  • 以组织、培养的细胞、血清或来自福尔马林固定的石蜡包埋样品的降解RNA为原料,从完整的RNA可生成微克级的扩增产物

原理

WTA过程包括2个步骤。首先,使用由拟随机3′端和通用5′端组成的非自身互补引物对样品RNA进行逆转录。随着聚合作用的发生,被取代的单链将作为引物退火和延伸的新模板。所得OmniPlex cDNA文库由以通用末端序列为侧翼序列、含100-1000个碱基的随机重叠片段组成。随后,使用通用引物对文库进行PCR扩增,生成WTA产物。

法律信息

TransPlex is a registered trademark of Rubicon Genomics, Inc.

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书

  • W4502Water, Nuclease-Free Water, for Molecular Biology

  • D7295Deoxynucleotide Mix, 10 mM, Molecular Biology Reagent

象形图

Exclamation mark

警示用语:

Warning

危险声明

危险分类

Eye Irrit. 2

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable

分析证书

原产地证书 (CofO)

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is the concentration of the dNTPs supplied with the TransPlex® Whole Transcriptome Amplification Kit, Product WTA1?

    The dNTP Mix (D6193) is supplied as 10 mM each.

  4. Is the TransPlex® Whole Transcriptome Amplification Kit, Product WTA1, also applicable for ribosomal RNA and/or small RNA amplification?

    Using the kit as is, Ribosomal RNA does appear to amplify. However, we have not quantitated how well it is amplified.

  5. What is the difference between WTA and WGA?   I use the TransPlex® Whole Transcriptome Amplification Kit, Product WTA1.

    The starting material for WTA is RNA, whereas for WGA it is genomic DNA.  After the RNA is reverse-transcribed into the DNA complement by WTA, the OmniPlex library is formed. This library is in turn amplified by PCR.

  6. Can TransPlex® Whole Transcriptome Amplification Kit, Product WTA1, be used to amplify archival fixed tissue or degraded samples?

    Yes, the WTA kit effectively amplifies degraded RNA, including formalin-fixed and paraffin-embedded samples.

  7. Can TransPlex® Whole Transcriptome Amplification Kit, Product WTA1, be used for downstream applications?

    Upon creation of a cDNA library, quantitative qPCR or microarray analysis may be performed

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Ki Wook Kim et al.
Open forum infectious diseases, 6(2), ofz025-ofz025 (2019-03-01)
The importance of gut bacteria in human physiology, immune regulation, and disease pathogenesis is well established. In contrast, the composition and dynamics of the gut virome are largely unknown; particularly lacking are studies in pregnancy. We used comprehensive virome capture
Sara Planamente et al.
Molecular microbiology, 86(5), 1085-1099 (2012-10-10)
GABA acts as an intercellular signal in eukaryotes and as an interspecies signal in host-microbe interactions. Structural characteristics of selective eukaryotic GABA receptors and bacterial GABA sensors are unknown. Here, we identified the selective GABA-binding protein, called Atu4243, in the
Anne H Rowley et al.
The Journal of infectious diseases, 203(7), 1021-1030 (2011-03-16)
Intracytoplasmic inclusion bodies (ICI) have been identified in ciliated bronchial epithelium of Kawasaki disease (KD) patients using a synthetic antibody derived from acute KD arterial IgA plasma cells; ICI may derive from the KD etiologic agent. Acute KD bronchial epithelium
Eszter Posfai et al.
Genes & development, 26(9), 920-932 (2012-04-14)
In mammals, totipotent embryos are formed by fusion of highly differentiated gametes. Acquisition of totipotency concurs with chromatin remodeling of parental genomes, changes in the maternal transcriptome and proteome, and zygotic genome activation (ZGA). The inefficiency of reprogramming somatic nuclei
Munehiro Okamoto et al.
Scientific reports, 5, 8850-8850 (2015-03-07)
We discovered a lethal hemorrhagic syndrome arising from severe thrombocytopenia in Japanese macaques kept at the Primate Research Institute, Kyoto University. Extensive investigation identified that simian retrovirus type 4 (SRV-4) was the causative agent of the disease. SRV-4 had previously

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